{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T09:27:01Z","timestamp":1786699621380,"version":"build-2736575974"},"reference-count":44,"publisher":"Pensoft Publishers","license":[{"start":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T00:00:00Z","timestamp":1786665600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ZK"],"abstract":"<jats:p>\n                    Two new sympatric cavernicolous species of the millipede genus\n                    <jats:italic>Plusioglyphiulus<\/jats:italic>\n                    Silvestri, 1923, are described from Battambang Province, northwestern Cambodia:\n                    <jats:italic>Plusioglyphiulus parviserratus<\/jats:italic>\n                    Likhitrakarn &amp; Seesamut,\n                    <jats:bold>sp. nov<\/jats:bold>\n                    . and\n                    <jats:italic>Plusioglyphiulus battambangensis<\/jats:italic>\n                    Likhitrakarn &amp; Seesamut,\n                    <jats:bold>sp. nov<\/jats:bold>\n                    . Both new taxa are diagnosed based on an integrated taxonomic approach combining morphological features and mitochondrial cytochrome\n                    <jats:italic>c<\/jats:italic>\n                    oxidase subunit I (COI) gene sequences from a previous study.\n                    <jats:italic>Plusioglyphiulus parviserratus<\/jats:italic>\n                    <jats:bold>sp. nov<\/jats:bold>\n                    . is distinguished from all congeners by having the male leg 1 telopodite distinctly 2-segmented with a rudimentary, knob-like second telopoditomere, the anterior gonopod coxosternal processes long, slender, and regularly curved anteriorly, resembling a bird\u2019s head, and a unique micro-serrate upper margin of the broad flagellum process. In contrast,\n                    <jats:italic>P. battambangensis<\/jats:italic>\n                    <jats:bold>sp. nov<\/jats:bold>\n                    . is characterized by a distinct carinotaxic formula of the collum, a significantly smaller second telopoditomere on the male leg 1, a slender, elongated, paramedian coxal process of the posterior gonopod, and a narrow, smooth-margined flagellum process. The two new species are separated from each other by an interspecific COI p-distance of 10.3% and show divergences of 9.6\u20139.9% from their closest known relatives, all lying well within the stable range observed for species boundaries in the Cambalopsidae and therefore strongly supporting their independent specific status.\n                  <\/jats:p>\n                  <jats:p>Beyond traditional descriptions, this study evaluates the resolution efficiency and practical limitations of multiple imaging modalities, including the standard transmission light photography, traditional line drawings, scanning electron microscopy, and micro-computed tomography scanning. While 3D\u00b5CT reconstructions provide powerful, non-destructive visualizations of external somatic architectures, we demonstrate that current in-situ tomographic data remain insufficient for resolving the highly condensed, tightly appressed gonopodial microscopic characters, thereby emphasizing the continued necessity of physical dissection and SEM in cambalopsid taxonomy.<\/jats:p>\n                  <jats:p>\n                    These findings increase the number of\n                    <jats:italic>Plusioglyphiulus<\/jats:italic>\n                    species currently known to occur in Cambodia to six, highlighting the significant, yet severely understudied, cavernicolous diplopod diversity in the country\u2019s extensive karst systems. An updated identification key to all six Cambodian\n                    <jats:italic>Plusioglyphiulus<\/jats:italic>\n                    species is provided. These discoveries underscore the critical need for continued taxonomic exploration and the implementation of conservation strategies for the imperiled limestone karst ecosystems in the region.\n                  <\/jats:p>","DOI":"10.3897\/zookeys.1289.204580","type":"journal-article","created":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T08:28:15Z","timestamp":1786696095000},"page":"237-269","source":"Crossref","is-referenced-by-count":0,"title":["Integrated taxonomy of two new species of the millipede genus Plusioglyphiulus Silvestri, 1923 from Cambodia (Diplopoda, Spirostreptida)"],"prefix":"10.3897","volume":"1289","author":[{"given":"Natdanai","family":"Likhitrakarn","sequence":"first","affiliation":[{"name":"Division of Plant Protection, Faculty of Agricultural Production, Maejo University, Chiang Mai, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergei I.","family":"Golovatch","sequence":"additional","affiliation":[{"name":"A. N. Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruttapon","family":"Srisonchai","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Science, Khon Kaen University, Khon Kaen, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sothearen","family":"Thi","sequence":"additional","affiliation":[{"name":"Fauna & Flora Cambodia Programme, Phnom Penh, Cambodia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophea","family":"Chhin","sequence":"additional","affiliation":[{"name":"Department of Biodiversity, General Directorate of Policy and Strategy, Ministry of Environment, Phnom Penh, Cambodia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanny","family":"Lou","sequence":"additional","affiliation":[{"name":"Fauna & Flora Cambodia Programme, Phnom Penh, Cambodia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pablo","family":"Sinovas","sequence":"additional","affiliation":[{"name":"Fauna & Flora Cambodia Programme, Phnom Penh, Cambodia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Parin","family":"Jirapatrasilp","sequence":"additional","affiliation":[{"name":"Animal Systematics Research Unit, Department of Biology, Faculty of Science, Chulalongkorn University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chirasak","family":"Sutcharit","sequence":"additional","affiliation":[{"name":"Animal Systematics Research Unit, Department of Biology, Faculty of Science, Chulalongkorn University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Somsak","family":"Panha","sequence":"additional","affiliation":[{"name":"Animal Systematics Research Unit, Department of Biology, Faculty of Science, Chulalongkorn University, Bangkok, Thailand"},{"name":"Academy of Science, The Royal Society of Thailand, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teerapong","family":"Seesamut","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Science, Rangsit University, Pathumthani, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2258","published-online":{"date-parts":[[2026,8,14]]},"reference":[{"key":"204580_B1","unstructured":"Attems C (1938) Die von Dr. C. Dawydoff in Franz\u00f6sisch Indochina gesammelten Myriopoden. M\u00e9moires du Mus\u00e9um National d\u2019Histoire Naturelle, n. s. 6(2): 187\u2013353."},{"key":"204580_B2","unstructured":"AVMA [American Veterinary Medical Association] (2020) AVMA Guidelines for the Euthanasia of Animals, 2020 edn. [accessed 28 Feb 2026]"},{"key":"204580_B3","unstructured":"Boutin C (2001) Cambodge. In: Juberthie C, Decu V (Eds) Encyclopaedia Biospeologica. Volume 3. Soci\u00e9t\u00e9 Internationale de Biosp\u00e9ologie, Moulis, 1755\u20131762."},{"key":"204580_B4","doi-asserted-by":"publisher","DOI":"10.1641\/0006-3568(2006)56[733:LKOSAI]2.0.CO;2"},{"key":"204580_B5","doi-asserted-by":"publisher","DOI":"10.1111\/j.1096-3642.1993.tb00305.x"},{"key":"204580_B6","doi-asserted-by":"publisher","DOI":"10.3897\/zookeys.510.8640"},{"key":"204580_B7","unstructured":"Golovatch SI, Geoffroy JJ, Mauri\u00e8s JP, VandenSpiegel D (2007a) Review of the millipede genus Glyphiulus Gervais, 1847, with descriptions of new species from Southeast Asia (Diplopoda, Spirostreptida, Cambalopsidae). Part 1: the granulatus-group. Zoosystema 29(1): 7\u201349."},{"key":"204580_B8","unstructured":"Golovatch SI, Geoffroy JJ, Mauri\u00e8s JP, VandenSpiegel D (2007b) Review of the millipede genus Glyphiulus Gervais, 1847, with descriptions of new species from Southeast Asia (Diplopoda, Spirostreptida, Cambalopsidae). Part 2: the javanicus-group. Zoosystema 29(3): 417\u2013456."},{"key":"204580_B9","doi-asserted-by":"publisher","DOI":"10.5252\/z2009n1a5"},{"key":"204580_B10","doi-asserted-by":"publisher","DOI":"10.11646\/zootaxa.2940.1.1"},{"key":"204580_B11","doi-asserted-by":"publisher","DOI":"10.5962\/bhl.part.91419"},{"key":"204580_B12","doi-asserted-by":"publisher","DOI":"10.11646\/zootaxa.5383.4.5"},{"key":"204580_B13","unstructured":"Jeekel CAW (2004) A bibliographic catalogue of the \u201cCambaloidea\u201d (Diplopoda, Spirostreptida). Myriapod Memoranda 7: 43\u2013109."},{"key":"204580_B14","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msy096"},{"key":"204580_B15","doi-asserted-by":"publisher","DOI":"10.3897\/zookeys.72.744"},{"key":"204580_B16","doi-asserted-by":"publisher","DOI":"10.3897\/zookeys.131.1921"},{"key":"204580_B17","doi-asserted-by":"publisher","DOI":"10.3897\/zookeys.435.8286"},{"key":"204580_B18","doi-asserted-by":"publisher","DOI":"10.11646\/zootaxa.3973.1.7"},{"key":"204580_B19","doi-asserted-by":"publisher","DOI":"10.5852\/ejt.2016.195"},{"key":"204580_B20","doi-asserted-by":"publisher","DOI":"10.3897\/zookeys.760.24837"},{"key":"204580_B21","doi-asserted-by":"publisher","DOI":"10.3897\/zookeys.938.51234"},{"key":"204580_B22","doi-asserted-by":"publisher","DOI":"10.3897\/zookeys.925.49953"},{"key":"204580_B23","doi-asserted-by":"publisher","DOI":"10.3897\/zookeys.1084.78744"},{"key":"204580_B24","doi-asserted-by":"publisher","DOI":"10.58837\/tnh.23.7.258815"},{"key":"204580_B25","doi-asserted-by":"publisher","DOI":"10.26107\/RBZ-2024-0003"},{"key":"204580_B26","doi-asserted-by":"publisher","DOI":"10.3897\/zookeys.1251.158776"},{"key":"204580_B27","doi-asserted-by":"publisher","DOI":"10.5252\/zoosystema2025v47a5"},{"key":"204580_B28","doi-asserted-by":"publisher","DOI":"10.3897\/zookeys.1238.147550"},{"key":"204580_B29","doi-asserted-by":"publisher","DOI":"10.58837\/tnh.25.8.266228"},{"key":"204580_B30","doi-asserted-by":"publisher","DOI":"10.11646\/zootaxa.5777.3.10"},{"key":"204580_B31","doi-asserted-by":"publisher","DOI":"10.3897\/zookeys.1282.186746"},{"key":"204580_B32","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0196554"},{"key":"204580_B33","unstructured":"Mauri\u00e8s JP (1970) Examen des types des genres Cambalomorpha et Cambalopsis Pocock, 1895. Essai de classification des Glyphiulinae Verhoeff, 1936 (Diplopoda, Cambalidea). Bulletin du Mus\u00e9um National \u010fHistoire Naturelle, 2e s\u00e9rie 42(3): 509\u2013519."},{"key":"204580_B34","doi-asserted-by":"publisher","DOI":"10.5962\/p.286001"},{"key":"204580_B35","doi-asserted-by":"publisher","DOI":"10.5852\/ejt.2025.987.2871"},{"key":"204580_B36","doi-asserted-by":"publisher","DOI":"10.3897\/zookeys.1145.98704"},{"key":"204580_B37","doi-asserted-by":"publisher","DOI":"10.11646\/zootaxa.5649.1.1"},{"key":"204580_B38","doi-asserted-by":"publisher","DOI":"10.1155\/jzs\/6387574"},{"key":"204580_B39","doi-asserted-by":"publisher","DOI":"10.26515\/rzsi\/v25\/i2\/1923\/162702"},{"key":"204580_B40","doi-asserted-by":"publisher","DOI":"10.3897\/zookeys.761.24214"},{"key":"204580_B41","doi-asserted-by":"publisher","DOI":"10.3897\/zookeys.996.57411"},{"key":"204580_B42","doi-asserted-by":"publisher","DOI":"10.58837\/tnh.23.7.258821"},{"key":"204580_B43","doi-asserted-by":"publisher","DOI":"10.15560\/14.6.1131"},{"key":"204580_B44","doi-asserted-by":"publisher","DOI":"10.1186\/s13358-025-00353-w"}],"container-title":["ZooKeys"],"original-title":[],"link":[{"URL":"https:\/\/zookeys.pensoft.net\/article\/204580\/download\/pdf\/","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/zookeys.pensoft.net\/article\/204580\/download\/xml\/","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/zookeys.pensoft.net\/article_preview.php?id=204580&skip_redirect=1","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T08:28:20Z","timestamp":1786696100000},"score":1,"resource":{"primary":{"URL":"https:\/\/zookeys.pensoft.net\/article\/204580\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,14]]},"references-count":44,"URL":"https:\/\/doi.org\/10.3897\/zookeys.1289.204580","relation":{},"ISSN":["1313-2970","1313-2989"],"issn-type":[{"value":"1313-2970","type":"electronic"},{"value":"1313-2989","type":"print"}],"subject":[],"published":{"date-parts":[[2026,8,14]]}}}