{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T09:51:27Z","timestamp":1761126687525},"reference-count":43,"publisher":"The Journal of Rheumatology","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Rheumatol"],"published-print":{"date-parts":[[2016,1]]},"abstract":"<jats:sec><jats:title>Objective.<\/jats:title><jats:p>To identify differentially expressed microRNA (miRNA) in muscle biopsies (MBx) from 15 untreated children with juvenile dermatomyositis (JDM) compared with 5 controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods.<\/jats:title><jats:p>Following MBx miRNA profiling, differentially expressed miRNA and their protein targets were validated by quantitative real-time PCR (qRT-PCR) and immunological assay. The association of miRNA-10a and miRNA-10b with clinical data was evaluated, including Disease Activity Score (DAS), von Willebrand factor antigen (vWF:Ag), nailfold capillary end row loops, duration of untreated disease, and tumor necrosis factor (TNF)-\u03b1-308A allele.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results.<\/jats:title><jats:p>In JDM, 16\/362 miRNA were significantly differentially expressed [false discovery rate (FDR) &lt; 0.05]. Among these, miRNA-10a was the most downregulated miRNA in both FDR and ranking of fold change: miRNA-10a = \u22122.27-fold, miRNA-10b = \u22121.80-fold. Decreased miRNA-10a and miRNA-10b expressions were confirmed using qRT-PCR: \u22124.16 and \u22122.59 fold, respectively. The qRT-PCR documented that decreased miRNA-10a expression was related to increased vascular cell adhesion molecule 1 in 13 of these JDM cases (correlation \u22120.67, p = 0.012), unlike miRNA-10b data (not significant). Concurrent JDM plasma contained increased levels of interleukin (IL) 6 (p = 0.0363), IL-8 (p = 0.0005), TNF-\u03b1 (p = 0.0011), and monocyte chemoattractant proteins 1 (p = 0.0139). Decreased miRNA-10a, but not miRNA-10b, was associated with the TNF-\u03b1-308A allele (p = 0.015). In the 15 JDM, a trend of association of miRNA-10a (but not miRNA-10b) with vWF:Ag and DAS was observed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion.<\/jats:title><jats:p>MiRNA-10a downregulation is an important element in untreated JDM muscle pathophysiology. We speculate that muscle miRNA expression in adult dermatomyositis differs from muscle miRNA expression in untreated childhood JDM.<\/jats:p><\/jats:sec>","DOI":"10.3899\/jrheum.141474","type":"journal-article","created":{"date-parts":[[2015,12,2]],"date-time":"2015-12-02T03:04:06Z","timestamp":1449025446000},"page":"161-168","source":"Crossref","is-referenced-by-count":20,"title":["MicroRNA-10a Regulation of Proinflammatory Mediators: An Important Component of Untreated Juvenile Dermatomyositis"],"prefix":"10.3899","volume":"43","author":[{"given":"Dong","family":"Xu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chiang-Ching","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akadia","family":"Kachaochana","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabrielle A.","family":"Morgan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria F.","family":"Bonaldo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcelo B.","family":"Soares","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabricio","family":"Costa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"Sarwark","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simone T.","family":"Sredni","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lauren M.","family":"Pachman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2262","published-online":{"date-parts":[[2015,12,1]]},"reference":[{"key":"2018112909351262000_43.1.161.1","doi-asserted-by":"publisher","DOI":"10.1002\/art.11122"},{"key":"2018112909351262000_43.1.161.2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(08)60955-1"},{"key":"2018112909351262000_43.1.161.3","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197502132920706"},{"key":"2018112909351262000_43.1.161.4","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197502202920807"},{"key":"2018112909351262000_43.1.161.5","doi-asserted-by":"publisher","DOI":"10.1097\/00005792-196607000-00001"},{"key":"2018112909351262000_43.1.161.6","doi-asserted-by":"publisher","DOI":"10.1002\/art.23012"},{"key":"2018112909351262000_43.1.161.7","doi-asserted-by":"publisher","DOI":"10.1002\/art.10924"},{"key":"2018112909351262000_43.1.161.8","doi-asserted-by":"publisher","DOI":"10.1002\/acr.20280"},{"key":"2018112909351262000_43.1.161.9","doi-asserted-by":"publisher","DOI":"10.1002\/art.23299"},{"key":"2018112909351262000_43.1.161.10","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpeds.2005.10.032"},{"key":"2018112909351262000_43.1.161.11","doi-asserted-by":"publisher","DOI":"10.1016\/j.autrev.2009.02.026"},{"key":"2018112909351262000_43.1.161.12","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/ker379"},{"key":"2018112909351262000_43.1.161.13","doi-asserted-by":"publisher","DOI":"10.1016\/j.clim.2007.06.011"},{"key":"2018112909351262000_43.1.161.14","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.168.8.4154"},{"key":"2018112909351262000_43.1.161.15","doi-asserted-by":"crossref","first-page":"59","DOI":"10.2119\/2006-00085.Baechler","article-title":"An interferon signature in the peripheral blood of dermatomyositis patients is associated with disease activity","volume":"13","author":"Baechler","year":"2007","journal-title":"Mol Med"},{"key":"2018112909351262000_43.1.161.16","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(200010)43:10<2368::AID-ANR26>3.0.CO;2-8"},{"key":"2018112909351262000_43.1.161.17","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpeds.2010.04.034"},{"key":"2018112909351262000_43.1.161.18","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpeds.2011.05.015"},{"key":"2018112909351262000_43.1.161.19","doi-asserted-by":"publisher","DOI":"10.1136\/ard.2010.137968"},{"key":"2018112909351262000_43.1.161.20","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvn156"},{"key":"2018112909351262000_43.1.161.21","doi-asserted-by":"crossref","unstructured":"Giacomazzi G Sampaolesi M Quattrocelli M . Unconventional players on the striated muscle field: microRNAs, signaling pathways and epigenetic regulators. Curr Stem Cell Res Ther 2015 Jan 12 (E-pub ahead of print).","DOI":"10.2174\/1574888X10666150113111320"},{"key":"2018112909351262000_43.1.161.22","doi-asserted-by":"publisher","DOI":"10.1111\/sji.12261"},{"key":"2018112909351262000_43.1.161.23","doi-asserted-by":"publisher","DOI":"10.4161\/cc.7.5.5547"},{"key":"2018112909351262000_43.1.161.24","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/ehu346"},{"key":"2018112909351262000_43.1.161.25","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0708115104"},{"key":"2018112909351262000_43.1.161.26","doi-asserted-by":"publisher","DOI":"10.1002\/art.38292"},{"key":"2018112909351262000_43.1.161.27","doi-asserted-by":"publisher","DOI":"10.1002\/art.34606"},{"key":"2018112909351262000_43.1.161.28","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"2018112909351262000_43.1.161.29","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2172-9-43"},{"key":"2018112909351262000_43.1.161.30","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1002120107"},{"key":"2018112909351262000_43.1.161.31","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1684\/ejd.2013.1959","article-title":"Down-regulation of miR-223 contributes to the formation of Gottron\u2019s papules in dermatomyositis via the induction of PKCvarepsilon","volume":"23","author":"Inoue","year":"2013","journal-title":"Eur J Dermatol"},{"key":"2018112909351262000_43.1.161.32","doi-asserted-by":"publisher","DOI":"10.4161\/rna.8.5.16324"},{"key":"2018112909351262000_43.1.161.33","doi-asserted-by":"crossref","first-page":"695754","DOI":"10.1155\/2013\/695754","article-title":"MicroRNAs as haematopoiesis regulators","volume":"2013","author":"Undi","year":"2013","journal-title":"Adv Hematol"},{"key":"2018112909351262000_43.1.161.34","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1179\/1607845412Y.0000000047","article-title":"Megakaryocytic differentiation of CD133+ hematopoietic stem cells by down-regulation of microRNA-10a","volume":"18","author":"Zarif","year":"2013","journal-title":"Hematology"},{"key":"2018112909351262000_43.1.161.35","doi-asserted-by":"crossref","first-page":"259689","DOI":"10.1155\/2014\/259689","article-title":"MicroRNA profiling of activated and tolerogenic human dendritic cells","volume":"2014","author":"Stumpfova","year":"2014","journal-title":"Mediators Inflamm"},{"key":"2018112909351262000_43.1.161.36","doi-asserted-by":"publisher","DOI":"10.1002\/art.22558"},{"key":"2018112909351262000_43.1.161.37","doi-asserted-by":"publisher","DOI":"10.1002\/art.27529"},{"key":"2018112909351262000_43.1.161.38","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0103097"},{"key":"2018112909351262000_43.1.161.39","first-page":"320","article-title":"von Willebrand factor in juvenile dermatomyositis","volume":"22","author":"Bloom","year":"1995","journal-title":"J Rheumatol"},{"key":"2018112909351262000_43.1.161.40","first-page":"193","article-title":"Imperfect indications of disease activity in juvenile dermatomyositis","volume":"22","author":"Pachman","year":"1995","journal-title":"J Rheumatol"},{"key":"2018112909351262000_43.1.161.41","doi-asserted-by":"publisher","DOI":"10.1007\/s10067-004-1027-z"},{"key":"2018112909351262000_43.1.161.42","doi-asserted-by":"publisher","DOI":"10.1006\/clim.2001.5063"},{"key":"2018112909351262000_43.1.161.43","doi-asserted-by":"publisher","DOI":"10.1186\/1742-4933-10-11"}],"container-title":["The Journal of Rheumatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.3899\/jrheum.141474","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,9,12]],"date-time":"2020-09-12T04:34:10Z","timestamp":1599885250000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.jrheum.org\/lookup\/doi\/10.3899\/jrheum.141474"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,12,1]]},"references-count":43,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2016,1,1]]},"published-print":{"date-parts":[[2016,1]]}},"alternative-id":["10.3899\/jrheum.141474"],"URL":"https:\/\/doi.org\/10.3899\/jrheum.141474","relation":{},"ISSN":["0315-162X","1499-2752"],"issn-type":[{"value":"0315-162X","type":"print"},{"value":"1499-2752","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,12,1]]}}}