{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T09:20:51Z","timestamp":1770974451986,"version":"3.50.1"},"reference-count":59,"publisher":"Walter de Gruyter GmbH","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BM"],"published-print":{"date-parts":[[2018,2,27]]},"abstract":"<jats:p>Spermine (SPM) and its precursor putrescine (PUT), regulated by ornithine decarboxylase (ODC) and diamino-oxidase (DAO), are polyamines required for cell growth and proliferation. Only a few studies have investigated the anti-inflammatory and tumour inhibitory properties of probiotics on mucosal polyamine levels. We investigated the effects of a high concentration multistrain probiotic for human use on colonic polyamine biosynthesis in dogs. Histological sections (inflammatory bowel disease, n=10; polyposis, n=5) were assessed after receiving 112 to 225\u00d710<jats:sup>9<\/jats:sup> lyophilised bacteria daily for 60 days at baseline (T0) and 30 days after treatment end (T90). Histology scores, expression of PUT, SPM, ODC and DAO, and a clinical activity index (CIBDAI) were compared at T0 and T90. In polyps, cellular proliferation (Ki-67 expression), and apoptosis (caspase-3 protein expression) were also evaluated. After treatment, in inflammatory bowel disease significant decreases were observed for CIBDAI (<jats:italic>P<\/jats:italic>=0.006) and histology scores (<jats:italic>P<\/jats:italic>&lt;0.001); PUT, SPM and ODC expression increased (<jats:italic>P<\/jats:italic>&lt;0.01). In polyps, a significant decrease in polyamine levels, ODC activity, and Ki-67, and a significant increase in caspase-3 positivity and DAO expression (<jats:italic>P<\/jats:italic>=0.005) was noted. Our results suggest potential anti-proliferative and anti-inflammatory effects of the probiotic mixture in polyps and inflammation, associated with reduced mucosal infiltration and up-regulation of PUT, SPM, and ODC levels.<\/jats:p>","DOI":"10.3920\/bm2017.0024","type":"journal-article","created":{"date-parts":[[2017,10,12]],"date-time":"2017-10-12T10:34:47Z","timestamp":1507804487000},"page":"247-256","source":"Crossref","is-referenced-by-count":24,"title":["Effects of probiotic bacteria on mucosal polyamines levels in dogs with IBD and colonic polyps: a preliminary study"],"prefix":"10.1163","volume":"9","author":[{"given":"G.","family":"Rossi","sequence":"first","affiliation":[{"name":"School of Biosciences and Veterinary Medicine, University of Camerino, Via Circonvallazione 93\/95, 62024 Macerata (MC), Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Cerquetella","sequence":"additional","affiliation":[{"name":"School of Biosciences and Veterinary Medicine, University of Camerino, Via Circonvallazione 93\/95, 62024 Macerata (MC), Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Scarpona","sequence":"additional","affiliation":[{"name":"School of Biosciences and Veterinary Medicine, University of Camerino, Via Circonvallazione 93\/95, 62024 Macerata (MC), Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Pengo","sequence":"additional","affiliation":[{"name":"Clinic \u2018St. Antonio\u2019, Strada Statale 415, km 38,50, 26020 Madignano (CR), Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Fettucciari","sequence":"additional","affiliation":[{"name":"Department of Experimental Medicine, University of Perugia School of Medicine, Piazzale Lucio Severi 1-8, 06123 Perugia, 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