{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,25]],"date-time":"2026-10-25T05:38:20Z","timestamp":1792906700031,"version":"4.5.0"},"reference-count":47,"publisher":"Spandidos Publications","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mol Med Rep"],"DOI":"10.3892\/mmr.2026.14042","type":"journal-article","created":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T11:54:17Z","timestamp":1791892457000},"page":"1-19","source":"Crossref","is-referenced-by-count":0,"title":["Piezo1 knockdown alleviates high\u2011altitude cerebral edema by improving brain microvascular endothelial cell tight junctions via blocking the calcium influx\u2011mediated Calpain\u20111 activity"],"prefix":"10.3892","volume":"34","author":[{"given":"Taijian","family":"Cao","sequence":"first","affiliation":[{"name":"Department of Spinal Surgery, Qinghai University Affiliated Hospital, College of Clinical Medicine, Qinghai University, Xining, Qinghai 810001, P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhicheng","family":"Xiang","sequence":"additional","affiliation":[{"name":"College of Clinical Medicine, Qinghai University, Xining, Qinghai 810001, P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yangyang","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Plastic Surgery, Qinghai University Affiliated Hospital, College of Clinical Medicine, Qinghai University, Xining, Qinghai 810001, P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Drolma","family":"Gomchok","sequence":"additional","affiliation":[{"name":"College of Clinical Medicine, Qinghai University, Xining, Qinghai 810001, P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haifeng","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Qinghai Provincial People's Hospital, Xining, Qinghai 810006, P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiwang","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, High\u2011Altitude Disease Clinical Medical Research Center of Qinghai Province, Qinghai Provincial People's Hospital, Xining, Qinghai 810006, P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dahai","family":"Cao","sequence":"additional","affiliation":[{"name":"College of Clinical Medicine, Qinghai University, Xining, Qinghai 810001, P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunqing","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Clinical Medicine, Qinghai University, Xining, Qinghai 810001, P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, High\u2011Altitude Disease Clinical Medical Research Center of Qinghai Province, Qinghai Provincial People's Hospital, Xining, Qinghai 810006, P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2249","published-online":{"date-parts":[[2026,10,13]]},"reference":[{"key":"key20261025073152_b1-mmr-34-6-14042","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1038\/s41572-024-00526-w","article-title":"Altitude illnesses","volume":"10","author":"Gatterer","year":"2024","journal-title":"Nat Rev Dis Primers"},{"key":"key20261025073152_b2-mmr-34-6-14042","doi-asserted-by":"publisher","first-page":"1902","DOI":"10.1152\/jn.00381.2024","article-title":"High-altitude-induced cerebral edema in mice is alleviated by bestatin-mediated blood-brain barrier protection","volume":"133","author":"Wang","year":"2025","journal-title":"J Neurophysiol"},{"key":"key20261025073152_b3-mmr-34-6-14042","doi-asserted-by":"publisher","first-page":"160","DOI":"10.1186\/s12964-022-00976-3","article-title":"Caveolin-1 accelerates hypoxia-induced endothelial dysfunction in high-altitude cerebral edema","volume":"20","author":"Xue","year":"2022","journal-title":"Cell Commun Signal"},{"key":"key20261025073152_b4-mmr-34-6-14042","doi-asserted-by":"publisher","first-page":"404","DOI":"10.2174\/011570159X371235250417051313","article-title":"Hydroxychloroquine prevents high-altitude cerebral edema by inhibiting endothelial claudin-5 autophagic degradation","volume":"24","author":"Xue","year":"2026","journal-title":"Curr Neuropharmacol"},{"key":"key20261025073152_b5-mmr-34-6-14042","doi-asserted-by":"publisher","first-page":"113873","DOI":"10.1016\/j.yexcr.2023.113873","article-title":"Acute brain injury increases pulmonary capillary permeability via sympathetic activation-mediated high fluid shear stress and destruction of the endothelial glycocalyx layer","volume":"434","author":"Zhao","year":"2024","journal-title":"Exp Cell Res"},{"key":"key20261025073152_b6-mmr-34-6-14042","first-page":"2710","article-title":"Aging increases hypoxia-induced endothelial permeability and blood-brain barrier dysfunction by upregulating arginase-II","volume":"15","author":"Cheng","year":"2024","journal-title":"Aging Dis"},{"key":"key20261025073152_b7-mmr-34-6-14042","doi-asserted-by":"publisher","first-page":"103346","DOI":"10.1016\/j.redox.2024.103346","article-title":"LOX-mediated ECM mechanical stress induces Piezo1 activation in hypoxic-ischemic brain damage and identification of novel inhibitor of LOX","volume":"76","author":"Jiang","year":"2024","journal-title":"Redox Biol"},{"key":"key20261025073152_b8-mmr-34-6-14042","doi-asserted-by":"publisher","first-page":"105702","DOI":"10.1016\/j.neuint.2024.105702","article-title":"Inhibition of piezo1 prevents chronic cerebral hypoperfusion-induced cognitive impairment and blood brain barrier disruption","volume":"175","author":"Xu","year":"2024","journal-title":"Neurochem Int"},{"key":"key20261025073152_b9-mmr-34-6-14042","doi-asserted-by":"publisher","first-page":"259","DOI":"10.1007\/s00018-025-05804-8","article-title":"Piezo1 disrupts blood-brain barrier via CaMKII\/Nrf2 in ischemic stroke","volume":"82","author":"Fu","year":"2025","journal-title":"Cell Mol Life Sci"},{"key":"key20261025073152_b10-mmr-34-6-14042","doi-asserted-by":"publisher","first-page":"215","DOI":"10.1385\/MN:33:3:215","article-title":"Calpain and synaptic function","volume":"33","author":"Wu","year":"2006","journal-title":"Mol Neurobiol"},{"key":"key20261025073152_b11-mmr-34-6-14042","doi-asserted-by":"publisher","first-page":"725274","DOI":"10.3389\/fmolb.2021.725274","article-title":"PIEZO1 Ion channel mediates ionizing radiation-induced pulmonary endothelial cell ferroptosis via Ca2+\/calpain\/VE-cadherin signaling","volume":"8","author":"Guo","year":"2021","journal-title":"Front Mol Biosci"},{"key":"key20261025073152_b12-mmr-34-6-14042","doi-asserted-by":"publisher","first-page":"3238","DOI":"10.1161\/STROKEAHA.111.615559","article-title":"Increased blood-brain barrier permeability and brain edema after focal cerebral ischemia induced by hyperlipidemia: Role of lipid peroxidation and calpain-1\/2, matrix metalloproteinase-2\/9, and RhoA overactivation","volume":"42","author":"ElAli","year":"2011","journal-title":"Stroke"},{"key":"key20261025073152_b13-mmr-34-6-14042","doi-asserted-by":"publisher","first-page":"26958","DOI":"10.1074\/jbc.M116.735365","article-title":"Attenuation of blood-brain barrier breakdown and hyperpermeability by calpain inhibition","volume":"291","author":"Alluri","year":"2016","journal-title":"J Biol Chem"},{"key":"key20261025073152_b14-mmr-34-6-14042","doi-asserted-by":"publisher","first-page":"289","DOI":"10.4103\/1673-5374.244794","article-title":"Structural and functional damage to the hippocampal neurovascular unit in diabetes-related depression","volume":"14","author":"Liu","year":"2019","journal-title":"Neural Regen Res"},{"key":"key20261025073152_b15-mmr-34-6-14042","doi-asserted-by":"publisher","first-page":"H980","DOI":"10.1152\/ajpheart.00597.2020","article-title":"Endothelial function and shear stress in hypobaric hypoxia: Time course and impact of plasma volume expansion in men","volume":"319","author":"Tremblay","year":"2020","journal-title":"Am J Physiol Heart Circ Physiol"},{"key":"key20261025073152_b16-mmr-34-6-14042","doi-asserted-by":"publisher","first-page":"5759","DOI":"10.1113\/JP277191","article-title":"The impact of hypoxaemia on vascular function in lowlanders and high altitude indigenous populations","volume":"597","author":"Tymko","year":"2019","journal-title":"J Physiol"},{"key":"key20261025073152_b17-mmr-34-6-14042","doi-asserted-by":"publisher","first-page":"22","DOI":"10.1186\/s11658-023-00436-x","article-title":"Oscillatory shear stress modulates Notch-mediated endothelial mesenchymal plasticity in cerebral arteriovenous malformations","volume":"28","author":"Karthika","year":"2023","journal-title":"Cell Mol Biol Lett"},{"key":"key20261025073152_b18-mmr-34-6-14042","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1186\/s12987-022-00344-w","article-title":"Exposure of human cerebral microvascular endothelial cells hCMEC\/D3 to laminar shear stress induces vascular protective responses","volume":"19","author":"Choublier","year":"2022","journal-title":"Fluids Barriers CNS"},{"key":"key20261025073152_b19-mmr-34-6-14042","doi-asserted-by":"publisher","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2(\u2212Delta Delta C(T)) method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"key20261025073152_b20-mmr-34-6-14042","doi-asserted-by":"publisher","first-page":"949412","DOI":"10.3389\/fncel.2022.949412","article-title":"Simple and efficient protocol to isolate and culture brain microvascular endothelial cells from newborn mice","volume":"16","author":"Nicolicht-Amorim","year":"2022","journal-title":"Front Cell Neurosci"},{"key":"key20261025073152_b21-mmr-34-6-14042","doi-asserted-by":"publisher","first-page":"e0341743","DOI":"10.1371\/journal.pone.0341743","article-title":"Recurrent intermittent hyponatremia: A new experimental model","volume":"21","author":"Tejedor","year":"2026","journal-title":"PLoS One"},{"key":"key20261025073152_b22-mmr-34-6-14042","doi-asserted-by":"publisher","first-page":"109896","DOI":"10.1016\/j.neuropharm.2024.109896","article-title":"Roles of mechanosensitive ion channel PIEZO1 in the pathogenesis of brain injury after experimental intracerebral hemorrhage","volume":"251","author":"Qi","year":"2024","journal-title":"Neuropharmacology"},{"key":"key20261025073152_b23-mmr-34-6-14042","first-page":"1750","article-title":"Piezo1 suppression reduces demyelination after intracerebral hemorrhage","volume":"18","author":"Qu","year":"2023","journal-title":"Neural Regen Res"},{"key":"key20261025073152_b24-mmr-34-6-14042","doi-asserted-by":"publisher","first-page":"110327","DOI":"10.1016\/j.mehy.2020.110327","article-title":"PIEZO1 might be involved in cerebral ischemia-reperfusion injury through ferroptosis regulation: A hypothesis","volume":"146","author":"Guo","year":"2021","journal-title":"Med Hypotheses"},{"key":"key20261025073152_b25-mmr-34-6-14042","doi-asserted-by":"publisher","first-page":"263","DOI":"10.3390\/diseases13080263","article-title":"PIEZO channels in mechano-inflammation: Gatekeepers of neuroimmune crosstalk","volume":"13","author":"Pirri","year":"2025","journal-title":"Diseases"},{"key":"key20261025073152_b26-mmr-34-6-14042","doi-asserted-by":"publisher","first-page":"237","DOI":"10.1186\/s12974-026-03843-x","article-title":"Piezo1 in the central nervous system: Decoding the mechanical signature of neuroinflammation","volume":"23","author":"Shi","year":"2026","journal-title":"J Neuroinflammation"},{"key":"key20261025073152_b27-mmr-34-6-14042","doi-asserted-by":"publisher","first-page":"106562","DOI":"10.1016\/j.neubiorev.2026.106562","article-title":"Insights into the role of the mechanosensitive Piezo1 channel and signaling mechanisms in CNS functions and diseases","volume":"183","author":"Zhang","year":"2026","journal-title":"Neurosci Biobehav Rev"},{"key":"key20261025073152_b28-mmr-34-6-14042","doi-asserted-by":"publisher","first-page":"147987","DOI":"10.1016\/j.brainres.2022.147987","article-title":"Dynamic cerebral blood flow changes with FOXOs stimulation are involved in neuronal damage associated with high-altitude cerebral edema in mice","volume":"1790","author":"Shi","year":"2022","journal-title":"Brain Res"},{"key":"key20261025073152_b29-mmr-34-6-14042","doi-asserted-by":"publisher","first-page":"198","DOI":"10.1038\/sj.jcbfm.9600513","article-title":"Hypoxia-induced acute mountain sickness is associated with intracellular cerebral edema: A 3 T magnetic resonance imaging study","volume":"28","author":"Schoonman","year":"2008","journal-title":"J Cereb Blood Flow Metab"},{"key":"key20261025073152_b30-mmr-34-6-14042","doi-asserted-by":"publisher","first-page":"372","DOI":"10.1021\/acsptsci.0c00217","article-title":"Extending the calpain-cathepsin hypothesis to the neurovasculature: Protection of brain endothelial cells and mice from neurotrauma","volume":"4","author":"Knopp","year":"2021","journal-title":"ACS Pharmacol Transl Sci"},{"key":"key20261025073152_b31-mmr-34-6-14042","doi-asserted-by":"publisher","first-page":"87997","DOI":"10.18632\/oncotarget.21324","article-title":"Postnatal calpain inhibition elicits cerebellar cell death and motor dysfunction","volume":"8","author":"Li","year":"2017","journal-title":"Oncotarget"},{"key":"key20261025073152_b32-mmr-34-6-14042","doi-asserted-by":"publisher","first-page":"e2405705","DOI":"10.1002\/advs.202405705","article-title":"Piezo1 regulates stiffness-dependent DRG axon regeneration via modifying cytoskeletal dynamics","volume":"11","author":"Lei","year":"2024","journal-title":"Adv Sci (Weinh)"},{"key":"key20261025073152_b33-mmr-34-6-14042","doi-asserted-by":"publisher","first-page":"14267","DOI":"10.1038\/s41598-018-32572-2","article-title":"Enantiomeric A\u03b2 peptides inhibit the fluid shear stress response of PIEZO1","volume":"8","author":"Maneshi","year":"2018","journal-title":"Sci Rep"},{"key":"key20261025073152_b34-mmr-34-6-14042","doi-asserted-by":"publisher","first-page":"309","DOI":"10.1016\/bs.ctm.2016.11.005","article-title":"A microfluidic approach for studying piezo channels","volume":"79","author":"Maneshi","year":"2017","journal-title":"Curr Top Membr"},{"key":"key20261025073152_b35-mmr-34-6-14042","doi-asserted-by":"publisher","first-page":"100171","DOI":"10.1074\/jbc.RA120.015059","article-title":"Piezo1 acts upstream of TRPV4 to induce pathological changes in endothelial cells due to shear stress","volume":"296","author":"Swain","year":"2021","journal-title":"J Biol Chem"},{"key":"key20261025073152_b36-mmr-34-6-14042","doi-asserted-by":"publisher","first-page":"147","DOI":"10.1016\/j.bbrc.2019.03.163","article-title":"Piezo1 mediates neuron oxygen-glucose deprivation\/reoxygenation injury via Ca2+\/calpain signaling","volume":"513","author":"Wang","year":"2019","journal-title":"Biochem Biophys Res Commun"},{"key":"key20261025073152_b37-mmr-34-6-14042","doi-asserted-by":"publisher","first-page":"113594","DOI":"10.1016\/j.bbr.2021.113594","article-title":"Inhibition of Piezo1\/Ca2+\/calpain signaling in the rat basal forebrain reverses sleep deprivation-induced fear memory impairments","volume":"417","author":"Ma","year":"2022","journal-title":"Behav Brain Res"},{"key":"key20261025073152_b38-mmr-34-6-14042","doi-asserted-by":"publisher","first-page":"117473","DOI":"10.1016\/j.atherosclerosis.2024.117473","article-title":"Low shear stress exacerbates atherosclerosis by inducing the generation of neutrophil extracellular traps via Piezo1-mediated mechanosensation","volume":"391","author":"Zhu","year":"2024","journal-title":"Atherosclerosis"},{"key":"key20261025073152_b39-mmr-34-6-14042","doi-asserted-by":"publisher","first-page":"1641","DOI":"10.1016\/j.neuron.2022.02.017","article-title":"Pericyte-to-endothelial cell signaling via vitronectin-integrin regulates blood-CNS barrier","volume":"110","author":"Ayloo","year":"2022","journal-title":"Neuron"},{"key":"key20261025073152_b40-mmr-34-6-14042","doi-asserted-by":"publisher","first-page":"5327","DOI":"10.1021\/acsbiomaterials.3c01898","article-title":"Sensitivity and validation of porous membrane electrical cell substrate impedance spectroscopy (PM-ECIS) for measuring endothelial barrier properties","volume":"10","author":"Ugodnikov","year":"2024","journal-title":"ACS Biomater Sci Eng"},{"key":"key20261025073152_b41-mmr-34-6-14042","doi-asserted-by":"publisher","first-page":"e0152105","DOI":"10.1371\/journal.pone.0152105","article-title":"Human brain microvascular endothelial cells derived from the BC1 iPS cell line exhibit a blood-brain barrier phenotype","volume":"11","author":"Katt","year":"2016","journal-title":"PLoS One"},{"key":"key20261025073152_b42-mmr-34-6-14042","doi-asserted-by":"publisher","first-page":"52","DOI":"10.1186\/s12964-025-02633-x","article-title":"Mechanoresponsive ion channels Piezo1 and TRPV4 stimulate ADAM10 and ADAM17 with differential impact on endothelial migration and proliferation","volume":"24","author":"Pabst","year":"2026","journal-title":"Cell Commun Signal"},{"key":"key20261025073152_b43-mmr-34-6-14042","doi-asserted-by":"publisher","first-page":"2257","DOI":"10.2147\/JIR.S448903","article-title":"Piezo1 knockout improves post-stroke cognitive dysfunction by inhibiting the interleukin-6 (IL-6)\/glutathione peroxidase 4 (GPX4) pathway","volume":"17","author":"Tang","year":"2024","journal-title":"J Inflamm Res"},{"key":"key20261025073152_b44-mmr-34-6-14042","doi-asserted-by":"publisher","first-page":"1188","DOI":"10.2174\/1871527315666160920123911","article-title":"Blood-brain barrier changes in high altitude","volume":"15","author":"Lafuente","year":"2016","journal-title":"CNS Neurol Disord Drug Targets"},{"key":"key20261025073152_b45-mmr-34-6-14042","doi-asserted-by":"publisher","first-page":"mjac036","DOI":"10.1093\/jmcb\/mjac036","article-title":"NRF1-mediated microglial activation triggers high-altitude cerebral edema","volume":"14","author":"Wang","year":"2022","journal-title":"J Mol Cell Biol"},{"key":"key20261025073152_b46-mmr-34-6-14042","doi-asserted-by":"publisher","first-page":"102026","DOI":"10.1016\/j.arr.2023.102026","article-title":"Mechanosensitive Piezo1 channel in physiology and pathophysiology of the central nervous system","volume":"90","author":"Zong","year":"2023","journal-title":"Ageing Res Rev"},{"key":"key20261025073152_b47-mmr-34-6-14042","doi-asserted-by":"publisher","first-page":"902","DOI":"10.1139\/apnm-2019-0292","article-title":"Shear stress and flow-mediated dilation in the normoxic and hypoxic human","volume":"44","author":"Tremblay","year":"2019","journal-title":"Appl Physiol Nutr Metab"}],"container-title":["Molecular Medicine Reports"],"original-title":[],"deposited":{"date-parts":[[2026,10,25]],"date-time":"2026-10-25T05:31:59Z","timestamp":1792906319000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.spandidos-publications.com\/10.3892\/mmr.2026.14042"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,13]]},"references-count":47,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2026,10,13]]}},"URL":"https:\/\/doi.org\/10.3892\/mmr.2026.14042","relation":{},"ISSN":["1791-2997","1791-3004"],"issn-type":[{"value":"1791-2997","type":"print"},{"value":"1791-3004","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,13]]}}}