{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T16:56:33Z","timestamp":1780592193805,"version":"3.54.1"},"reference-count":33,"publisher":"The Journal of Rheumatology","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Rheumatol"],"published-print":{"date-parts":[[2017,11]]},"abstract":"<jats:sec><jats:title>Objective.<\/jats:title><jats:p>The aim of our study was to determine whether an automated capture and analysis system could detect differences in structure and function of sublingual microvessels in patients with systemic sclerosis (SSc) compared to healthy controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods.<\/jats:title><jats:p>Intravital microscopy of the sublingual microcirculation was automatically captured and analyzed in 40 patients with SSc and 10 age-matched healthy controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results.<\/jats:title><jats:p>Total and perfused microvascular density were lower in patients with SSc compared with controls (total microvascular density: 2471 \u00b1 134 <jats:italic>\u00b5<\/jats:italic>m\/mm<jats:sup>2<\/jats:sup> vs 3067 \u00b1 197 <jats:italic>\u00b5<\/jats:italic>m\/mm<jats:sup>2<\/jats:sup>, p = 0.020; perfused microvascular density: 1708 \u00b1 92 <jats:italic>\u00b5<\/jats:italic>m\/mm<jats:sup>2<\/jats:sup> vs 2192 \u00b1 144 <jats:italic>\u00b5<\/jats:italic>m\/mm<jats:sup>2<\/jats:sup>, p = 0.009). However, the relative percentage of perfused to total microvascular density was similar between SSc and controls (72 \u00b1 2% vs 71 \u00b1 2%, respectively, p = 0.429). Mean red blood cell (RBC) fraction, which indicates the longitudinal tube hematocrit of microvessel segments, was lower in patients with SSc compared with controls (69 \u00b1 1% vs 77 \u00b1 1%, respectively, p &lt; 0.001). Perfused boundary region (PBR), a marker of endothelial glycocalyx barrier properties, was higher in patients with SSc compared with controls (2.1 \u00b1 0.0 <jats:italic>\u00b5<\/jats:italic>m vs 1.9 \u00b1 0.0 <jats:italic>\u00b5<\/jats:italic>m, respectively, p = 0.012), suggestive of a dysfunctional glycocalyx. There was an inverse association of PBR with perfused microvascular density (r = \u22120.40, p = 0.004) and RBC fraction (r = \u22120.80, p &lt; 0.001).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion.<\/jats:title><jats:p>Our results indicate that automated capture and analysis of sublingual microvessel segments produces detailed, objective microvascular structural and functional data that have allowed us to distinguish patients with SSc from controls. These data suggest that microvascular structural and functional abnormalities present in patients with SSc could be at least partly due to a dysfunctional glycocalyx.<\/jats:p><\/jats:sec>","DOI":"10.3899\/jrheum.170120","type":"journal-article","created":{"date-parts":[[2017,9,15]],"date-time":"2017-09-15T20:20:13Z","timestamp":1505506813000},"page":"1603-1611","source":"Crossref","is-referenced-by-count":33,"title":["Automated Measurement of Microvascular Function Reveals Dysfunction in Systemic Sclerosis: A Cross-sectional Study"],"prefix":"10.3899","volume":"44","author":[{"given":"Daniel R.","family":"Machin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phillip E.","family":"Gates","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans","family":"Vink","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tracy M.","family":"Frech","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony J.","family":"Donato","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2262","published-online":{"date-parts":[[2017,9,15]]},"reference":[{"key":"2018112909373332000_44.11.1603.1","doi-asserted-by":"publisher","DOI":"10.1002\/art.11073"},{"key":"2018112909373332000_44.11.1603.2","doi-asserted-by":"publisher","DOI":"10.3899\/jrheum.111380"},{"key":"2018112909373332000_44.11.1603.3","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)16853-0"},{"key":"2018112909373332000_44.11.1603.4","doi-asserted-by":"publisher","DOI":"10.1002\/art.23555"},{"key":"2018112909373332000_44.11.1603.5","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.90553.2008"},{"key":"2018112909373332000_44.11.1603.6","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.mvr.2015.03.002","article-title":"New insights into systemic sclerosis related microcirculatory dysfunction by assessment of sublingual micr\\ocirculation and vascular glycocalyx layer. 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