{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T09:03:27Z","timestamp":1791277407475,"version":"4.1.0"},"reference-count":21,"publisher":"The Journal of Rheumatology","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Rheumatol"],"published-print":{"date-parts":[[2009,6]]},"abstract":"<jats:sec><jats:title>Objective.<\/jats:title><jats:p>Numerous dominant human homolog of progressive ankylosis (<jats:italic>ANKH<\/jats:italic>) mutations have been identified in familial calcium pyrophosphate dihydrate crystal deposition disease (CPPDD). Due to the dominant nature of these mutations, we investigated whether ANKH interacts with other proteins; and if so, whether any CPPDD-associated <jats:italic>ANKH<\/jats:italic> mutation might disrupt such protein interactions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods.<\/jats:title><jats:p>Stable ATDC5 <jats:italic>ANKH wt-<\/jats:italic> and <jats:italic>ANKH M48T-<\/jats:italic>transfectants were generated. Lysates from these transfectants were used to identify candidate protein interaction with ANKH by coimmunoprecipitation followed by Western blot analysis. The effect of high phosphate on the expression of genes involved in modulating Pi (inorganic phosphate)\/PPi (inorganic pyrophosphate) homeostasis in these transfectants was assessed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results.<\/jats:title><jats:p>We showed that ANKH protein associates with the sodium\/phosphate cotransporter PiT-1, and that ANKH M48T mutant protein failed to interact with PiT-1. We also showed that upon high phosphate treatment, the normally coordinated upregulation of endogenous <jats:italic>Ank<\/jats:italic> and <jats:italic>PiT1<\/jats:italic> transcript expression was disrupted in <jats:italic>ANKH M48T<\/jats:italic> transfectants.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our results suggested that there is a coordinated interrelationship between 2 key participants of Pi and PPi metabolism, ANKH and PiT-1.<\/jats:p><\/jats:sec>","DOI":"10.3899\/jrheum.081118","type":"journal-article","created":{"date-parts":[[2009,4,16]],"date-time":"2009-04-16T01:05:56Z","timestamp":1239843956000},"page":"1265-1272","source":"Crossref","is-referenced-by-count":19,"title":["The CPPDD-Associated <i>ANKH M48T<\/i> Mutation Interrupts the Interaction of ANKH with the Sodium\/Phosphate Cotransporter PiT-1"],"prefix":"10.3899","volume":"36","author":[{"given":"JOHN","family":"WANG","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"HING WO","family":"TSUI","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"FRANK","family":"BEIER","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"FLORENCE W.L.","family":"TSUI","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2262","published-online":{"date-parts":[[2009,4,15]]},"reference":[{"key":"2018112909194960000_36.6.1265.1","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1126\/science.289.5477.265","article-title":"Role of the mouse ank gene in control of tissue calcification and arthritis","volume":"289","author":"Ho","year":"2000","journal-title":"Science"},{"key":"2018112909194960000_36.6.1265.2","doi-asserted-by":"publisher","DOI":"10.1038\/88236"},{"key":"2018112909194960000_36.6.1265.3","doi-asserted-by":"publisher","DOI":"10.1086\/320612"},{"key":"2018112909194960000_36.6.1265.4","doi-asserted-by":"publisher","DOI":"10.1086\/343054"},{"key":"2018112909194960000_36.6.1265.5","doi-asserted-by":"publisher","DOI":"10.1086\/343053"},{"key":"2018112909194960000_36.6.1265.6","doi-asserted-by":"publisher","DOI":"10.1002\/art.11133"},{"key":"2018112909194960000_36.6.1265.7","doi-asserted-by":"publisher","DOI":"10.1002\/art.20978"},{"key":"2018112909194960000_36.6.1265.8","doi-asserted-by":"publisher","DOI":"10.1111\/j.0013-9580.2004.19504.x"},{"key":"2018112909194960000_36.6.1265.9","doi-asserted-by":"publisher","DOI":"10.1086\/509881"},{"key":"2018112909194960000_36.6.1265.10","doi-asserted-by":"publisher","DOI":"10.1186\/ar2072"},{"key":"2018112909194960000_36.6.1265.11","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1276205"},{"key":"2018112909194960000_36.6.1265.12","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.25.1.312-323.2005"},{"key":"2018112909194960000_36.6.1265.13","doi-asserted-by":"publisher","DOI":"10.1007\/s00223-005-0184-7"},{"key":"2018112909194960000_36.6.1265.14","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M701116200"},{"key":"2018112909194960000_36.6.1265.15","first-page":"25","article-title":"The ANKH \u0394E490 mutation in calcium pyrophosphate dihydrate crystal deposition disease (CPPDD) affects tissue non-specific alkaline phosphatase (TNAP) activities","volume":"2","author":"Wang","year":"2008","journal-title":"Open Rheum J"},{"key":"2018112909194960000_36.6.1265.16","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.1997.12.8.1174"},{"key":"2018112909194960000_36.6.1265.17","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M311427200"},{"key":"2018112909194960000_36.6.1265.18","doi-asserted-by":"publisher","DOI":"10.1097\/01.LAB.0000075640.49586.E6"},{"key":"2018112909194960000_36.6.1265.19","doi-asserted-by":"publisher","DOI":"10.1006\/abio.2000.4753"},{"key":"2018112909194960000_36.6.1265.20","doi-asserted-by":"publisher","DOI":"10.1089\/ten.2006.0431"},{"key":"2018112909194960000_36.6.1265.21","doi-asserted-by":"crossref","first-page":"217","DOI":"10.3109\/03009747709095453","article-title":"Hereditary diffuse articular chondrocalcinosis. Dominant manifestation without close linkage with the HLA system in a large pedigree","volume":"6","author":"Gaucher","year":"1977","journal-title":"Scand J Rheumatol"}],"container-title":["The Journal of Rheumatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.3899\/jrheum.081118","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2018,11,29]],"date-time":"2018-11-29T17:31:44Z","timestamp":1543512704000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.jrheum.org\/lookup\/doi\/10.3899\/jrheum.081118"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,4,15]]},"references-count":21,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2009,6,8]]},"published-print":{"date-parts":[[2009,6]]}},"alternative-id":["10.3899\/jrheum.081118"],"URL":"https:\/\/doi.org\/10.3899\/jrheum.081118","relation":{},"ISSN":["0315-162X","1499-2752"],"issn-type":[{"value":"0315-162X","type":"print"},{"value":"1499-2752","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,4,15]]}}}