{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,24]],"date-time":"2025-09-24T10:16:58Z","timestamp":1758709018753,"version":"3.37.3"},"reference-count":46,"publisher":"Scientific Foundation Spiroski (publications)","issue":"1","license":[{"start":{"date-parts":[[2015,12,25]],"date-time":"2015-12-25T00:00:00Z","timestamp":1451001600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Open Access Maced J Med Sci"],"abstract":"<jats:p>BACKGROUND: The proportion of T and B lymphocytes in pleural fluids and blood may point to the presence of local immunological phenomena in pleural disorders.AIM: Aim of study was to evaluate the lymphocyte phenotype and the ratio between helper (CD4+) and cytotoxic\/suppressor (CD8+) lymphocytes in malignant and non-malignant effusions.MATERIAL AND METHODS: We studied 48 patients with pleural effusions. First group had 18 patients with tuberculosis pleural effusions; second group had 20 patients with malignant pleural fluids, third group had 10 patients with transudates and 30 healthy controls. We investigated the distribution of T and B lymphocytes, T cells with helper\/inducer CD4 or suppresser\/cytotoxic CD8 phenotypes and the CD16 subset.RESULTS: Results showed decreases levels of CD3, CD4, and CD16 T cells in blood of patients versus healthy controls. There were increases in the percentage of the CD3 and CD4 T cells in the pleural fluid compared with values in the blood with statistical significance in tuberculous pleurisy. The values of CD8 were similar in the pleural fluid and in blood. Levels of CD16 were non-significantly higher in pleural fluid in all groups.CONCLUSION: This study confirms the hypothesis that pleural cavity is compartment with immunological reactivity and results could be used in differential diagnosis together with other examinations.<\/jats:p>","DOI":"10.3889\/oamjms.2016.009","type":"journal-article","created":{"date-parts":[[2015,12,26]],"date-time":"2015-12-26T09:27:30Z","timestamp":1451122050000},"page":"50-53","source":"Crossref","is-referenced-by-count":2,"title":["Analysis of Lymphocyte Immunological Reactivity in Patients with Pleural Effusions of Different Etiology"],"prefix":"10.3889","volume":"4","author":[{"given":"Zlatica","family":"Goseva","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Biserka","family":"Jovkovska Kaeva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angelko","family":"Gjorcev","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena","family":"Jovanovska Janeva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zoran","family":"Arsovski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sava","family":"Pejkovska","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aleksandra","family":"Tatabitovska","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"52292","published-online":{"date-parts":[[2015,12,25]]},"reference":[{"key":"17196","doi-asserted-by":"crossref","unstructured":"Dalbeth N, Lee YCG. Lymphocytes in pleural disease. Current Opinion in Pulmonary Medicine. 2005;11(4):334-339.","DOI":"10.1097\/01.mcp.0000166490.92659.17"},{"key":"17197","doi-asserted-by":"crossref","unstructured":"http:\/\/dx.doi.org\/10.1097\/01.mcp.0000166490.92659.17","DOI":"10.1097\/01.mcp.0000166490.92659.17"},{"key":"17198","unstructured":"PMid:15928502"},{"key":"17199","doi-asserted-by":"crossref","unstructured":"Aguiar LMZ de, Antonangelo L, Vargas FS, Zerbini MCN, Sales MM, Uip DE, Saldiva PHN. Malignant and tuberculous pleural effusions: immunophenotypic cellular characterization. Clinics. 2008;63(5):637-44.","DOI":"10.1590\/S1807-59322008000500012"},{"key":"17200","doi-asserted-by":"crossref","unstructured":"http:\/\/dx.doi.org\/10.1590\/S1807-59322008000500012","DOI":"10.1590\/S1807-59322008000500012"},{"key":"17201","unstructured":"PMCid:PMC2664722"},{"key":"17202","unstructured":"Kochman S, Bernard J, Lavaud F, Cazabat A, Dubois Montreynaud JM. T lymphocyte subsets in pleural fluids: discrimination according to traditional and monoclonal antibody-defined markers. Eur J Resp Dis. 1984;65:586-591."},{"key":"17203","unstructured":"PMid:6240409"},{"key":"17204","doi-asserted-by":"crossref","unstructured":"Lucivero G, Pierucci G, Bonamo L. Lymphocyte subsets in peripheral blood and pleural fluid. Eur Resp J. 1988;1:337-340.","DOI":"10.1183\/09031936.93.01040337"},{"key":"17205","unstructured":"PMid:3260873"},{"key":"17206","doi-asserted-by":"crossref","unstructured":"Vald\u00c3\u00a9s L, Pose A, San Jos\u00c3\u00a9 E, Mart\u00c3\u00adnez V\u00c3\u00a1zquez JM. Tuberculous pleural effusions. Eur J Intern Med. 2003;14(2):77-88.","DOI":"10.1016\/S0953-6205(03)00018-9"},{"key":"17207","doi-asserted-by":"crossref","unstructured":"http:\/\/dx.doi.org\/10.1016\/S0953-6205(03)00018-9","DOI":"10.1016\/S0953-6205(03)00018-9"},{"key":"17208","doi-asserted-by":"crossref","unstructured":"Masakazu O,Yoshinori H, Toru H, Naozumi H, Kazuyoshi I, Kaoru Sh, Tsutomu K. T-Helper Type 1\/T-Helper Type 2 Balance in Malignant Pleural Effusions Compared to Tuberculous Pleural Effusions. CHEST. 2005;128:4030\u00e2\u20ac\u201c4035.","DOI":"10.1378\/chest.128.6.4030"},{"key":"17209","doi-asserted-by":"crossref","unstructured":"http:\/\/dx.doi.org\/10.1378\/chest.128.6.4030","DOI":"10.1378\/chest.128.6.4030"},{"key":"17210","unstructured":"PMid:16354877"},{"key":"17211","doi-asserted-by":"crossref","unstructured":"Atanackovic Dj, Block A, De Weerth A, Faltz C, Hossfeld DK, Hegewisch-Becker S. Characterization of Effusion-Infiltrating T Cells: Benign versus Malignant Effusions. Clinical Cancer Research. 2004;10:2600\u00e2\u20ac\u201c2608.","DOI":"10.1158\/1078-0432.CCR-03-0239"},{"key":"17212","doi-asserted-by":"crossref","unstructured":"http:\/\/dx.doi.org\/10.1158\/1078-0432.CCR-03-0239","DOI":"10.1158\/1078-0432.CCR-03-0239"},{"key":"17213","unstructured":"Shiratsuchi H, Tsuyguchi J. Analysis of T cell subsets by monoclonal antibodies in patients with tuberculosis after in vitro stimulation with purified protein derivate of tuberculin. Clin Exp Immunol. 1984; 57:271-278."},{"key":"17214","unstructured":"PMid:6331920 PMCid:PMC1536122"},{"key":"17215","unstructured":"Ghosha AG, Mukherjee K, Saha AK, Chowdhury TK. Compartmentalisation of lymphocytes in tuberculous pleural effusion: a preliminary observation. Ind J Tub. 1996;43:23."},{"key":"17216","unstructured":"Klimiuk J, Domaga\u00c5\u201aa-Kulawik J, Krenke R, Chazan R. Lymphocyte and lymphocyte subsets in pleural fluid - comparison of malignant and non-malignant disorders. Pol Arch Med Wewn. 2004; 111(3):291-6."},{"key":"17217","unstructured":"PMid:15230209"},{"key":"17218","doi-asserted-by":"crossref","unstructured":"Barnes PF, Mistry SD, Cooper CL, Pirmez C, Rea TH, Modlin RL. Compartmentalization of a CD4+ T lymphocyte subpopulation in tuberculous pleuritis. J Immunol. 1989;142(4):1114-9.","DOI":"10.4049\/jimmunol.142.4.1114"},{"key":"17219","unstructured":"PMid:2464638"},{"key":"17220","doi-asserted-by":"crossref","unstructured":"Trajman A, Pai M, Dheda K, van Zyl Smit R, Zwerling AA, Joshi R, Kalantri S, Daley P, Menzies D. Novel tests for diagnosing tuberculous pleural effusion: what works and what does not? Eur Respir J. 2008;31(5):1098-106.","DOI":"10.1183\/09031936.00147507"},{"key":"17221","doi-asserted-by":"crossref","unstructured":"http:\/\/dx.doi.org\/10.1183\/09031936.00147507","DOI":"10.1183\/09031936.00147507"},{"key":"17222","unstructured":"PMid:18448504"},{"key":"17223","doi-asserted-by":"crossref","unstructured":"Tong ZH, Shi HZ. Subpopulations of helper T lymphocytes in tuberculous pleurisy. Tuberculosis. 2013;93(3):279-84.","DOI":"10.1016\/j.tube.2013.02.014"},{"key":"17224","doi-asserted-by":"crossref","unstructured":"http:\/\/dx.doi.org\/10.1016\/j.tube.2013.02.014","DOI":"10.1016\/j.tube.2013.02.014"},{"key":"17225","unstructured":"PMid:23477939"},{"key":"17226","doi-asserted-by":"crossref","unstructured":"San Jos\u00c3\u00a9 ME, Vald\u00c3\u00a9s L, Saavedra MJ, De Vega JM, Alvarez D, Vi-uela J, Penela P, Valle JM, Seoane R. Lymphocyte populations in tuberculous pleural effusions. Ann Clin Biochem. 1999;36(Pt 4):492-500.","DOI":"10.1177\/000456329903600413"},{"key":"17227","doi-asserted-by":"crossref","unstructured":"http:\/\/dx.doi.org\/10.1177\/000456329903600413","DOI":"10.1177\/000456329903600413"},{"key":"17228","unstructured":"PMid:10456212"},{"key":"17229","doi-asserted-by":"crossref","unstructured":"Qin XJ, Shi HZ, Liang QL, Huang LY, Yang HB. CD4+CD25+ regulatory T lymphocytes in tuberculous pleural effusion. Chin Med J (Engl). 2008;121(7):581-6.","DOI":"10.1097\/00029330-200804010-00002"},{"key":"17230","doi-asserted-by":"crossref","unstructured":"Robinson E, Segal R, Vesely Z. Lymphocyte subpopulation in peripheral blood and malignant effusions of cancer patients. Eur J Cancer Clin Oncol. 1986;22(2):191-193.","DOI":"10.1016\/0277-5379(86)90029-5"},{"key":"17231","doi-asserted-by":"crossref","unstructured":"http:\/\/dx.doi.org\/10.1016\/0277-5379(86)90029-5","DOI":"10.1016\/0277-5379(86)90029-5"},{"key":"17232","doi-asserted-by":"crossref","unstructured":"Chen YQ, Shi HZ, Qin XJ, Mo WN, Liang XD, Huang ZX, Yang HB, Wu C. CD4+CD25+ regulatory T lymphocytes in malignant pleural effusion. Am J Respir Crit Care Med. 2005;172(11):1434-9.","DOI":"10.1164\/rccm.200504-588OC"},{"key":"17233","doi-asserted-by":"crossref","unstructured":"http:\/\/dx.doi.org\/10.1164\/rccm.200504-588OC","DOI":"10.1164\/rccm.200504-588OC"},{"key":"17234","unstructured":"PMid:16151041"},{"key":"17235","doi-asserted-by":"crossref","unstructured":"Michael RS, David WE. T lymphocytes and pleural tuberculosis. Chest. 1986;89:4.","DOI":"10.1378\/chest.89.4.473"},{"key":"17236","doi-asserted-by":"crossref","unstructured":"Scherpereel A, Grigoriu BD, Noppen M, Gey T, Chahine B, Baldacci S, Trauet J, Copin MC, Dessaint JP, Porte H, Labalette M. Defect in recruiting effector memory CD8+ T-cells in malignant pleural effusions compared to normal pleural fluid. BMC Cancer. 2013;13:324.","DOI":"10.1186\/1471-2407-13-324"},{"key":"17237","doi-asserted-by":"crossref","unstructured":"http:\/\/dx.doi.org\/10.1186\/1471-2407-13-324","DOI":"10.1186\/1471-2407-13-324"},{"key":"17238","unstructured":"PMid:23816056 PMCid:PMC3718618"},{"key":"17239","doi-asserted-by":"crossref","unstructured":"Onishi H, Morisaki T, Kuga H, Katano M, Doi F, Uchiyama A, Sugitani A, Wada J, Chijiiwa K, Tanaka M. A large quantity of CD3-\/CD19-\/CD16- lymphocytes in malignant pleural effusion from a patient with recurrent cholangio cell carcinoma. Immunol Invest. 2002;31(2):121-35.","DOI":"10.1081\/IMM-120004803"},{"key":"17240","doi-asserted-by":"crossref","unstructured":"http:\/\/dx.doi.org\/10.1081\/IMM-120004803","DOI":"10.1081\/IMM-120004803"},{"key":"17241","unstructured":"PMid:12148948"}],"container-title":["Open Access Macedonian Journal of Medical Sciences"],"original-title":[],"link":[{"URL":"https:\/\/oamjms.eu\/index.php\/mjms\/article\/download\/oamjms.2016.009\/646","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/oamjms.eu\/index.php\/mjms\/article\/download\/oamjms.2016.009\/864","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,16]],"date-time":"2023-08-16T08:46:15Z","timestamp":1692175575000},"score":1,"resource":{"primary":{"URL":"https:\/\/oamjms.eu\/index.php\/mjms\/article\/view\/oamjms.2016.009"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,12,25]]},"references-count":46,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2016,3,15]]}},"URL":"https:\/\/doi.org\/10.3889\/oamjms.2016.009","relation":{},"ISSN":["1857-9655"],"issn-type":[{"type":"electronic","value":"1857-9655"}],"subject":[],"published":{"date-parts":[[2015,12,25]]}}}