{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T11:04:45Z","timestamp":1760267085464},"reference-count":30,"publisher":"The Journal of Rheumatology","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Rheumatol"],"published-print":{"date-parts":[[2011,6]]},"abstract":"<jats:sec><jats:title>Objective.<\/jats:title><jats:p>To analyze \u03b3\u03b4T cell subsets in peripheral blood (PB) and synovial fluid (SF) of patients with juvenile idiopathic arthritis (JIA), and to correlate \u03b3\u03b4T cell subsets with clinical characteristics.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods.<\/jats:title><jats:p>\u03b3\u03b4T cell subsets as percentages of CD3+ T cells in samples of PB (n = 25) and SF (n = 93) were analyzed by flow cytometry in 93 JIA patients. The percentage of V\u03b39+ \u03b3\u03b4T cells after 10 days of<jats:italic>in vitro<\/jats:italic>expansion with either interleukin 2 (IL-2) or isopentenyl pyrophosphate (IPP) plus IL-2 was determined.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results.<\/jats:title><jats:p>Both V\u03b41+ and V\u03b39+ \u03b3\u03b4T cell subsets were detected in SF of all patients, but only the percentage of V\u03b41+ cells was higher in SF compared to PB (p &lt; 0.01). The distribution of \u03b3\u03b4T cell subsets was similar in different JIA subgroups, whereas antinuclear antibody (ANA)-positive patients had a higher percentage of SF V\u03b41+ T cells than ANA-negative patients (p &lt; 0.01). The percentage of SF V\u03b41+ T cells was inversely associated with age at onset, recurrence of synovitis, and erythrocyte sedimentation rate; and that of SF V\u03b39+ T cells was inversely correlated with age at onset and was higher in patients who recovered from disease (n = 15). IPP-induced expansion of SF V\u03b39+ T cells correlated with disease remission, whereas the expansion of SF V\u03b39+ T cells in media with IL-2 alone was significantly greater in patients with uveitis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion.<\/jats:title><jats:p>The percentage of V\u03b41+ and V\u03b39+ \u03b3\u03b4T cells among the SF T cells and their ability to respond to IPP or IL-2 correlated with specific outcomes of JIA, suggesting their role in the immunopathogenesis of this disease.<\/jats:p><\/jats:sec>","DOI":"10.3899\/jrheum.100938","type":"journal-article","created":{"date-parts":[[2011,3,16]],"date-time":"2011-03-16T02:24:25Z","timestamp":1300242265000},"page":"1123-1129","source":"Crossref","is-referenced-by-count":22,"title":["\u03b3\u03b4T cells in Juvenile Idiopathic Arthritis: Higher Percentages of Synovial V\u03b41+ and V\u03b39+ T Cell Subsets Are Associated with Milder 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