{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T23:30:25Z","timestamp":1788219025538,"version":"build-2803163510"},"reference-count":0,"publisher":"Scientific Foundation Spiroski (publications)","issue":"8","license":[{"start":{"date-parts":[[2018,8,5]],"date-time":"2018-08-05T00:00:00Z","timestamp":1533427200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Open Access Maced J Med Sci"],"abstract":"<jats:p>AIMS: To evaluate three in vitro phenotypic methods; tissue culture plate, tube method, and Congo red agar for detection of biofilm formation in staphylococci and assess the relation of biofilm formation with methicillin resistance and anti-microbial resistance.\r\nMETHODS: The study included 150 staphylococcal isolates. Biofilm detection in staphylococci was performed using tissue culture plate, tube method, and Congo red agar.\r\nRESULTS: Tissue culture plate, tube method, and Congo red agar detected 74%, 42.7%, and 1.3% biofilm producing staphylococci respectively. S. aureus isolates were more common biofilm producers (53.2%) than CONS (46.8%). Biofilm production in CONS species was highest in S. hemolyticus (57.7%). Tube method was 51.4% sensitive, 82.1% specific. As for Congo red agar, sensitivity was very low (0.9%), but specificity was 97.4%. Biofilm producers were mostly; isolated from blood specimens (82.6%) and detected in methicillin-resistant strains 96\/111 (86.5%). They were resistant to most antibiotics except vancomycin and linezolid.\r\nCONCLUSIONS: Tissue culture plate is a more quantitative and reliable method for detection of biofilm producing staphylococci compared to tube method and Congo red agar. Hence, it can still be used as a screening method for biofilm detection. Vancomycin and Linezolid are the most sensitive antibiotics among biofilm producing staphylococci.<\/jats:p>","DOI":"10.3889\/oamjms.2018.246","type":"journal-article","created":{"date-parts":[[2018,8,11]],"date-time":"2018-08-11T04:30:11Z","timestamp":1533961811000},"page":"1335-1341","source":"Crossref","is-referenced-by-count":40,"title":["Detection of Biofilm Producing Staphylococci among Different Clinical Isolates and Its Relation to Methicillin Susceptibility"],"prefix":"10.3889","volume":"6","author":[{"given":"Rania M.","family":"Abdel Halim","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nevine N.","family":"Kassem","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Basma S.","family":"Mahmoud","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"52292","published-online":{"date-parts":[[2018,8,5]]},"container-title":["Open Access Macedonian Journal of Medical Sciences"],"original-title":[],"link":[{"URL":"https:\/\/spiroski.migration.publicknowledgeproject.org\/index.php\/mjms\/article\/download\/oamjms.2018.246\/2322","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/oamjms.eu\/index.php\/mjms\/article\/download\/oamjms.2018.246\/2287","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,19]],"date-time":"2023-04-19T21:25:24Z","timestamp":1681939524000},"score":1,"resource":{"primary":{"URL":"https:\/\/spiroski.migration.publicknowledgeproject.org\/index.php\/mjms\/article\/view\/oamjms.2018.246"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,8,5]]},"references-count":0,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2018,8,20]]}},"URL":"https:\/\/doi.org\/10.3889\/oamjms.2018.246","relation":{},"ISSN":["1857-9655"],"issn-type":[{"value":"1857-9655","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,8,5]]}}}