{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T15:05:23Z","timestamp":1791644723769,"version":"4.3.4"},"reference-count":43,"publisher":"Pensoft Publishers","issue":"2","license":[{"start":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T00:00:00Z","timestamp":1776643200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["FM"],"abstract":"<jats:p>\n                    <jats:bold>Aim<\/jats:bold>\n                    : Functional mandibular advancement appliances are widely used in orthodontics, but their molecular and histological effects on masticatory muscles remain underexplored. This study evaluated gene expression and tissue-level adaptations in response to mandibular advancement and a subsequent recovery period in young male Wistar rats.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Materials and methods<\/jats:bold>\n                    : Thirty rats were randomly assigned to three groups: control (Group A), functional mandibular advancement for four weeks (Group B), and functional mandibular advancement followed by a one-month recovery period (Group C). The expression of myostatin and myosin-1c genes was determined using quantitative real-time reverse transcription polymerase chain reaction. Histological analyses were performed to examine muscle fiber morphology in the masseter and lateral pterygoid muscles.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results<\/jats:bold>\n                    : Mandibular advancement produced a significant downregulation of myostatin and upregulation of myosin-1c (\n                    <jats:italic>p<\/jats:italic>\n                    &amp;lt;0.05), most prominent in the lateral pterygoid muscle. Histological evaluation revealed hypertrophic changes in muscle fibers consistent with increased mechanical loading. After the recovery phase, altered gene expression and hypertrophic features persisted partially, indicating sustained molecular and structural adaptations. The masseter and lateral pterygoid muscles exhibited distinct, muscle-specific responses at both the genetic and histological levels.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusion<\/jats:bold>\n                    : Mandibular advancement induces molecular and histological adaptations in masticatory muscles, with partial persistence following a one-month recovery period. These results suggest the potential for long-term functional and morphological changes after orthodontic intervention and provide biological evidence to inform personalized treatment and retention strategies in growing individuals.\n                  <\/jats:p>","DOI":"10.3897\/folmed.68.e169836","type":"journal-article","created":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T13:27:22Z","timestamp":1776691642000},"source":"Crossref","is-referenced-by-count":1,"title":["Unveiling molecular dynamics: the effects of functional mandibular advancement and a one-month recovery period on myostatin and myosin-1c gene expression in masticatory muscles of young male Wistar rats"],"prefix":"10.3897","volume":"68","author":[{"given":"Sonakshi","family":"Sharma","sequence":"first","affiliation":[{"name":"Bharati Vidyapeeth Dental college and hospital, Pune, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8636-4336","authenticated-orcid":true,"given":"Amol","family":"Patil","sequence":"additional","affiliation":[{"name":"Bharati Vidyapeeth Dental college and hospital, Pune, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shruti","family":"Biyani","sequence":"additional","affiliation":[{"name":"Bharati Vidyapeeth Dental college and hospital, Pune, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shivangini","family":"Swarnkar","sequence":"additional","affiliation":[{"name":"Bharati Vidyapeeth Dental college and hospital, Pune, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6564-4227","authenticated-orcid":true,"given":"Meydha","family":"Gera","sequence":"additional","affiliation":[{"name":"Bharati Vidyapeeth Dental college and hospital, Pune, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2258","published-online":{"date-parts":[[2026,4,20]]},"reference":[{"key":"169836_B1"},{"key":"169836_B2"},{"key":"169836_B3"},{"key":"169836_B4"},{"key":"169836_B5"},{"key":"169836_B6"},{"key":"169836_B7"},{"key":"169836_B8","unstructured":"Proffit WR, Fields HW, Larson B, et al. Contemporary Orthodontics. 6th South Asia ed. New Delhi: Elsevier India; 2019."},{"key":"169836_B9"},{"key":"169836_B10"},{"key":"169836_B11"},{"key":"169836_B12"},{"key":"169836_B13"},{"key":"169836_B14"},{"key":"169836_B15"},{"key":"169836_B16"},{"key":"169836_B17"},{"key":"169836_B18"},{"key":"169836_B19"},{"key":"169836_B20"},{"key":"169836_B21"},{"key":"169836_B22"},{"key":"169836_B23"},{"key":"169836_B24"},{"key":"169836_B25"},{"key":"169836_B26"},{"key":"169836_B27"},{"key":"169836_B28"},{"key":"169836_B29"},{"key":"169836_B30"},{"key":"169836_B31"},{"key":"169836_B32"},{"key":"169836_B33"},{"key":"169836_B34"},{"key":"169836_B35"},{"key":"169836_B36"},{"key":"169836_B37"},{"key":"169836_B38"},{"key":"169836_B39"},{"key":"169836_B40"},{"key":"169836_B41"},{"key":"169836_B42"},{"key":"169836_B43"}],"container-title":["Folia Medica"],"original-title":[],"link":[{"URL":"https:\/\/foliamedica.bg\/article\/169836\/download\/pdf\/","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/foliamedica.bg\/article\/169836\/download\/xml\/","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/foliamedica.bg\/article_preview.php?id=169836&skip_redirect=1","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T13:27:23Z","timestamp":1776691643000},"score":1,"resource":{"primary":{"URL":"https:\/\/foliamedica.bg\/article\/169836\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,20]]},"references-count":43,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2026,4,20]]}},"URL":"https:\/\/doi.org\/10.3897\/folmed.68.e169836","relation":{},"ISSN":["1314-2143","0204-8043"],"issn-type":[{"value":"1314-2143","type":"electronic"},{"value":"0204-8043","type":"print"}],"subject":[],"published":{"date-parts":[[2026,4,20]]},"article-number":"e169836"}}