{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T21:20:42Z","timestamp":1770412842192,"version":"3.49.0"},"reference-count":35,"publisher":"Spandidos Publications","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,4]]},"DOI":"10.3892\/or.2016.4570","type":"journal-article","created":{"date-parts":[[2016,1,18]],"date-time":"2016-01-18T08:47:59Z","timestamp":1453106879000},"page":"1987-1994","source":"Crossref","is-referenced-by-count":6,"title":["Constitutive expression and anticancer potency of a novel immunotoxin onconase-DV3"],"prefix":"10.3892","volume":"35","author":[{"given":"MIAONAN","family":"SUN","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"HUICHUN","family":"TANG","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"YAN","family":"GAO","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"XINXUAN","family":"DAI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"YUE","family":"YUAN","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"CHUNMEI","family":"ZHANG","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"DEJUN","family":"SUN","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2249","published-online":{"date-parts":[[2016,1,18]]},"reference":[{"key":"key20180107210935_b1-or-35-04-1987","first-page":"169","article-title":"Cytostatic and cytotoxic effects of Pannon (P-30 protein), a novel anticancer agent","volume":"21","author":"Darzynkiewicz","year":"1988","journal-title":"Cell Tissue Kinet"},{"key":"key20180107210935_b2-or-35-04-1987","doi-asserted-by":"crossref","first-page":"1141","DOI":"10.1016\/0022-2836(94)90017-5","article-title":"Refined 1.7 A X-ray crystallographic structure of P-30 protein, an amphibian ribonuclease with anti-tumor activity","volume":"236","author":"Mosimann","year":"1994","journal-title":"J Mol Biol"},{"key":"key20180107210935_b3-or-35-04-1987","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/S0021-9258(18)52427-3","article-title":"Amino acid sequence of an anti-tumor protein from Rana pipiens oocytes and early embryos. Homology to pancreatic ribonucleases","volume":"266","author":"Ardelt","year":"1991","journal-title":"J Biol Chem"},{"key":"key20180107210935_b4-or-35-04-1987","doi-asserted-by":"crossref","first-page":"5772","DOI":"10.1074\/jbc.M311233200","article-title":"Effect of N-terminal and Met23 mutations on the structure and dynamics of onconase","volume":"279","author":"Gorbatyuk","year":"2004","journal-title":"J Biol Chem"},{"key":"key20180107210935_b5-or-35-04-1987","doi-asserted-by":"crossref","first-page":"9893","DOI":"10.1074\/jbc.275.14.9893","article-title":"A ribonuclease A variant with low catalytic activity but high cytotoxicity","volume":"275","author":"Bretscher","year":"2000","journal-title":"J Biol Chem"},{"key":"key20180107210935_b6-or-35-04-1987","doi-asserted-by":"crossref","first-page":"10686","DOI":"10.1016\/S0021-9258(18)82252-9","article-title":"A cytotoxic ribonuclease. Study of the mechanism of onconase cytotoxicity","volume":"268","author":"Wu","year":"1993","journal-title":"J Biol Chem"},{"key":"key20180107210935_b7-or-35-04-1987","doi-asserted-by":"crossref","first-page":"15142","DOI":"10.1074\/jbc.M108115200","article-title":"Entry into cells and selective degradation of tRNAs by a cytotoxic member of the RNase A family","volume":"277","author":"Saxena","year":"2002","journal-title":"J Biol Chem"},{"key":"key20180107210935_b8-or-35-04-1987","doi-asserted-by":"crossref","first-page":"12201","DOI":"10.1074\/jbc.M504488200","article-title":"Transfer RNA cleavages by onconase reveal unusual cleavage sites","volume":"281","author":"Suhasini","year":"2006","journal-title":"J Biol Chem"},{"key":"key20180107210935_b9-or-35-04-1987","doi-asserted-by":"crossref","first-page":"1405","DOI":"10.1242\/jcs.03427","article-title":"Intracellular pathway of onconase that enables its delivery to the cytosol","volume":"120","author":"Rodr\u00edguez","year":"2007","journal-title":"J Cell Sci"},{"key":"key20180107210935_b10-or-35-04-1987","doi-asserted-by":"crossref","first-page":"528","DOI":"10.1182\/blood.V97.2.528","article-title":"Potent and specific antitumor effects of an anti-CD22-targeted cytotoxic ribonuclease: Potential for the treatment of non-Hodgkin lymphoma","volume":"97","author":"Newton","year":"2001","journal-title":"Blood"},{"key":"key20180107210935_b11-or-35-04-1987","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1517\/14712598.6.4.391","article-title":"Ranpirnase - an antitumour ribonuclease: Its potential role in malignant mesothelioma","volume":"6","author":"Pavlakis","year":"2006","journal-title":"Expert Opin Biol Ther"},{"key":"key20180107210935_b12-or-35-04-1987","first-page":"299","article-title":"Antitumor efficacy of the cytotoxic RNase, ranpirnase, on A549 human lung cancer xenografts of nude mice","volume":"27","author":"Lee","year":"2007","journal-title":"Anticancer Res"},{"key":"key20180107210935_b13-or-35-04-1987","doi-asserted-by":"crossref","first-page":"2276","DOI":"10.1158\/1535-7163.MCT-10-0338","article-title":"Ranpirnase (frog RNase) targeted with a humanized, internalizing, anti-Trop-2 antibody has potent cytotoxicity against diverse epithelial cancer cells","volume":"9","author":"Chang","year":"2010","journal-title":"Mol Cancer Ther"},{"key":"key20180107210935_b14-or-35-04-1987","first-page":"625","article-title":"Discovery and validation of new molecular targets for ovarian cancer","volume":"5","author":"Hu","year":"2003","journal-title":"Curr Opin Mol Ther"},{"key":"key20180107210935_b15-or-35-04-1987","doi-asserted-by":"crossref","first-page":"6570","DOI":"10.1038\/sj.onc.1206779","article-title":"Biologic and therapeutic role of HER2 in cancer","volume":"22","author":"M\u00e9nard","year":"2003","journal-title":"Oncogene"},{"key":"key20180107210935_b16-or-35-04-1987","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/j.semcancer.2003.10.003","article-title":"The significance of cancer cell expression of the chemokine receptor CXCR4","volume":"14","author":"Balkwill","year":"2004","journal-title":"Semin Cancer Biol"},{"key":"key20180107210935_b17-or-35-04-1987","doi-asserted-by":"crossref","first-page":"10646","DOI":"10.1158\/0008-5472.CAN-05-0118","article-title":"Enhanced targeting and killing of tumor cells expressing the CXC chemokine receptor 4 by transducible anticancer peptides","volume":"65","author":"Snyder","year":"2005","journal-title":"Cancer Res"},{"key":"key20180107210935_b18-or-35-04-1987","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.ab.2005.07.033","article-title":"Cell-penetrating peptides: A comparative membrane toxicity study","volume":"345","author":"Saar","year":"2005","journal-title":"Anal Biochem"},{"key":"key20180107210935_b19-or-35-04-1987","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.ab.2006.01.044","article-title":"A cell-penetrating peptide derived from mammalian cell uptake protein of Mycobacterium tuberculosis","volume":"353","author":"Lu","year":"2006","journal-title":"Anal Biochem"},{"key":"key20180107210935_b20-or-35-04-1987","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1023\/B:PHAM.0000019289.61978.f5","article-title":"Cell penetrating peptides in drug delivery","volume":"21","author":"Snyder","year":"2004","journal-title":"Pharm Res"},{"key":"key20180107210935_b21-or-35-04-1987","doi-asserted-by":"crossref","first-page":"1233","DOI":"10.1074\/jbc.M509813200","article-title":"Mechanism of uptake of C105Y, a novel cell-penetrating peptide","volume":"281","author":"Rhee","year":"2006","journal-title":"J Biol Chem"},{"key":"key20180107210935_b22-or-35-04-1987","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/j.jconrel.2009.04.028","article-title":"Cellular uptake, distribution and cytotoxicity of the hydrophobic cell penetrating peptide sequence PFVYLI linked to the proapoptotic domain peptide PAD","volume":"140","author":"Watkins","year":"2009","journal-title":"J Control Release"},{"key":"key20180107210935_b23-or-35-04-1987","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1039\/C1OB06042B","article-title":"Complexation and conjugation approaches to evaluate siRNA delivery using cationic, hydrophobic and amphiphilic peptides","volume":"10","author":"Park","year":"2012","journal-title":"Org Biomol Chem"},{"key":"key20180107210935_b24-or-35-04-1987","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1002\/yea.1208","article-title":"Heterologous protein production using the Pichia pastoris expression system","volume":"22","author":"Macauley-Patrick","year":"2005","journal-title":"Yeast"},{"key":"key20180107210935_b25-or-35-04-1987","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1007\/s00253-011-3654-z","article-title":"Protein secretion in Pichia pastoris and advances in protein production","volume":"93","author":"Damasceno","year":"2012","journal-title":"Appl Microbiol Biotechnol"},{"key":"key20180107210935_b26-or-35-04-1987","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1002\/bit.20318","article-title":"Causes of proteolytic degradation of secreted recombinant proteins produced in methylotrophic yeast Pichia pastoris: Case study with recombinant ovine interferontau","volume":"89","author":"Sinha","year":"2005","journal-title":"Biotechnol Bioeng"},{"key":"key20180107210935_b27-or-35-04-1987","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/S0168-1656(01)00254-1","article-title":"Expression of hepatitis B surface antigen in the methylotrophic yeast Pichia pastoris using the GAP promoter","volume":"88","author":"Vassileva","year":"2001","journal-title":"J Biotechnol"},{"key":"key20180107210935_b28-or-35-04-1987","doi-asserted-by":"crossref","first-page":"1355","DOI":"10.1007\/s12010-011-9524-4","article-title":"Constitutive expression of yarrowia lipolytica lipase LIP2 in Pichia pastoris using GAP as promoter","volume":"166","author":"Wang","year":"2012","journal-title":"Appl Biochem Biotechnol"},{"key":"key20180107210935_b29-or-35-04-1987","doi-asserted-by":"crossref","first-page":"588","DOI":"10.1158\/0008-5472.CAN-09-1417","article-title":"Sym004: A novel synergistic anti-epidermal growth factor receptor antibody mixture with superior anticancer efficacy","volume":"70","author":"Pedersen","year":"2010","journal-title":"Cancer Res"},{"key":"key20180107210935_b30-or-35-04-1987","doi-asserted-by":"crossref","first-page":"942","DOI":"10.1038\/sj.embor.7400491","article-title":"Control of epidermal growth factor receptor endocytosis by receptor dimerization, rather than receptor kinase activation","volume":"6","author":"Wang","year":"2005","journal-title":"EMBO Rep"},{"key":"key20180107210935_b31-or-35-04-1987","doi-asserted-by":"crossref","first-page":"27595","DOI":"10.1016\/S0021-9258(18)47026-3","article-title":"Antibody-induced epidermal growth factor receptor dimerization mediates inhibition of autocrine proliferation of A431 squamous carcinoma cells","volume":"269","author":"Fan","year":"1994","journal-title":"J Biol Chem"},{"key":"key20180107210935_b32-or-35-04-1987","doi-asserted-by":"crossref","first-page":"40183","DOI":"10.1074\/jbc.M607958200","article-title":"Epidermal growth factor receptor (EGFR) antibody down-regulates mutant receptors and inhibits tumors expressing EGFR mutations","volume":"281","author":"Perez-Torres","year":"2006","journal-title":"J Biol Chem"},{"key":"key20180107210935_b33-or-35-04-1987","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1007\/s002800050962","article-title":"Reversible nephrotoxicity of onconase and effect of lysine pH on renal onconase uptake","volume":"44","author":"Vasandani","year":"1999","journal-title":"Cancer Chemother Pharmacol"},{"key":"key20180107210935_b34-or-35-04-1987","first-page":"57","article-title":"Phase-I human clinical-trial of onconase(r) (p-30 protein) administered intravenously on a weekly schedule in cancer-patients with solid tumors","volume":"3","author":"Mikulski","year":"1993","journal-title":"Int J Oncol"},{"key":"key20180107210935_b35-or-35-04-1987","first-page":"4180","article-title":"Molecular determinants in the plasma clearance and tissue distribution of ribonucleases of the ribonuclease A superfamily","volume":"56","author":"Vasandani","year":"1996","journal-title":"Cancer Res"}],"container-title":["Oncology Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/spandidos-publications.com\/10.3892\/or.2016.4570\/download","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T21:31:04Z","timestamp":1654119064000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.spandidos-publications.com\/10.3892\/or.2016.4570"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,1,18]]},"references-count":35,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2016,1,18]]},"published-print":{"date-parts":[[2016,4]]}},"alternative-id":["10.3892\/or.2016.4570"],"URL":"https:\/\/doi.org\/10.3892\/or.2016.4570","relation":{},"ISSN":["1021-335X","1791-2431"],"issn-type":[{"value":"1021-335X","type":"print"},{"value":"1791-2431","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,1,18]]}}}