{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T11:37:18Z","timestamp":1780573038046,"version":"3.54.1"},"reference-count":36,"publisher":"Pensoft Publishers","issue":"5","license":[{"start":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T00:00:00Z","timestamp":1761091200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["FM"],"abstract":"<jats:p>\n                    \ufeff\n                    <jats:bold>Abstract<\/jats:bold>\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Introduction<\/jats:bold>\n                    : Trimethylamine-N-oxide (TMAO) is an organic compound formed when flavin monooxygenases react with trimethylamine (TMA), which can be obtained from food or produced by the intestinal microbiota. Blood levels of TMAO are positively correlated with low-grade inflammation and various health conditions, including impaired renal function, colorectal cancer, and cardiovascular disease.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Aim<\/jats:bold>\n                    : This study presents the development and validation of an LC-MS\/MS method for determining TMAO in human plasma. In the second stage, a group of 62 adults with newly diagnosed Hashimoto\u2019s thyroiditis (HT) was tested for TMAO, thyroid hormones, autoantibodies, and markers of lipid and glucose metabolism.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results<\/jats:bold>\n                    : A modified sample preparation procedure ensures that the sample is cleaned of interfering substances and proteins. Linearity was confirmed over a range of 50 \u00b5g\/L\u20133000 \u00b5g\/L, with an R\u00b2 greater than 0.995. The normalized matrix values ranged from 87.1% to 111.9%, the accuracy was between 84.0% and 112.7%, and the within-run and between-run precision were both less than 9%. The patient group had a higher average TMAO level than the controls (172.5 \u00b5g\/L vs. 158.5 \u00b5g\/L). TMAO levels were positively correlated with BMI (\n                    <jats:italic>r<\/jats:italic>\n                    =0.380,\n                    <jats:italic>p<\/jats:italic>\n                    =0.002) and anti-TPO antibodies (\n                    <jats:italic>r<\/jats:italic>\n                    =0.264,\n                    <jats:italic>p<\/jats:italic>\n                    =0.04).\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusion<\/jats:bold>\n                    : The determined TMAO levels in p atients with Hashimoto\u2019s thyroiditis were higher than in the control group, but the differences were not significant. They only indicated a trend, which may be associated with the presence of anti-TPO antibodies, the activity of the autoimmune process, and the presence of systemic inflammation.\n                  <\/jats:p>","DOI":"10.3897\/folmed.67.e152209","type":"journal-article","created":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T14:30:08Z","timestamp":1761143408000},"source":"Crossref","is-referenced-by-count":0,"title":["Plasma trimethylamine-N-oxide quantification in patients with Hashimoto\u2019s thyroiditis by using LC-MS\/MS"],"prefix":"10.3897","volume":"67","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2028-9855","authenticated-orcid":true,"given":"Desislav G.","family":"Tomov","sequence":"first","affiliation":[{"name":"Research Institute at the Medical University of Plovdiv, Plovdiv, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0215-6119","authenticated-orcid":true,"given":"Boryana","family":"Levterova","sequence":"additional","affiliation":[{"name":"Medical University, Plovdiv, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3845-2072","authenticated-orcid":true,"given":"Valentina","family":"Mihaylova","sequence":"additional","affiliation":[{"name":"Medical University, Plovdiv, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9066-4537","authenticated-orcid":true,"given":"Dimitar","family":"Troev","sequence":"additional","affiliation":[{"name":"Medical University, Plovdiv, Bulgaria"},{"name":"St George University Hospital, Plovdiv, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1607-2829","authenticated-orcid":true,"given":"Zlatina","family":"Tomova","sequence":"additional","affiliation":[{"name":"Medical University, Plovdiv, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6423-5998","authenticated-orcid":true,"given":"Yordanka","family":"Uzunova","sequence":"additional","affiliation":[{"name":"Medical University, Plovdiv, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9918-0707","authenticated-orcid":true,"given":"Maria","family":"Orbetzova","sequence":"additional","affiliation":[{"name":"Medical University, Plovdiv, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2258","published-online":{"date-parts":[[2025,10,22]]},"reference":[{"key":"152209_B1","doi-asserted-by":"crossref","unstructured":"1. 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Plasma concentrations of trimethylamine-N-oxide are directly associated with dairy food consumption and low-grade inflammation in a german adult population. J Nutr 2016; 146(2):283\u20139.","DOI":"10.3945\/jn.115.220103"},{"key":"152209_B18","doi-asserted-by":"crossref","unstructured":"18. Kr\u00fcger R, Merz B, Rist MJ, et al. Associations of current diet with plasma and urine TMAO in the KarMeN study: direct and indirect contributions. Mol Nutr Food Res 2017; 61(11):1\u201326.","DOI":"10.1002\/mnfr.201700363"},{"key":"152209_B19","doi-asserted-by":"crossref","unstructured":"19. Rath S, Rox K, Kleine Bardenhorst S, et al. Higher trimethylamine-N-oxide plasma levels with increasing age are mediated by diet and trimethylamine-forming bacteria. mSystems 2021; 6(5):10\u2013128.","DOI":"10.1128\/msystems.00945-21"},{"key":"152209_B20","doi-asserted-by":"crossref","unstructured":"20. Virili C, Fallahi P, Antonelli A, et al. Gut microbiota and Hashimoto\u2019s thyroiditis. 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