{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T17:59:53Z","timestamp":1792519193568,"version":"4.5.0"},"reference-count":104,"publisher":"Pensoft Publishers","license":[{"start":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T00:00:00Z","timestamp":1771200000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["imafungus"],"abstract":"<jats:p>\n                    Ambrosia beetles (\n                    <jats:italic>Coleoptera<\/jats:italic>\n                    ,\n                    <jats:italic>Curculionidae<\/jats:italic>\n                    ) form obligate nutritional symbioses with ambrosia fungi cultivated within their galleries. Among them, the pinhole borers (\n                    <jats:italic>Platypodinae<\/jats:italic>\n                    ) are predominantly tropical, with only two representatives native to Europe. One of them, the rare and understudied\n                    <jats:italic>Treptoplatypus oxyurus<\/jats:italic>\n                    , primarily colonises\n                    <jats:italic>Abies alba<\/jats:italic>\n                    . We investigated its fungal symbionts using a cultivation-dependent approach. We identified three numerically dominant associates in the prothorax containing mycangia:\n                    <jats:italic>Candida schatavii<\/jats:italic>\n                    ,\n                    <jats:italic>Magnusiomyces fungicola<\/jats:italic>\n                    , and a novel member of\n                    <jats:italic>Ophiostomatales<\/jats:italic>\n                    . The latter,\n                    <jats:italic>Wilhelmdebeerea oxyuri<\/jats:italic>\n                    <jats:bold>gen. et sp. nov<\/jats:bold>\n                    ., was the most abundant and exhibited both leptographium-like and hyalorhinocladiella-like morphs. Additionally, two new yeast species of low abundance and uncertain ecological roles were isolated and described:\n                    <jats:italic>Blastobotrys sasensis<\/jats:italic>\n                    <jats:bold>sp. nov<\/jats:bold>\n                    . and\n                    <jats:italic>Sugiyamaella casensis<\/jats:italic>\n                    <jats:bold>sp. nov<\/jats:bold>\n                    ., both belonging to the family\n                    <jats:italic>Trichomonascaceae (Dipodascales)<\/jats:italic>\n                    . Multigene and phylogenomics analyses confirmed the distinct taxonomic placement of all three new species. The ecological roles of the identified fungi and the strength of their association with\n                    <jats:italic>T. oxyurus<\/jats:italic>\n                    require confirmation through further studies at additional locations. Our findings reveal a previously undocumented fungal diversity tightly linked to a unique pinhole borer,\n                    <jats:italic>T. oxyurus<\/jats:italic>\n                    , thereby enriching our understanding of the fungi associated with conifer-colonising beetles and their ecological and biotechnological importance.\n                  <\/jats:p>","DOI":"10.3897\/imafungus.17.177075","type":"journal-article","created":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T13:43:14Z","timestamp":1771249394000},"source":"Crossref","is-referenced-by-count":2,"title":["The ambrosial mycobiota of Treptoplatypus oxyurus (Coleoptera, Platypodidae): a unique island of fungal diversity revealing Wilhelmdebeerea oxyuri gen. et sp. nov. (Ophiostomatales), and two new yeast species Blastobotrys sasensis sp. nov., and Sugiyamaella casensis sp. nov. (Dipodascales)"],"prefix":"10.3897","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4016-0335","authenticated-orcid":true,"given":"Miroslav","family":"Kola\u0159\u00edk","sequence":"first","affiliation":[{"name":"Institute of Microbiology of the Czech Academy of Sciences, Praha, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1401-5604","authenticated-orcid":true,"given":"Renata","family":"Vadkertiov\u00e1","sequence":"additional","affiliation":[{"name":"Culture Collection of Yeasts, Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3221-9448","authenticated-orcid":true,"given":"Milo\u0161","family":"Kn\u00ed\u017eek","sequence":"additional","affiliation":[{"name":"Department of Forest Protection Service, Forestry and Game Management Research Institute, J\u00edlovi\u0161t\u011b, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8714-8558","authenticated-orcid":true,"given":"Franti\u0161ek","family":"Sklen\u00e1\u0159","sequence":"additional","affiliation":[{"name":"Institute of Microbiology of the Czech Academy of Sciences, Praha, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8662-1817","authenticated-orcid":true,"given":"Jozef","family":"Vakula","sequence":"additional","affiliation":[{"name":"Science and Research Division, National Forest Centre, Bansk\u00e1 \u0160tiavnica, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3627-5121","authenticated-orcid":true,"given":"Milan","family":"Z\u00fabrik","sequence":"additional","affiliation":[{"name":"Science and Research Division, National Forest Centre, Bansk\u00e1 \u0160tiavnica, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4593-1525","authenticated-orcid":true,"given":"Michal","family":"Kol\u00e1\u0159","sequence":"additional","affiliation":[{"name":"Institute of Molecular Genetics of the Czech Academy of Sciences, Praha, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8706-4618","authenticated-orcid":true,"given":"Ji\u0159\u00ed","family":"Hulcr","sequence":"additional","affiliation":[{"name":"Institute of Microbiology of the Czech Academy of Sciences, Praha, Czech Republic"},{"name":"School of Forest, Fisheries and Geomatics Sciences, University of Florida, Gainesville, United States of America"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2258","published-online":{"date-parts":[[2026,2,16]]},"reference":[{"key":"177075_B1","doi-asserted-by":"publisher","DOI":"10.1093\/forestry\/cpm029"},{"key":"177075_B2","unstructured":"Andrews S (2010) FastQC: a quality control tool for high throughput sequence data. http:\/\/www.bioinformatics.babraham.ac.uk\/projects\/fastqc\/"},{"key":"177075_B3","doi-asserted-by":"publisher","DOI":"10.48162\/rev.39.145"},{"key":"177075_B4","doi-asserted-by":"publisher","DOI":"10.1093\/g3journal\/jkab342"},{"key":"177075_B5","doi-asserted-by":"publisher","DOI":"10.1016\/j.funeco.2016.07.001"},{"key":"177075_B6","doi-asserted-by":"publisher","DOI":"10.1089\/cmb.2012.0021"},{"key":"177075_B7","doi-asserted-by":"publisher","DOI":"10.1007\/BF03053133"},{"key":"177075_B8","doi-asserted-by":"publisher","DOI":"10.5897\/AJMR11.614"},{"key":"177075_B9","doi-asserted-by":"publisher","DOI":"10.1111\/2041-210x.12073"},{"key":"177075_B10","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2015.10.006"},{"key":"177075_B11","unstructured":"B\u00f6hme J (1994) Bemerkenswerte K\u00e4ferfunde aus Mitteleuropa. 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