{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,3,6]],"date-time":"2025-03-06T23:39:58Z","timestamp":1741304398114,"version":"3.38.0"},"reference-count":14,"publisher":"Walter de Gruyter GmbH","issue":"2","license":[{"start":{"date-parts":[[2016,3,11]],"date-time":"2016-03-11T00:00:00Z","timestamp":1457654400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BM"],"published-print":{"date-parts":[[2016,3,11]]},"abstract":"<jats:p>An assay was developed that tested the ability of <jats:italic>Lactobacillus acidophilus<\/jats:italic> to outcompete a challenge of <jats:italic>Escherichia coli<\/jats:italic> in a mixed culture containing different test nutrients. Using this assay, addition of fructo-oligosaccharide to the media allowed <jats:italic>L. acidophilus<\/jats:italic> to outcompete a challenge of <jats:italic>E. coli<\/jats:italic>, whereas in a mixed culture without the prebiotic the trend was reversed. Growth curves generated for <jats:italic>E. coli<\/jats:italic> in a single culture showed that fructo-oligosaccharide did not affect growth, indicating that the carbohydrate was not toxic to <jats:italic>E. coli.<\/jats:italic> This indicates that fructo-oligosaccharides may increase the ability of beneficial microbes to outcompete a pathogenic challenge. These results were confirmed using a skin simulant model that incorporates growth of the organisms at an air-surface interface to mimic the vulvar environment. It is possible to use a co-culture assay as an <jats:italic>in vitro<\/jats:italic> screening tool to define nutrients that confer a competitive advantage to beneficial flora specific to the female urogenital tract.<\/jats:p>","DOI":"10.3920\/bm2015.0047","type":"journal-article","created":{"date-parts":[[2015,12,21]],"date-time":"2015-12-21T15:27:21Z","timestamp":1450711641000},"page":"299-304","source":"Crossref","is-referenced-by-count":2,"title":["In vitro evaluation of nutrients that selectively confer a competitive advantage to lactobacilli"],"prefix":"10.1163","volume":"7","author":[{"given":"R.A.","family":"Vongsa","sequence":"first","affiliation":[{"name":"Kimberly Clark Corporation, 2100 County Rd II, Neenah, WI 54946, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.A.","family":"Minerath","sequence":"additional","affiliation":[{"name":"Kimberly Clark Corporation, 2100 County Rd II, Neenah, WI 54946, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.A.","family":"Busch","sequence":"additional","affiliation":[{"name":"Kimberly Clark Corporation, 2100 County Rd II, Neenah, WI 54946, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Tan","sequence":"additional","affiliation":[{"name":"Kimberly Clark Corporation, 2100 County Rd II, Neenah, WI 54946, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.W.","family":"Koenig","sequence":"additional","affiliation":[{"name":"Kimberly Clark Corporation, 2100 County Rd II, Neenah, WI 54946, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","reference":[{"key":"atypb1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1332765100"},{"volume":"39","journal-title":"FEMS Micriobiology Ecology","first-page":"67","key":"atypb2"},{"volume":"10","journal-title":"Microbiologica","first-page":"377","key":"atypb3"},{"key":"atypb4","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(16)37989-4"},{"key":"atypb5","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.69.4.2217-2222.2003"},{"key":"atypb6","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.11.6531-6537.2005"},{"key":"atypb7","doi-asserted-by":"publisher","DOI":"10.1126\/science.1225152"},{"volume":"40","journal-title":"Scandinavian Journal of Infectious Diseases","first-page":"31","key":"atypb8"},{"key":"atypb9","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/73.2.437s"},{"volume":"6","journal-title":"Medscape Women\u2019s Health","first-page":"9","key":"atypb10"},{"key":"atypb11","doi-asserted-by":"crossref","first-page":"S1","DOI":"10.1017\/S0007114510003909","volume":"104","journal-title":"British Journal of Nutrition"},{"key":"atypb12","doi-asserted-by":"publisher","DOI":"10.1016\/j.anaerobe.2004.12.002"},{"key":"atypb13","doi-asserted-by":"publisher","DOI":"10.1159\/000084911"},{"key":"atypb14","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.26905-0"}],"container-title":["Beneficial Microbes"],"original-title":[],"link":[{"URL":"https:\/\/brill.com\/view\/journals\/bm\/7\/2\/article-p299_14.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/brill.com\/downloadpdf\/journals\/bm\/7\/2\/article-p299_14.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/brill.com\/downloadpdf\/journals\/bm\/7\/2\/article-p299_14.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,4,17]],"date-time":"2024-04-17T08:44:57Z","timestamp":1713343497000},"score":1,"resource":{"primary":{"URL":"https:\/\/brill.com\/view\/journals\/bm\/7\/2\/article-p299_14.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,3,11]]},"references-count":14,"journal-issue":{"issue":"2"},"URL":"https:\/\/doi.org\/10.3920\/bm2015.0047","relation":{},"ISSN":["1876-2883","1876-2891"],"issn-type":[{"type":"print","value":"1876-2883"},{"type":"electronic","value":"1876-2891"}],"subject":[],"published":{"date-parts":[[2016,3,11]]}}}