{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T15:14:25Z","timestamp":1783437265201,"version":"3.54.6"},"reference-count":44,"publisher":"Pensoft Publishers","license":[{"start":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T00:00:00Z","timestamp":1783382400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100005156","name":"Alexander von Humboldt-Stiftung","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005156","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010665","name":"H2020 Marie Sk\u0142odowska-Curie Actions","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MC"],"abstract":"<jats:p>\n                    A decahydrofluorene-class alkaloid, pyrrospirone Z (\n                    <jats:bold>1a<\/jats:bold>\n                    ), along with pyrrospirone F (\n                    <jats:bold>2<\/jats:bold>\n                    ), pyrrospirone M (\n                    <jats:bold>3<\/jats:bold>\n                    ), GKK1032A2 (\n                    <jats:bold>4<\/jats:bold>\n                    ), daldinol (\n                    <jats:bold>5<\/jats:bold>\n                    ), orthosporin (\n                    <jats:bold>6<\/jats:bold>\n                    ), and 3,4-dihydro-3,4,8-trihydroxy-1(2H)-naphthalenone (\n                    <jats:bold>7<\/jats:bold>\n                    ), was purified from the new coprophilous species\n                    <jats:italic>Schizothecium keniense<\/jats:italic>\n                    , isolated from dung in Kenya. Structural elucidation was achieved using comprehensive 1D and 2D NMR spectroscopy, and high-resolution electrospray ionization mass spectrometry (HR-ESI-MS), with relative configurations assigned based on NOESY correlations. Among the compounds, pyrrospirone F (\n                    <jats:bold>2<\/jats:bold>\n                    ) and GKK1032A2 (\n                    <jats:bold>4<\/jats:bold>\n                    ) displayed significant antibacterial activity against the Gram-positive bacteria\n                    <jats:italic>Bacillus subtilis<\/jats:italic>\n                    (MIC = 4.2 \u03bcg\/mL and 8.3 \u03bcg\/mL, respectively) and\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    (MIC = 8.3 \u03bcg\/mL for both). Additionally, compounds\n                    <jats:bold>2<\/jats:bold>\n                    and\n                    <jats:bold>4<\/jats:bold>\n                    showed mild antifungal activity against the filamentous fungi\n                    <jats:italic>Mucor hiemalis<\/jats:italic>\n                    at 33.3 \u03bcg\/mL. Cytotoxicity assays revealed that GKK1032A2 (\n                    <jats:bold>4<\/jats:bold>\n                    ) was the most potent compound, with IC\n                    <jats:sub>50<\/jats:sub>\n                    values ranging from 2.0 \u00b5M to 8.1 \u00b5M across the tested human cancer cell lines. This work represents the first investigation of the chemical constituents of the genus\n                    <jats:italic>Schizothecium<\/jats:italic>\n                    and underscores the potential of coprophilous fungi as valuable sources of biologically active metabolites.\n                  <\/jats:p>","DOI":"10.3897\/mycokeys.136.191424","type":"journal-article","created":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T14:21:07Z","timestamp":1783434067000},"page":"139-159","source":"Crossref","is-referenced-by-count":0,"title":["Pyrrospirone Z and related decahydrofluorene alkaloids produced by the new coprophilous species Schizothecium keniense (Schizotheciaceae, Sordariales)"],"prefix":"10.3897","volume":"136","author":[{"given":"Manuela","family":"Agudelo-Restrepo","sequence":"first","affiliation":[{"name":"Department of Microbial Drugs, Helmholtz Centre for Infection Research GmbH (HZI), and German Centre for Infection Research Association (DZIF), Braunschweig, Germany"},{"name":"Institute of Microbiology, Technische Universit\u00e4t Braunschweig, Braunschweig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Dibouloul","sequence":"additional","affiliation":[{"name":"Department of Microbial Drugs, Helmholtz Centre for Infection Research GmbH (HZI), and German Centre for Infection Research Association (DZIF), Braunschweig, Germany"},{"name":"Department of Chemistry, Higher Teacher Training College, University of Yaounde 1, Yaounde, Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen","family":"Harms","sequence":"additional","affiliation":[{"name":"Department of Microbial Drugs, Helmholtz Centre for Infection Research GmbH (HZI), and German Centre for Infection Research Association (DZIF), Braunschweig, Germany"},{"name":"Institute of Microbiology, Technische Universit\u00e4t Braunschweig, Braunschweig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph","family":"Tchamgoue","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Higher Teacher Training College, University of Yaounde 1, Yaounde, Cameroon"},{"name":"Department of Organic Chemistry, Faculty of Science, University of Yaounde 1, Yaounde, Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc","family":"Stadler","sequence":"additional","affiliation":[{"name":"Department of Microbial Drugs, Helmholtz Centre for Infection Research GmbH (HZI), and German Centre for Infection Research Association (DZIF), Braunschweig, Germany"},{"name":"Institute of Microbiology, Technische Universit\u00e4t Braunschweig, Braunschweig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josphat","family":"Matasyoh","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Egerton University, Egerton, Kenya"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simeon Fogue","family":"Kouam","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Higher Teacher Training College, University of Yaounde 1, Yaounde, Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasmina","family":"Marin-Felix","sequence":"additional","affiliation":[{"name":"Department of Microbial Drugs, Helmholtz Centre for Infection Research GmbH (HZI), and German Centre for Infection Research Association (DZIF), Braunschweig, Germany"},{"name":"Institute of Microbiology, Technische Universit\u00e4t Braunschweig, Braunschweig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2258","published-online":{"date-parts":[[2026,7,7]]},"reference":[{"key":"191424_B1","doi-asserted-by":"publisher","DOI":"10.3897\/imafungus.17.177411"},{"key":"191424_B2","doi-asserted-by":"publisher","DOI":"10.1039\/C9RA10685E"},{"key":"191424_B3","doi-asserted-by":"publisher","DOI":"10.1021\/np50061a015"},{"key":"191424_B4","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jnatprod.5c01203"},{"key":"191424_B5","doi-asserted-by":"publisher","DOI":"10.25664\/art-0180"},{"key":"191424_B6","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2013.08.001"},{"key":"191424_B7","unstructured":"Cai L, Jeewon R, Hyde KD (2005) Phylogenetic evaluation and taxonomic revision of Schizothecium based on ribosomal DNA and protein-coding genes. 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