{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T02:02:29Z","timestamp":1775181749286,"version":"3.50.1"},"reference-count":58,"publisher":"Spandidos Publications","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomed Rep"],"DOI":"10.3892\/br.2024.1757","type":"journal-article","created":{"date-parts":[[2024,3,4]],"date-time":"2024-03-04T06:58:55Z","timestamp":1709535535000},"source":"Crossref","is-referenced-by-count":1,"title":["Cell\u2011free fetal DNA at\u00a011\u201113\u00a0weeks of gestation is not altered in complicated pregnancies"],"prefix":"10.3892","volume":"20","author":[{"given":"Zoi","family":"Koukou","sequence":"first","affiliation":[{"name":"School of Health Sciences, International Hellenic University (IHU), 57400 Thessaloniki, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eleftherios","family":"Panteris","sequence":"additional","affiliation":[{"name":"Laboratory of Forensic Medicine and Toxicology, School of Medicine, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emmanouel","family":"Manolakos","sequence":"additional","affiliation":[{"name":"Access to Genome P.C., Clinical Laboratory Genetics, 11528 Thessaloniki, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aristeidis","family":"Papadopoulos","sequence":"additional","affiliation":[{"name":"School of Medicine, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ioannis","family":"Papoulidis","sequence":"additional","affiliation":[{"name":"Access to Genome P.C., Clinical Laboratory Genetics, 11528 Thessaloniki, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Konstantinos","family":"Relakis","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, University Hospital of Heraklion, 71500 Heraklion, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stavros","family":"Sifakis","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, University Hospital of Heraklion, 71500 Heraklion, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2249","published-online":{"date-parts":[[2024,3,4]]},"reference":[{"key":"key20240328124155_b1-BR-20-4-01757","doi-asserted-by":"publisher","first-page":"245","DOI":"10.1016\/j.ogc.2017.02.004","article-title":"Prenatal diagnosis: Screening and diagnostic tools","volume":"44","author":"Carlson","year":"2017","journal-title":"Obstet Gynecol Clin North Am"},{"key":"key20240328124155_b2-BR-20-4-01757","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1007\/978-1-4939-8889-1_1","article-title":"Traditional prenatal diagnosis: Past to present","volume":"1885","author":"mLevy","year":"2019","journal-title":"Methods Mol Biol"},{"issue":"15","key":"key20240328124155_b3-BR-20-4-01757","doi-asserted-by":"publisher","DOI":"10.3390\/genes12010015","article-title":"Non-invasive prenatal testing: Current perspectives and future challenges","volume":"12","author":"Carbone","year":"2021","journal-title":"Genes (Basel)"},{"key":"key20240328124155_b4-BR-20-4-01757","doi-asserted-by":"publisher","first-page":"319","DOI":"10.1515\/jpm-2011-0282","article-title":"Noninvasive prenatal diagnosis of Down syndrome: Current knowledge and novel insights","volume":"40","author":"Sifakis","year":"2012","journal-title":"J Perinat Med"},{"key":"key20240328124155_b5-BR-20-4-01757","doi-asserted-by":"publisher","first-page":"485","DOI":"10.1016\/S0140-6736(97)02174-0","article-title":"Presence of fetal DNA in maternal plasma and serum","volume":"350","author":"Lo","year":"1997","journal-title":"Lancet"},{"key":"key20240328124155_b6-BR-20-4-01757","doi-asserted-by":"publisher","first-page":"218","DOI":"10.1086\/302205","article-title":"Rapid clearance of fetal DNA from maternal plasma","volume":"64","author":"Lo","year":"1999","journal-title":"Am J Hum Genet"},{"key":"key20240328124155_b7-BR-20-4-01757","doi-asserted-by":"publisher","first-page":"16266","DOI":"10.1073\/pnas.0808319105","article-title":"Noninvasive diagnosis of fetal aneuploidy by shotgun sequencing DNA from maternal blood","volume":"105","author":"Fan","year":"2008","journal-title":"Proc Natl Acad Sci USA"},{"key":"key20240328124155_b8-BR-20-4-01757","doi-asserted-by":"publisher","first-page":"472.e1","DOI":"10.1016\/j.ajog.2009.05.025","article-title":"First-trimester maternal plasma cell-free fetal DNA and preeclampsia","volume":"201","author":"Sifakis","year":"2009","journal-title":"Am J Obstet Gynecol"},{"key":"key20240328124155_b9-BR-20-4-01757","doi-asserted-by":"publisher","first-page":"2367","DOI":"10.3892\/mmr.2014.3118","article-title":"Cell-free fetal DNA and pregnancy-related complications (review)","volume":"11","author":"Sifakis","year":"2015","journal-title":"Mol Med Rep"},{"issue":"2816","key":"key20240328124155_b10-BR-20-4-01757","doi-asserted-by":"publisher","DOI":"10.3390\/diagnostics12112816","article-title":"Non-invasive prenatal testing in Germany","volume":"12","author":"Liehr","year":"2022","journal-title":"Diagnostics (Basel)"},{"key":"key20240328124155_b11-BR-20-4-01757","doi-asserted-by":"publisher","first-page":"840","DOI":"10.1002\/pd.3917","article-title":"High levels of fetal DNA are associated with increased risk of spontaneous preterm delivery","volume":"32","author":"Jakobsen","year":"2012","journal-title":"Prenat Diagn"},{"key":"key20240328124155_b12-BR-20-4-01757","doi-asserted-by":"publisher","first-page":"R137","DOI":"10.1530\/REP-17-0619","article-title":"Cell-free fetal DNA and spontaneous preterm birth","volume":"155","author":"van Boeckel","year":"2018","journal-title":"Reproduction"},{"key":"key20240328124155_b13-BR-20-4-01757","doi-asserted-by":"publisher","first-page":"1667","DOI":"10.1373\/49.10.1667","article-title":"Relationship between severity of hyperemesis gravidarum and fetal DNA concentration in maternal plasma","volume":"49","author":"Sugito","year":"2003","journal-title":"Clin Chem"},{"key":"key20240328124155_b14-BR-20-4-01757","doi-asserted-by":"publisher","first-page":"98.e91","DOI":"10.1016\/j.ajog.2008.07.063","article-title":"Quantification of cell free fetal DNA in maternal plasma in normal pregnancies and in pregnancies with placental dysfunction","volume":"200","author":"Alberry","year":"2009","journal-title":"Am J Obstet Gynecol"},{"key":"key20240328124155_b15-BR-20-4-01757","doi-asserted-by":"publisher","first-page":"1540","DOI":"10.1373\/49.9.1540","article-title":"Placenta increta: Postpartum monitoring of plasma cell-free fetal DNA","volume":"49","author":"Jimbo","year":"2003","journal-title":"Clin Chem"},{"key":"key20240328124155_b16-BR-20-4-01757","doi-asserted-by":"crossref","first-page":"787","DOI":"10.12891\/ceog1982.2015","article-title":"Cell free fetal DNA in the plasma of pregnant women with preeclampsia","volume":"42","author":"Seval","year":"2025","journal-title":"Clin Exp Obstet Gynecol"},{"key":"key20240328124155_b17-BR-20-4-01757","doi-asserted-by":"publisher","first-page":"1736","DOI":"10.1093\/humrep\/dem058","article-title":"Correlation of maternal plasma total cell-free DNA and fetal DNA levels with short term outcome of first-trimester vaginal bleeding","volume":"22","author":"Yin","year":"2007","journal-title":"Hum Reprod"},{"key":"key20240328124155_b18-BR-20-4-01757","doi-asserted-by":"publisher","first-page":"5363","DOI":"10.1080\/14767058.2021.1879043","article-title":"Fetal fraction of cell free DNA in screening for hypertensive disorders at 11-13 weeks","volume":"35","author":"Sapantzoglou","year":"2022","journal-title":"J Matern Fetal Neonatal Med"},{"key":"key20240328124155_b19-BR-20-4-01757","doi-asserted-by":"publisher","first-page":"638","DOI":"10.1016\/j.fertnstert.2003.07.028","article-title":"Cell-free fetal DNA levels in maternal plasma after elective first-trimester termination of pregnancy","volume":"81","author":"Wataganara","year":"2004","journal-title":"Fertil Steril"},{"key":"key20240328124155_b20-BR-20-4-01757","doi-asserted-by":"publisher","first-page":"e38","DOI":"10.1055\/s-0040-1721674","article-title":"Association between levels of total cell-free DNA and development of preeclampsia-A literature review","volume":"11","author":"Wu","year":"2021","journal-title":"AJP Rep"},{"key":"key20240328124155_b21-BR-20-4-01757","doi-asserted-by":"publisher","first-page":"1896S","DOI":"10.3945\/ajcn.110.000851","article-title":"Placental transport in pregnancy pathologies","volume":"94 (6 Suppl)","author":"Desoye","year":"2011","journal-title":"Am J Clin Nutr"},{"issue":"120","key":"key20240328124155_b22-BR-20-4-01757","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2350-10-120","article-title":"Relationship of circulating cell-free DNA levels to cell-free fetal DNA levels, clinical characteristics and laboratory parameters in preeclampsia","volume":"10","author":"Lazar","year":"2009","journal-title":"BMC Med Genet"},{"issue":"e021477","key":"key20240328124155_b23-BR-20-4-01757","doi-asserted-by":"publisher","DOI":"10.1161\/JAHA.121.021477","article-title":"At preeclampsia diagnosis, total cell-free DNA concentration is elevated and correlates with disease severity","volume":"10","author":"Kolarova","year":"2021","journal-title":"J Am Heart Assoc"},{"key":"key20240328124155_b24-BR-20-4-01757","doi-asserted-by":"publisher","first-page":"32","DOI":"10.1016\/j.ejogrb.2020.10.042","article-title":"Implications of non-invasive prenatal testing for identifying and managing high-risk pregnancies","volume":"256","author":"Merriel","year":"2021","journal-title":"Eur J Obstet Gynecol Reprod Biol"},{"key":"key20240328124155_b25-BR-20-4-01757","doi-asserted-by":"publisher","first-page":"643","DOI":"10.1002\/pd.4159","article-title":"SNP-based non-invasive prenatal testing detects sex chromosome aneuploidies with high accuracy","volume":"33","author":"Samango-Sprouse","year":"2013","journal-title":"Prenat Diagn"},{"key":"key20240328124155_b26-BR-20-4-01757","doi-asserted-by":"publisher","first-page":"1233","DOI":"10.1002\/pd.3993","article-title":"Noninvasive prenatal aneuploidy testing of chromosomes 13, 18, 21, X, and Y, using targeted sequencing of polymorphic loci","volume":"32","author":"Zimmermann","year":"2012","journal-title":"Prenat Diagn"},{"key":"key20240328124155_b27-BR-20-4-01757","doi-asserted-by":"publisher","first-page":"219","DOI":"10.1159\/000442931","article-title":"Validation of an enhanced version of a SNP-Based noninvasive prenatal test for detection of fetal chromosomal aneuploidies","volume":"40","author":"Ryan","year":"2016","journal-title":"Fetal Diagn Ther"},{"key":"key20240328124155_b28-BR-20-4-01757","first-page":"1323","article-title":"Maternal serum-alpha-fetoprotein measurement in antenatal screening for anencephaly and spina bifida in early pregnancy. Report of UK collaborative study on alpha-fetoprotein in relation to neural-tube defects","volume":"1","author":"Wald","year":"1977","journal-title":"Lancet"},{"key":"key20240328124155_b29-BR-20-4-01757","first-page":"425","article-title":"All MoMs are not equal: Some statistical properties associated with reporting results in the form of multiples of the median","volume":"52","author":"Bishop","year":"1993","journal-title":"Am J Hum Genet"},{"key":"key20240328124155_b30-BR-20-4-01757","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1111\/voxs.12521","article-title":"Cell-free fetal DNA and fetal blood group genotyping: Non-invasive prenatal testing","volume":"15","author":"Clausen","year":"2020","journal-title":"ISBT Science Series"},{"key":"key20240328124155_b31-BR-20-4-01757","doi-asserted-by":"publisher","first-page":"291","DOI":"10.1007\/s40291-019-00385-2","article-title":"Non-invasive prenatal testing using fetal DNA","volume":"23","author":"Breveglieri","year":"2019","journal-title":"Mol Diagn Ther"},{"key":"key20240328124155_b32-BR-20-4-01757","doi-asserted-by":"publisher","first-page":"593","DOI":"10.1002\/pd.5053","article-title":"The clinical utility of genome-wide non invasive prenatal screening","volume":"37","author":"Fiorentino","year":"2017","journal-title":"Prenat Diagn"},{"key":"key20240328124155_b33-BR-20-4-01757","author":"van der Schoot"},{"key":"key20240328124155_b34-BR-20-4-01757","first-page":"623","article-title":"Cell free fetal DNA testing in maternal blood of Romanian pregnant women","volume":"13","author":"Radoi","year":"2015","journal-title":"Iran J Reprod Med"},{"issue":"36","key":"key20240328124155_b35-BR-20-4-01757","doi-asserted-by":"publisher","DOI":"10.1186\/s13039-022-00612-2","article-title":"False-positives and false-negatives in non-invasive prenatal testing (NIPT): What can we learn from a meta-analyses on >750,000 tests?","volume":"15","author":"Liehr","year":"2022","journal-title":"Mol Cytogenet"},{"key":"key20240328124155_b36-BR-20-4-01757","doi-asserted-by":"publisher","first-page":"287","DOI":"10.5455\/msm.2020.32.287-293","article-title":"Is primary cesarean section a cause of increasing cesarean section rates in greece?","volume":"32","author":"Antoniou","year":"2020","journal-title":"Mater Sociomed"},{"key":"key20240328124155_b37-BR-20-4-01757"},{"key":"key20240328124155_b38-BR-20-4-01757","doi-asserted-by":"publisher","first-page":"778","DOI":"10.1002\/pd.407","article-title":"Decreased first trimester PAPP-A is a predictor of adverse pregnancy outcome","volume":"22","author":"Yaron","year":"2002","journal-title":"Prenat Diagn"},{"key":"key20240328124155_b39-BR-20-4-01757","doi-asserted-by":"publisher","first-page":"578","DOI":"10.1002\/pd.2246","article-title":"The impact of fetal gender on first trimester nuchal translucency and maternal serum free \u03b2-hCG and PAPP-A MoM in normal and trisomy 21 pregnancies","volume":"29","author":"Cowans","year":"2009","journal-title":"Prenat Diagn"},{"key":"key20240328124155_b40-BR-20-4-01757","doi-asserted-by":"publisher","first-page":"738","DOI":"10.1002\/1097-0223(200009)20:9<738::aid-pd917>3.0.co;2-r","article-title":"Mid-trimester \u03b2-hCG levels incorporated in a multifactorial model for the prediction of severe pre-eclampsia","volume":"20","author":"Lee","year":"2000","journal-title":"Prenat Diagn"},{"key":"key20240328124155_b41-BR-20-4-01757","doi-asserted-by":"publisher","first-page":"839","DOI":"10.1002\/pd.4141","article-title":"Association between first-trimester maternal serum pregnancy-associated plasma protein-A and obstetric complications","volume":"33","author":"D'Antonio","year":"2013","journal-title":"Prenat Diagn"},{"key":"key20240328124155_b42-BR-20-4-01757","doi-asserted-by":"publisher","first-page":"45","DOI":"10.1016\/j.ajog.2004.03.090","article-title":"Nuchal translucency and other first-trimester sonographic markers of chromosomal abnormalities","volume":"191","author":"Nicolaides","year":"2004","journal-title":"Am J Obstet Gynecol"},{"key":"key20240328124155_b43-BR-20-4-01757","doi-asserted-by":"publisher","first-page":"867","DOI":"10.1136\/bmj.304.6831.867","article-title":"Fetal nuchal translucency: Ultrasound screening for chromosomal defects in first trimester of pregnancy","volume":"304","author":"Nicolaides","year":"1992","journal-title":"BMJ"},{"key":"key20240328124155_b44-BR-20-4-01757","doi-asserted-by":"publisher","first-page":"675.e1","DOI":"10.1016\/j.ajog.2014.06.025","article-title":"Risk of selected structural abnormalities in infants after increased nuchal translucency measurement","volume":"211","author":"Baer","year":"2014","journal-title":"Am J Obstet Gynecol"},{"key":"key20240328124155_b45-BR-20-4-01757","doi-asserted-by":"publisher","first-page":"480","DOI":"10.1067\/mob.2003.27","article-title":"Cell-free fetal DNA in the plasma of pregnant women with severe fetal growth restriction","volume":"188","author":"Sekizawa","year":"2003","journal-title":"Am J Obstet Gynecol"},{"key":"key20240328124155_b46-BR-20-4-01757","doi-asserted-by":"publisher","first-page":"785","DOI":"10.1002\/pd.1504","article-title":"Correlation of fetal DNA levels in maternal plasma with Doppler status in pathological pregnancies","volume":"26","author":"Smid","year":"2006","journal-title":"Prenat Diagn"},{"key":"key20240328124155_b47-BR-20-4-01757","doi-asserted-by":"publisher","first-page":"24","DOI":"10.1159\/000236355","article-title":"Total and fetal cell-free DNA analysis in maternal blood as markers of placental insufficiency in intrauterine growth restriction","volume":"26","author":"Al Nakib","year":"2009","journal-title":"Fetal Diagn Ther"},{"key":"key20240328124155_b48-BR-20-4-01757","doi-asserted-by":"publisher","first-page":"1104","DOI":"10.1002\/pd.4940","article-title":"Absolute first trimester cell-free DNA levels and their associations with adverse pregnancy outcomes","volume":"36","author":"Thurik","year":"2016","journal-title":"Prenat Diagn"},{"key":"key20240328124155_b49-BR-20-4-01757","doi-asserted-by":"publisher","first-page":"312","DOI":"10.1373\/clinchem.2004.042713","article-title":"Accurate and robust quantification of circulating fetal and total DNA in maternal plasma from 5 to 41 weeks of gestation","volume":"51","author":"Birch","year":"2005","journal-title":"Clin Chem"},{"key":"key20240328124155_b50-BR-20-4-01757","doi-asserted-by":"publisher","first-page":"1611","DOI":"10.1016\/j.cca.2010.07.032","article-title":"Cell-free DNA in the blood as a solid tumor biomarker-a critical appraisal of the literature","volume":"411","author":"Jung","year":"2010","journal-title":"Clin Chim Acta"},{"key":"key20240328124155_b51-BR-20-4-01757","doi-asserted-by":"publisher","first-page":"418","DOI":"10.1002\/pd.2484","article-title":"A new methodology to preserve the original proportion and integrity of cell-free fetal DNA in maternal plasma during sample processing and storage","volume":"30","author":"Fernando","year":"2010","journal-title":"Prenat Diagn"},{"issue":"e101500","key":"key20240328124155_b52-BR-20-4-01757","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0101500","article-title":"Quantification of cell-free DNA in normal and complicated pregnancies: Overcoming biological and technical issues","volume":"9","author":"Manokhina","year":"2014","journal-title":"PLoS One"},{"key":"key20240328124155_b53-BR-20-4-01757","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1007\/s40291-016-0245-9","article-title":"Cell-Free Fetal DNA for the prediction of pre-eclampsia at the first and second trimesters: A systematic review and meta-analysis","volume":"21","author":"Contro","year":"2017","journal-title":"Mol Diagn Ther"},{"key":"key20240328124155_b54-BR-20-4-01757","doi-asserted-by":"publisher","first-page":"55","DOI":"10.5603\/GP.2019.0009","article-title":"Is there a future for cell-free fetal dna tests in screening for preeclampsia?","volume":"90","author":"Sarzynska-Nowacka","year":"2019","journal-title":"Ginekol Pol"},{"key":"key20240328124155_b55-BR-20-4-01757","doi-asserted-by":"publisher","first-page":"215","DOI":"10.1159\/000346806","article-title":"Maternal plasma cell-free fetal and maternal DNA at 11-13 weeks' gestation: Relation to fetal and maternal characteristics and pregnancy outcomes","volume":"33","author":"Poon","year":"2013","journal-title":"Fetal Diagn Ther"},{"key":"key20240328124155_b56-BR-20-4-01757","doi-asserted-by":"publisher","first-page":"722","DOI":"10.1002\/uog.18993","article-title":"Association between fetal fraction on cell-free DNA testing and first-trimester markers for pre-eclampsia","volume":"52","author":"Rolnik","year":"2018","journal-title":"Ultrasound Obstet Gynecol"},{"key":"key20240328124155_b57-BR-20-4-01757","doi-asserted-by":"publisher","first-page":"101","DOI":"10.1002\/uog.14666","article-title":"Fetal fraction of cell-free DNA in maternal plasma in the prediction of spontaneous preterm delivery","volume":"45","author":"Quezada","year":"2015","journal-title":"Ultrasound Obstet Gynecol"},{"issue":"e1787","key":"key20240328124155_b58-BR-20-4-01757","doi-asserted-by":"publisher","DOI":"10.1002\/mgg3.1787","article-title":"A two-year prospective study assessing the performance of fetal chromosomal microarray analysis and next-generation sequencing in high-risk pregnancies","volume":"9","author":"Ridn\u00f5i","year":"2021","journal-title":"Mol Genet Genomic Med"}],"container-title":["Biomedical Reports"],"original-title":[],"deposited":{"date-parts":[[2024,3,28]],"date-time":"2024-03-28T10:42:03Z","timestamp":1711622523000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.spandidos-publications.com\/10.3892\/br.2024.1757"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,3,4]]},"references-count":58,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2024,3,4]]}},"URL":"https:\/\/doi.org\/10.3892\/br.2024.1757","relation":{},"ISSN":["2049-9434","2049-9442"],"issn-type":[{"value":"2049-9434","type":"print"},{"value":"2049-9442","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,3,4]]},"article-number":"69"}}