{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T14:33:02Z","timestamp":1792679582053,"version":"4.5.0"},"reference-count":65,"publisher":"Pensoft Publishers","license":[{"start":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T00:00:00Z","timestamp":1787702400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["imafungus"],"abstract":"<jats:p>\n                    The phylogenetic affiliations of anaerobic gut fungi (\n                    <jats:italic>Neocallimastigomycota<\/jats:italic>\n                    ) are typically evaluated using single-gene markers. However, this approach often fails to resolve relationships between closely related lineages. To address this issue and identify alternative markers, we created a curated database comprising the complete ribosomal operon sequences of 156 isolates, representing 20 of the 22 recognized genera and two new genus-level clades. Using long-read sequencing, we obtained ~9 kbp operon sequences and developed a robust analysis pipeline. Incorporating both coding genes and non-coding regions (excluding IGS1) improved phylogenetic resolution. This phylogenetic approach successfully resolved the\n                    <jats:italic>Cyllamyces<\/jats:italic>\n                    and\n                    <jats:italic>Caecomyces<\/jats:italic>\n                    clades (hard-to-distinguish genetically), as well as seven analysed\n                    <jats:italic>Piromyces<\/jats:italic>\n                    species. We also scanned the operon for markers that are suitable for short-read sequencing platforms, with the aim of enhancing biodiversity and phylogenetic studies. Notably, the ETS1 genetic region also enabled the distinction between these lineages, indicating its phylogenetic value within the ribosomal operon. The resulting database is a valuable resource for expanding and strengthening phylogenetic frameworks.\n                  <\/jats:p>","DOI":"10.3897\/imafungus.17.195921","type":"journal-article","created":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T13:12:42Z","timestamp":1787749962000},"source":"Crossref","is-referenced-by-count":1,"title":["Full ribosomal operon sequencing of anaerobic gut fungi (phylum Neocallimastigomycota): insights on its markers and phylogenetic resolution"],"prefix":"10.3897","volume":"17","author":[{"given":"Diana","family":"Young","sequence":"first","affiliation":[{"name":"Department for Laboratory Analytics, Micro and Molecular Biology, Bavarian State Research Center for Agriculture, Freising, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katrin","family":"St\u00fcer-Patowsky","sequence":"additional","affiliation":[{"name":"Chair of Urban Water Systems Engineering, Technical University of Munich, Garching, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liren","family":"Huang","sequence":"additional","affiliation":[{"name":"Faculty of Technology and Center for Biotechnology (CeBiTec), Bielefeld University, Bielefeld, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mostafa S.","family":"Elshahed","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, Oklahoma State University, Stillwater, United States of America"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noha H.","family":"Youssef","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, Oklahoma State University, Stillwater, United States of America"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Radwa","family":"Hanafy","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, Oklahoma State University, Stillwater, United States of America"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanfen","family":"Cheng","sequence":"additional","affiliation":[{"name":"Laboratory of Gastrointestinal Microbiology, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina D.","family":"Moon","sequence":"additional","affiliation":[{"name":"AgResearch Group, Bioeconomy Science Institute, Palmerston North, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Priya","family":"Soni","sequence":"additional","affiliation":[{"name":"AgResearch Group, Bioeconomy Science Institute, Palmerston North, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akshay","family":"Joshi","sequence":"additional","affiliation":[{"name":"Center of Environmental Biotechnology, Institute for Chemistry and Biotechnology, Z\u00fcrich University of Applied Sciences, W\u00e4denswil, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcus","family":"Stabel","sequence":"additional","affiliation":[{"name":"Process Engineering in Life Sciences 2: Technical Biology, Karlsruhe Institute of Technology, Karlsruhe, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katrin","family":"Ochsenreither","sequence":"additional","affiliation":[{"name":"Applied Logistics and Polymer Sciences: Pharmaceutical Biotechnology, University of Applied Sciences Kaiserslautern, Pirmasens, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sumit Singh","family":"Dagar","sequence":"additional","affiliation":[{"name":"Bioenergy Group, Agharkar Research Institute, Pune, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ethan","family":"Hillman","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, University of Michigan, Ann Arbor, United States of America"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin V.","family":"Solomon","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering, University of Delaware, Newark, United States of America"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katerina O.","family":"Fliegerov\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Animal Physiology and Genetics, Czech Academy of Sciences, Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gareth W.","family":"Griffith","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, Aberystwyth University, Aberystwyth, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tony M.","family":"Callaghan","sequence":"additional","affiliation":[{"name":"SomaTech Ltd. 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