{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T16:12:55Z","timestamp":1789143175944,"version":"build-2803163510"},"reference-count":53,"publisher":"Pensoft Publishers","license":[{"start":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T00:00:00Z","timestamp":1789084800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["imafungus"],"abstract":"<jats:p>\n                    Uncovering the identity of \u201cdark fungi\u201d is crucial for understanding fungal taxonomy, diversity, and phylogenetic relationships. Previous exploratory analysis of the mycorrhizal fungi of the partially mycoheterotrophic orchid\n                    <jats:italic>Nervilia nipponica<\/jats:italic>\n                    revealed an association with a particular group of\n                    <jats:italic>Agaricomycetes<\/jats:italic>\n                    , tentatively designated \u201cunidentified\n                    <jats:italic>Agaricomycetes<\/jats:italic>\n                    X\u201d (uAX). Two culture strains were successfully isolated from the mycorrhiza, which enabled the phylogenetic position of this fungus to be determined using ultrastructural and multigene phylogenetic analyses. Transmission electron microscopy observations of the two cultures of uAX revealed that the dolipore septum is associated with perforate septal pore caps (SPCs), the margins of which are reflexed outwards. This combination of characters has not previously been reported in any order of\n                    <jats:italic>Basidiomycota<\/jats:italic>\n                    . Combined phylogenetic analyses based on sequence data from four loci [5.8S and 28S ribosomal DNA (rDNA), translation elongation factor 1-\u03b1, and RNA polymerase II second large subunit] indicate that uAX is an early-diverging clade of\n                    <jats:italic>Agaricomycetes<\/jats:italic>\n                    that cannot be accommodated in any known order of\n                    <jats:italic>Basidiomycota<\/jats:italic>\n                    . The name\n                    <jats:italic>Reflexicalyptrales<\/jats:italic>\n                    is proposed here for this new order of\n                    <jats:italic>Basidiomycota<\/jats:italic>\n                    . Furthermore, BLAST searches of the internal transcribed spacers of nuclear ribosomal DNA (nrDNA) and phylogenetic analyses reveal that closely related taxa have previously been detected in soil, leaf litter, and living root samples collected at various locations worldwide. This suggests that the group is ubiquitously distributed and probably exhibits a saprotrophic lifestyle\n                  <\/jats:p>","DOI":"10.3897\/imafungus.17.201939","type":"journal-article","created":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T15:45:22Z","timestamp":1789141522000},"source":"Crossref","is-referenced-by-count":0,"title":["Reflexicalyptrales ordo nov., a distinct Agaricomycetes lineage discovered from orchid mycorrhizae"],"prefix":"10.3897","volume":"17","author":[{"given":"Akihiko","family":"Kinoshita","sequence":"first","affiliation":[{"name":"Kyushu Research Center, Forestry and Forest Products Research Institute, Kumamoto, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shohei","family":"Fujimori","sequence":"additional","affiliation":[{"name":"Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kensuke","family":"Seto","sequence":"additional","affiliation":[{"name":"Sugadaira Research Station, Mountain Science Center, University of Tsukuba, Nagano, Japan"},{"name":"Institute for Multidisciplinary Sciences (IMS), Yokohama National University, Yokohama, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuki","family":"Ogura-Tsujita","sequence":"additional","affiliation":[{"name":"Faculty of Agriculture, Saga University, Saga, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayako","family":"Maeda","sequence":"additional","affiliation":[{"name":"The Kochi Prefectural Makino Botanical Garden, Kochi, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephan W.","family":"Gale","sequence":"additional","affiliation":[{"name":"Kadoorie Farm and Botanic Garden, Hong Kong S.A.R., China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junichi P.","family":"Abe","sequence":"additional","affiliation":[{"name":"Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yousuke","family":"Degawa","sequence":"additional","affiliation":[{"name":"Institute for Multidisciplinary Sciences (IMS), Yokohama National University, Yokohama, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomohisa","family":"Yukawa","sequence":"additional","affiliation":[{"name":"Tsukuba Botanical Garden, National Museum of Nature and Science, Tsukuba, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2258","published-online":{"date-parts":[[2026,9,11]]},"reference":[{"key":"201939_B1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkad1039"},{"key":"201939_B2","doi-asserted-by":"publisher","DOI":"10.1038\/ng0294-119"},{"key":"201939_B3","doi-asserted-by":"publisher","DOI":"10.1007\/s13225-021-00472-y"},{"key":"201939_B4","doi-asserted-by":"publisher","DOI":"10.1007\/s11557-016-1220-x"},{"key":"201939_B5","doi-asserted-by":"publisher","DOI":"10.3852\/09-288"},{"key":"201939_B6","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.molbev.a026334"},{"key":"201939_B7","unstructured":"Connolly JH, Arnott HJ, Jellison J (1996) Patterns of calcium oxalate crystal production by three species of wood decay fungi. 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