{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T01:09:19Z","timestamp":1781831359468,"version":"3.54.5"},"reference-count":31,"publisher":"The Journal of Rheumatology","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Rheumatol"],"published-print":{"date-parts":[[2011,1]]},"abstract":"<jats:sec><jats:title>Objective.<\/jats:title><jats:p>Enthesitis-related arthritis subtype of juvenile idiopathic arthritis (JIA-ERA) clinically resembles reactive arthritis (ReA). In patients with ReA the immune response is targeted at the outer membrane protein (OMP) of<jats:italic>Salmonella typhimurium<\/jats:italic>. We studied the immune response in JIA-ERA to<jats:italic>S. typhimurium<\/jats:italic>OMP.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods.<\/jats:title><jats:p>Synovial fluid mononuclear cells (SFMC) and peripheral blood mononuclear cells (PBMC) were isolated from blood and SF of patients with JIA-ERA. Lymphocyte transformation test was done with<jats:italic>S. typhimurium<\/jats:italic>OMP and crude bacterial lysates of<jats:italic>Yersinia enterocolitica<\/jats:italic>,<jats:italic>Shigella flexneri<\/jats:italic>, and<jats:italic>S. typhimurium<\/jats:italic>. IgG and IgA ELISA were performed in serum and SF using<jats:italic>S. typhimurium<\/jats:italic>OMP as antigen and compared with sera from healthy controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results.<\/jats:title><jats:p>In PBMC samples (n = 25) an antigen-specific proliferative response was seen in 13 patients and a cross-reactive response in 6. Among these 19 patients, 12 showed response to OMP. In SFMC (n = 15) antigen-specific responses were seen in 3 patients and cross-reactive responses in 9. Among these 12 patients, 11 showed response to OMP. The IgG and IgA anti-OMP antibody concentrations in serum and SF were similar in patients and controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion.<\/jats:title><jats:p>In JIA-ERA, OMP is the major antigenic target recognized by both SFMC and PBMC. Response to OMP is independent of specific bacterial response, suggesting that OMP is the immunodominant antigen. 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