{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T20:29:50Z","timestamp":1790281790942,"version":"4.1.0"},"reference-count":46,"publisher":"The Journal of Rheumatology","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Rheumatol"],"published-print":{"date-parts":[[2019,11]]},"abstract":"<jats:sec><jats:title>Objective.<\/jats:title><jats:p>To investigate microRNA (miRNA) that is potentially implicated in primary Sj\u00f6gren syndrome (pSS)\u2013related salivary hypofunction in labial salivary glands and to study miRNA-mediated mechanisms underlying oral dryness and altered rheology, focusing on the mucin O-glycosylation pathway.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods.<\/jats:title><jats:p>We performed miRNA expression profiling in minor salivary gland samples of patients with pSS presenting a different impairment in their unstimulated salivary flow rate. A computational <jats:italic>in silico<\/jats:italic> analysis was performed to identify genes and pathways that might be modulated by the deregulated miRNA that we had identified. To confirm <jats:italic>in silico<\/jats:italic> analysis, expression levels of genes encoding for glycosyltransferases and glycan-processing enzymes were investigated using Human Glycosylation-RT<jats:sup>2<\/jats:sup> Profiler PCR Array.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results.<\/jats:title><jats:p>Among 754 miRNA analyzed, we identified 126 miRNA that were significantly deregulated in pSS compared to controls, with a trend that was inversely proportional with the impairment of salivary flow rates. An <jats:italic>in silico<\/jats:italic> approach pinpointed that several upregulated miRNA in patients with pSS target important genes in the mucin O-glycosylation. We confirmed this prediction by quantitative real-time PCR, highlighting the downregulation of some glycosyltransferase and glycosidase genes in pSS samples compared to controls, such as <jats:italic>GALNT1<\/jats:italic>, responsible for mucin-7 glycosylation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion.<\/jats:title><jats:p>Collectively, our data suggest that the expression of different predicted miRNA-target genes in the mucin type O-glycan biosynthesis pathway is altered in pSS patients with low salivary flow and that the miRNA expression profile could influence the glycosidase expression levels and consequently the rheology in pSS.<\/jats:p><\/jats:sec>","DOI":"10.3899\/jrheum.180549","type":"journal-article","created":{"date-parts":[[2019,3,1]],"date-time":"2019-03-01T12:24:14Z","timestamp":1551443054000},"page":"1485-1494","source":"Crossref","is-referenced-by-count":12,"title":["MicroRNA-mediated Regulation of Mucin-type O-glycosylation Pathway: A Putative Mechanism 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