{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T13:16:54Z","timestamp":1780492614051,"version":"3.54.1"},"reference-count":39,"publisher":"Walter de Gruyter GmbH","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BM"],"published-print":{"date-parts":[[2015,12,1]]},"abstract":"<jats:p>The study of biofilm function <jats:italic>in vivo<\/jats:italic> in various niches of the gastrointestinal tract (GIT) is rather limited. It is more frequently used in <jats:italic>in vitro<\/jats:italic> approaches, as an alternative to the studies focused on formation mechanisms and function of biofilms, which do not represent the actual <jats:italic>in vivo<\/jats:italic> complexity of microbial structures. Additionally, <jats:italic>in vitro<\/jats:italic> tests can sometimes lead to unreliable results. The goal of this study was to develop a simple approach to detect bacterial populations, particularly <jats:italic>Lactobacillus<\/jats:italic> and <jats:italic>Bifidobacterium<\/jats:italic> in biofilms, <jats:italic>in vivo<\/jats:italic> by the fluorescent <jats:italic>in situ<\/jats:italic> hybridisation (FISH) method. We standardised a new Histo-FISH method based on specific fluorochrome labelling probes which are able to detect <jats:italic>Lactobacillus<\/jats:italic> spp. and <jats:italic>Bifidobacterium<\/jats:italic> spp. within biofilms on the mucosal surface of the GIT embedded in paraffin in histological slices. This method is also suitable for visualisation of bacterial populations in the GIT internal content. Depending on the labelling probes, the Histo-FISH method has the potential to detect other probiotic strains or pathogenic bacteria. This original approach permits us to analyse bacterial colonisation processes as well as biofilm formation in stomach and caecum of BALB\/c and germ-free mice.<\/jats:p>","DOI":"10.3920\/bm2015.0016","type":"journal-article","created":{"date-parts":[[2015,6,30]],"date-time":"2015-06-30T07:16:33Z","timestamp":1435648593000},"page":"899-908","source":"Crossref","is-referenced-by-count":8,"title":["Histo-FISH protocol to detect bacterial compositions and biofilms formation in vivo"],"prefix":"10.1163","volume":"6","author":[{"given":"M.","family":"Madar","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy in Kosice, Komenskeho 73, 04181 Kosice, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Slizova","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy in Kosice, Komenskeho 73, 04181 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