{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T10:24:46Z","timestamp":1770978286234,"version":"3.50.1"},"reference-count":36,"publisher":"Walter de Gruyter GmbH","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BM"],"published-print":{"date-parts":[[2011,3,1]]},"abstract":"<jats:p>The aim of the study was to evaluate the safety and persistence of selected <jats:italic>Lactobacillus<\/jats:italic> strains in the gastrointestinal\ntract (GIT) of healthy adult volunteers after oral consumption of high doses of lactobacilli to identify potential\ncandidates for probiotic and biotechnological applications. In the first phase of the study, nine individuals consumed\ncapsules containing <jats:italic>Lactobacillus gasseri<\/jats:italic> 177 and E16B7, <jats:italic>Lactobacillus acidophilus<\/jats:italic> 821-3, <jats:italic>Lactobacillus paracasei<\/jats:italic>\n317 and <jats:italic>Lactobacillus fermentum<\/jats:italic> 338-1-1 (each daily dose 1\u00d710<jats:sup>10<\/jats:sup> cfu) for 5 consecutive days. Data on gut health,\nblood parameters, and liver and kidney function were collected. The persistence of <jats:italic>Lactobacillus<\/jats:italic> strains was\nassessed by culturing combined with arbitrarily primed polymerase chain reaction (AP-PCR) and PCR-denaturing\ngradient gel electrophoresis (PCR-DGGE) on days 0, 5, 8, 10 and 20 from faecal samples. All strains survived\ngastrointestinal passage and were detected on the 5<jats:sup>th<\/jats:sup> day. <jats:italic>L. acidophilus<\/jats:italic> 821-3 was detected in four volunteers on\nthe 8<jats:sup>th<\/jats:sup> day (4.3 to 7.0 log<jats:sub>10<\/jats:sub> cfu\/g) and in two on the 10<jats:sup>th<\/jats:sup> day (8.3 and 3.9 log<jats:sub>10<\/jats:sub> cfu\/g, respectively). In the second\nphase of the study, five additional volunteers consumed <jats:italic>L. acidophilus<\/jats:italic> 821-3 (daily 1\u00d710<jats:sup>10<\/jats:sup> cfu) for 5 consecutive\ndays. The strain was subsequently detected in faeces of all individuals using real-time PCR on the 10<jats:sup>th<\/jats:sup> day (range\n4.6-6.7; median 6.0 log<jats:sub>10<\/jats:sub> cell\/g) in both phases of the study for at least 5 days after discontinuation of consumption.\nThe administration of high doses of different <jats:italic>Lactobacillus<\/jats:italic> strains did not result in any severe adverse effects in\nGIT and\/or abnormal values of blood indices. Thus, the strain <jats:italic>L. acidophilus<\/jats:italic> 821-3 is a promising candidate for\nprobiotic and biotechnological applications. Further studies will be performed to confirm the strain persistence\nand safety in a larger number of individuals.<\/jats:p>","DOI":"10.3920\/bm2010.0023","type":"journal-article","created":{"date-parts":[[2011,3,15]],"date-time":"2011-03-15T13:20:49Z","timestamp":1300195249000},"page":"79-90","source":"Crossref","is-referenced-by-count":22,"title":["Safety and persistence of orally administered human Lactobacillus sp. strains in healthy adults"],"prefix":"10.1163","volume":"2","author":[{"given":"P.","family":"H\u00fctt","sequence":"first","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine, University of Tartu, Ravila 19, 50411 Tartu, Estonia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"K\u00f5ll","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine, University of Tartu, Ravila 19, 50411 Tartu, Estonia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Stsepetova","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine, University of Tartu, Ravila 19, 50411 Tartu, Estonia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Alvarez","sequence":"additional","affiliation":[{"name":"Division of Clinical Immunology, Department of Laboratory Medicine, Karolinska Institutet at Karolinska University Hospital Huddinge, 141 86 Stockholm, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"M\u00e4ndar","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine, University of Tartu, Ravila 19, 50411 Tartu, Estonia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Krogh-Andersen","sequence":"additional","affiliation":[{"name":"Division of Clinical Immunology, Department of Laboratory Medicine, Karolinska Institutet at Karolinska University Hospital Huddinge, 141 86 Stockholm, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Marcotte","sequence":"additional","affiliation":[{"name":"Division of Clinical Immunology, Department of Laboratory Medicine, Karolinska Institutet at Karolinska University Hospital Huddinge, 141 86 Stockholm, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Hammarstr\u00f6m","sequence":"additional","affiliation":[{"name":"Division of Clinical Immunology, Department of Laboratory Medicine, Karolinska Institutet at Karolinska University Hospital Huddinge, 141 86 Stockholm, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Mikelsaar","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine, University of Tartu, Ravila 19, 50411 Tartu, 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