{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T13:07:14Z","timestamp":1776258434267,"version":"3.50.1"},"reference-count":45,"publisher":"Spandidos Publications","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,5]]},"DOI":"10.3892\/ijo.2017.3935","type":"journal-article","created":{"date-parts":[[2017,3,27]],"date-time":"2017-03-27T06:04:15Z","timestamp":1490594655000},"page":"1579-1589","source":"Crossref","is-referenced-by-count":17,"title":["Routine genetic testing of lung cancer specimens derived from surgery, bronchoscopy and fluid aspiration by next generation sequencing"],"prefix":"10.3892","volume":"50","author":[{"given":"Gou","family":"Yamamoto","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mari","family":"Kikuchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shiho","family":"Kobayashi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshiko","family":"Arai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenji","family":"Fujiyoshi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomokazu","family":"Wakatsuki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miho","family":"Kakuta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuki","family":"Yamane","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshihito","family":"Iijima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hideaki","family":"Mizutani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuki","family":"Nakajima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junko","family":"Sudo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroyasu","family":"Kinoshita","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Futoshi","family":"Kurimoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hirohiko","family":"Akiyama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hidetaka","family":"Uramoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshi","family":"Sakai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshito","family":"Akagi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kiwamu","family":"Akagi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2249","published-online":{"date-parts":[[2017,3,27]]},"reference":[{"key":"key20180107175850_b1-ijo-50-05-1579","doi-asserted-by":"crossref","first-page":"7","DOI":"10.3322\/caac.21332","article-title":"Cancer statistics, 2016","volume":"66","author":"Siegel","year":"2016","journal-title":"CA Cancer J Clin"},{"key":"key20180107175850_b2-ijo-50-05-1579","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1007\/s10147-011-0262-y","article-title":"Lung cancers unrelated to smoking: Characterized by single oncogene addiction?","volume":"16","author":"Suda","year":"2011","journal-title":"Int J Clin Oncol"},{"key":"key20180107175850_b3-ijo-50-05-1579","doi-asserted-by":"crossref","first-page":"1497","DOI":"10.1126\/science.1099314","article-title":"EGFR mutations in lung cancer: Correlation with clinical response to gefitinib therapy","volume":"304","author":"Paez","year":"2004","journal-title":"Science"},{"key":"key20180107175850_b4-ijo-50-05-1579","doi-asserted-by":"crossref","first-page":"2129","DOI":"10.1056\/NEJMoa040938","article-title":"Activating mutations in the epidermal growth factor receptor underlying responsiveness of non-small-cell lung cancer to gefitinib","volume":"350","author":"Lynch","year":"2004","journal-title":"N Engl J Med"},{"key":"key20180107175850_b5-ijo-50-05-1579","doi-asserted-by":"crossref","first-page":"2866","DOI":"10.1200\/JCO.2010.33.4235","article-title":"Biomarker analyses and final overall survival results from a phase III, randomized, open-label, first-line study of gefitinib versus carboplatin\/paclitaxel in clinically selected patients with advanced non-small-cell lung cancer in Asia (IPASS)","volume":"29","author":"Fukuoka","year":"2011","journal-title":"J Clin Oncol"},{"key":"key20180107175850_b6-ijo-50-05-1579","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1038\/nature05945","article-title":"Identification of the transforming EML4-ALK fusion gene in non-small-cell lung cancer","volume":"448","author":"Soda","year":"2007","journal-title":"Nature"},{"key":"key20180107175850_b7-ijo-50-05-1579","doi-asserted-by":"crossref","first-page":"1693","DOI":"10.1056\/NEJMoa1006448","article-title":"Anaplastic lymphoma kinase inhibition in non-small-cell lung cancer","volume":"363","author":"Kwak","year":"2010","journal-title":"N Engl J Med"},{"key":"key20180107175850_b8-ijo-50-05-1579","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1038\/nm.2658","article-title":"RET, ROS1 and ALK fusions in lung cancer","volume":"18","author":"Takeuchi","year":"2012","journal-title":"Nat Med"},{"key":"key20180107175850_b9-ijo-50-05-1579","doi-asserted-by":"crossref","first-page":"e141","DOI":"10.1200\/JCO.2013.50.5016","article-title":"Effect of the RET inhibitor vandetanib in a patient with RET fusion-positive metastatic non-Small-cell Lung cancer","volume":"34","author":"Falchook","year":"2016","journal-title":"J Clin Oncol"},{"key":"key20180107175850_b10-ijo-50-05-1579","doi-asserted-by":"crossref","first-page":"1963","DOI":"10.1056\/NEJMoa1406766","article-title":"Crizotinib in ROS1-rearranged non-small-cell lung cancer","volume":"371","author":"Shaw","year":"2014","journal-title":"N Engl J Med"},{"key":"key20180107175850_b11-ijo-50-05-1579","doi-asserted-by":"crossref","first-page":"1039","DOI":"10.1200\/JCO.2012.45.3753","article-title":"Genotyping and genomic profiling of non-small-cell lung cancer: Implications for current and future therapies","volume":"31","author":"Li","year":"2013","journal-title":"J Clin Oncol"},{"key":"key20180107175850_b12-ijo-50-05-1579","doi-asserted-by":"crossref","first-page":"2176","DOI":"10.3892\/ol.2015.3600","article-title":"Determination of EGFR and KRAS mutational status in Greek non-small-cell lung cancer patients","volume":"10","author":"Papadopoulou","year":"2015","journal-title":"Oncol Lett"},{"key":"key20180107175850_b13-ijo-50-05-1579","doi-asserted-by":"crossref","first-page":"2477","DOI":"10.1093\/annonc\/mdv475","article-title":"Clinical application of amplicon-based next-generation sequencing to therapeutic decision making in lung cancer","volume":"26","author":"Takeda","year":"2015","journal-title":"Ann Oncol"},{"key":"key20180107175850_b14-ijo-50-05-1579","doi-asserted-by":"crossref","first-page":"22338","DOI":"10.1038\/srep22338","article-title":"A targeted next-generation sequencing method for identifying clinically relevant mutation profiles in lung adenocarcinoma","volume":"6","author":"Shao","year":"2016","journal-title":"Sci Rep"},{"key":"key20180107175850_b15-ijo-50-05-1579","doi-asserted-by":"crossref","first-page":"e107","DOI":"10.1093\/nar\/gku489","article-title":"Integrated RNA and DNA sequencing improves mutation detection in low purity tumors","volume":"42","author":"Wilkerson","year":"2014","journal-title":"Nucleic Acids Res"},{"key":"key20180107175850_b16-ijo-50-05-1579","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1007\/s11523-007-0053-6","article-title":"Simple method to detect important epidermal growth factor receptor gene mutations with bronchoscopic specimens of lung cancer patients for gefitinib treatment","volume":"2","author":"Akagi","year":"2007","journal-title":"Target Oncol"},{"key":"key20180107175850_b17-ijo-50-05-1579","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1038\/jhg.2016.12","article-title":"Human genetic variation database, a reference database of genetic variations in the Japanese population","volume":"61","author":"Higasa","year":"2016","journal-title":"J Hum Genet"},{"key":"key20180107175850_b18-ijo-50-05-1579","doi-asserted-by":"crossref","first-page":"8018","DOI":"10.1038\/ncomms9018","article-title":"Rare variant discovery by deep whole-genome sequencing of 1,070 Japanese individuals","volume":"6","author":"Nagasaki","year":"2015","journal-title":"Nat Commun"},{"key":"key20180107175850_b19-ijo-50-05-1579","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1158\/2159-8290.CD-12-0095","article-title":"The cBio cancer genomics portal: An open platform for exploring multidimensional cancer genomics data","volume":"2","author":"Cerami","year":"2012","journal-title":"Cancer Discov"},{"key":"key20180107175850_b20-ijo-50-05-1579","doi-asserted-by":"crossref","first-page":"pl1","DOI":"10.1126\/scisignal.2004088","article-title":"Integrative analysis of complex cancer genomics and clinical profiles using the cBio-Portal","volume":"6","author":"Gao","year":"2013","journal-title":"Sci Signal"},{"key":"key20180107175850_b21-ijo-50-05-1579","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1016\/j.lungcan.2014.11.013","article-title":"Efficacy of EGFR tyrosine kinase inhibitors in patients with EGFR-mutated non-small cell lung cancer except both exon 19 deletion and exon 21 L858R: A retrospective analysis in Korea","volume":"87","author":"Baek","year":"2015","journal-title":"Lung Cancer"},{"key":"key20180107175850_b22-ijo-50-05-1579","doi-asserted-by":"crossref","first-page":"1276","DOI":"10.1634\/theoncologist.2008-0093","article-title":"Good response to gefitinib in lung adeno-carcinoma of complex epidermal growth factor receptor (EGFR) mutations with the classical mutation pattern","volume":"13","author":"Wu","year":"2008","journal-title":"Oncologist"},{"key":"key20180107175850_b23-ijo-50-05-1579","doi-asserted-by":"crossref","first-page":"e468","DOI":"10.1200\/JCO.2010.33.5802","article-title":"Good response to gefitinib in a lung adenocarcinoma harboring a heterozygous complex mutation of L833V and H835L in epidermal growth factor receptor gene","volume":"29","author":"Yang","year":"2011","journal-title":"J Clin Oncol"},{"key":"key20180107175850_b24-ijo-50-05-1579","doi-asserted-by":"crossref","first-page":"1182","DOI":"10.1200\/JCO.2007.14.9039","article-title":"Differential responses to erlotinib in epidermal growth factor receptor (EGFR)-mutated lung cancers with acquired resistance to gefitinib carrying the L747S or T790M secondary mutations","volume":"26","author":"Costa","year":"2008","journal-title":"J Clin Oncol"},{"key":"key20180107175850_b25-ijo-50-05-1579","doi-asserted-by":"crossref","first-page":"8919","DOI":"10.1158\/0008-5472.CAN-04-2818","article-title":"Mutations of the epidermal growth factor receptor gene in lung cancer: Biological and clinical implications","volume":"64","author":"Kosaka","year":"2004","journal-title":"Cancer Res"},{"key":"key20180107175850_b26-ijo-50-05-1579","doi-asserted-by":"crossref","first-page":"e73484","DOI":"10.1371\/journal.pone.0073484","article-title":"Identification and characterization of cancer mutations in Japanese lung adenocarcinoma without sequencing of normal tissue counterparts","volume":"8","author":"Suzuki","year":"2013","journal-title":"PLoS One"},{"key":"key20180107175850_b27-ijo-50-05-1579","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/S1470-2045(12)70489-8","article-title":"Selumetinib plus docetaxel for KRAS-mutant advanced non-small-cell lung cancer: A randomised, multicentre, placebo-controlled, phase 2 study","volume":"14","author":"J\u00e4nne","year":"2013","journal-title":"Lancet Oncol"},{"key":"key20180107175850_b28-ijo-50-05-1579","doi-asserted-by":"crossref","first-page":"3812","DOI":"10.1158\/1078-0432.CCR-10-3408","article-title":"Effectiveness of tyrosine kinase inhibitors on 'uncommon' epidermal growth factor receptor mutations of unknown clinical significance in non-small cell lung cancer","volume":"17","author":"Wu","year":"2011","journal-title":"Clin Cancer Res"},{"key":"key20180107175850_b29-ijo-50-05-1579","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1016\/j.jtho.2015.12.107","article-title":"Examining treatment outcomes with erlotinib in patients with advanced non-small cell lung cancer whose tumors harbor uncommon EGFR mutations","volume":"11","author":"Klughammer","year":"2016","journal-title":"J Thorac Oncol"},{"key":"key20180107175850_b30-ijo-50-05-1579","doi-asserted-by":"crossref","first-page":"7519","DOI":"10.1158\/1078-0432.CCR-08-0151","article-title":"Acquired resistance to epidermal growth factor receptor kinase inhibitors associated with a novel T854A mutation in a patient with EGFR-mutant lung adenocarcinoma","volume":"14","author":"Bean","year":"2008","journal-title":"Clin Cancer Res"},{"key":"key20180107175850_b31-ijo-50-05-1579","doi-asserted-by":"crossref","first-page":"90ra59","DOI":"10.1126\/scitranslmed.3002356","article-title":"Optimization of dosing for EGFR-mutant non-small cell lung cancer with evolutionary cancer modeling","volume":"3","author":"Chmielecki","year":"2011","journal-title":"Sci Transl Med"},{"key":"key20180107175850_b32-ijo-50-05-1579","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1038\/nm.4040","article-title":"Tumor cells can follow distinct evolutionary paths to become resistant to epidermal growth factor receptor inhibition","volume":"22","author":"Hata","year":"2016","journal-title":"Nat Med"},{"key":"key20180107175850_b33-ijo-50-05-1579","doi-asserted-by":"crossref","first-page":"560","DOI":"10.1038\/nm.3854","article-title":"Acquired EGFR C797S mutation mediates resistance to AZD9291 in non-small cell lung cancer harboring EGFR T790M","volume":"21","author":"Thress","year":"2015","journal-title":"Nat Med"},{"key":"key20180107175850_b34-ijo-50-05-1579","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1183\/16000617.0071-2015","article-title":"KRAS oncogene in lung cancer: Focus on molecularly driven clinical trials","volume":"25","author":"Kempf","year":"2016","journal-title":"Eur Respir Rev"},{"key":"key20180107175850_b35-ijo-50-05-1579","doi-asserted-by":"crossref","first-page":"548","DOI":"10.1038\/nature12796","article-title":"K-Ras(G12C) inhibitors allosterically control GTP affinity and effector interactions","volume":"503","author":"Ostrem","year":"2013","journal-title":"Nature"},{"key":"key20180107175850_b36-ijo-50-05-1579","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.lungcan.2015.11.004","article-title":"Correlation between KRAS mutation status and response to chemotherapy in patients with advanced non-small cell lung cancer","volume":"92","author":"Hames","year":"2016","journal-title":"Lung Cancer"},{"key":"key20180107175850_b37-ijo-50-05-1579","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/S1470-2045(10)70087-5","article-title":"New driver mutations in non-small-cell lung cancer","volume":"12","author":"Pao","year":"2011","journal-title":"Lancet Oncol"},{"key":"key20180107175850_b38-ijo-50-05-1579","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/j.lungcan.2014.05.018","article-title":"CRKL amplification is rare as a mechanism for acquired resistance to kinase inhibitors in lung cancers with epidermal growth factor receptor mutation","volume":"85","author":"Suda","year":"2014","journal-title":"Lung Cancer"},{"key":"key20180107175850_b39-ijo-50-05-1579","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.lungcan.2015.01.023","article-title":"Multiple resistant factors in lung cancer with primary resistance to EGFR-TK inhibitors confer poor survival","volume":"88","author":"Kim","year":"2015","journal-title":"Lung Cancer"},{"key":"key20180107175850_b40-ijo-50-05-1579","first-page":"209ra153","article-title":"A genomics-based classification of human lung tumors","volume":"5","author":"Clinical Lung Cancer Genome Project (CLCGP)","year":"2013","journal-title":"Sci Transl Med"},{"key":"key20180107175850_b41-ijo-50-05-1579","doi-asserted-by":"crossref","first-page":"1415","DOI":"10.1016\/S0140-6736(16)00004-0","article-title":"Routine molecular profiling of patients with advanced non-small-cell lung cancer: Results of a 1-year nationwide programme of the French Cooperative Thoracic Intergroup (IFCT)","volume":"387","author":"Barlesi","year":"2016","journal-title":"Lancet"},{"key":"key20180107175850_b42-ijo-50-05-1579","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1158\/2159-8290.CD-NB2016-018","article-title":"NCI-MATCH trial draws strong interest","volume":"6","author":"Colwell","year":"2016","journal-title":"Cancer Discov"},{"key":"key20180107175850_b43-ijo-50-05-1579","doi-asserted-by":"crossref","first-page":"82943","DOI":"10.18632\/oncotarget.12705","article-title":"ALK FISH patterns and the detection of ALK fusions by next generation sequencing in lung adenocarcinoma","volume":"7","author":"Dacic","year":"2016","journal-title":"Oncotarget"},{"key":"key20180107175850_b44-ijo-50-05-1579","article-title":"Osimertinib or platinum-pemetrexed in EGFR T790M-positive lung cancer","author":"Mok","year":"2016","journal-title":"N Engl J Med"},{"key":"key20180107175850_b45-ijo-50-05-1579","doi-asserted-by":"crossref","first-page":"915","DOI":"10.1158\/1078-0432.CCR-15-1627-T","article-title":"Bias-corrected targeted next-generation sequencing for rapid, multiplexed detection of actionable alterations in cell-free DNA from advanced lung cancer patients","volume":"22","author":"Paweletz","year":"2016","journal-title":"Clin Cancer Res"}],"container-title":["International Journal of Oncology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/spandidos-publications.com\/10.3892\/ijo.2017.3935\/download","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,10,4]],"date-time":"2020-10-04T11:28:38Z","timestamp":1601810918000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.spandidos-publications.com\/10.3892\/ijo.2017.3935"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,3,27]]},"references-count":45,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2017,3,27]]},"published-print":{"date-parts":[[2017,5]]}},"alternative-id":["10.3892\/ijo.2017.3935"],"URL":"https:\/\/doi.org\/10.3892\/ijo.2017.3935","relation":{},"ISSN":["1019-6439","1791-2423"],"issn-type":[{"value":"1019-6439","type":"print"},{"value":"1791-2423","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,3,27]]}}}