{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,3,7]],"date-time":"2025-03-07T00:08:47Z","timestamp":1741306127958,"version":"3.38.0"},"reference-count":19,"publisher":"Walter de Gruyter GmbH","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BM"],"published-print":{"date-parts":[[2017,10,13]]},"abstract":"To investigate the inhibitory effect of indigenous enterobacteria on pathogenic Escherichia coli<\/jats:italic>, a challenge trial with postweaning pigs was conducted. A pathogenic E. coli<\/jats:italic> strain was administered to all animals and their health was closely monitored thereafter. Faecal samples were taken from three healthy and three diarrhoeic animals. Samples were cultivated on MacConkey agar and isolates were subcultured. A soft agar overlay assay was used to determine the inhibitory activity of the isolates. A total of 1,173 enterobacterial isolates were screened for their ability to inhibit the E. coli<\/jats:italic> challenge strain. Colony forming units of enterobacteria on MacConkey agar were not different between healthy and diarrhoeic animals in the original samples. Furthermore, numbers of isolates per animal were also not significantly different between healthy (482 isolates) and diarrhoeic animals (691 isolates). A total of 43 isolates (3.7%) with inhibitory activity against the pathogenic E. coli<\/jats:italic> challenge strain were detected. All inhibitory isolates were identified as E. coli<\/jats:italic> via MALDI-TOF. The isolates belonged to the phylotypes A, C and E. Many isolates (67.4%) were commensal E. coli<\/jats:italic> without relevant porcine pathogenic factors, but toxin- and fimbrial genes (stx<\/jats:italic>2e, fae<\/jats:italic>, est<\/jats:italic>Ib, elt<\/jats:italic>1a, fas, fan<\/jats:italic>) were detected in 14 inhibitory isolates. Healthy animals showed significantly (P<\/jats:italic>=0.003) more inhibitory isolates (36 of 482 isolates; 7.5%) than diseased animals (7 of 691 isolates; 1.0%). There were no significant correlations regarding phylotype or pathogenic factors between healthy and diseased animals. This study has shown that a small proportion of indigenous E. coli<\/jats:italic> is able to inhibit in vitro<\/jats:italic> growth of a pathogenic E. coli<\/jats:italic> strain in pigs. Furthermore, healthy animals possess significantly more inhibitory E. coli<\/jats:italic> strains than diarrhoeic animals. The inhibition of pathogenic E. coli<\/jats:italic> by specific indigenous E. coli<\/jats:italic> strains may be an underlying principle for the containment of pathogenic E. coli<\/jats:italic> in pigs.<\/jats:p>","DOI":"10.3920\/bm2017.0040","type":"journal-article","created":{"date-parts":[[2017,10,3]],"date-time":"2017-10-03T08:55:42Z","timestamp":1507020942000},"page":"779-784","source":"Crossref","is-referenced-by-count":1,"title":["Protective effects of indigenous Escherichia coli against a pathogenic E. coli challenge strain in pigs"],"prefix":"10.1163","volume":"8","author":[{"given":"W.","family":"Vahjen","sequence":"first","affiliation":[{"name":"Institute of Animal Nutrition, Freie Universitaet Berlin, Koenigin-Luise-Str. 49, 14195 Berlin, Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Cuisiniere","sequence":"additional","affiliation":[{"name":"Ecolle de Biologie Industrielle, 13 Boulevard de L\u2019hautil, 95092 Cergy-Pontoise, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Zentek","sequence":"additional","affiliation":[{"name":"Institute of Animal Nutrition, Freie Universitaet Berlin, Koenigin-Luise-Str. 49, 14195 Berlin, Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","reference":[{"key":"atypb1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01688-12"},{"key":"atypb2","doi-asserted-by":"publisher","DOI":"10.1111\/1574-6941.12223"},{"volume":"47","journal-title":"Microbial Pathogens","first-page":"274","key":"atypb3"},{"key":"atypb5","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.66.10.4555-4558.2000"},{"key":"atypb6","doi-asserted-by":"publisher","DOI":"10.1080\/19490976.2015.1078051"},{"key":"atypb7","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2008.01.024"},{"key":"atypb8","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.28690-0"},{"key":"atypb9","doi-asserted-by":"publisher","DOI":"10.1139\/w06-058"},{"key":"atypb10","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2012.07.049"},{"key":"atypb11","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2567.2012.03616.x"},{"key":"atypb12","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0053957"},{"key":"atypb13","doi-asserted-by":"publisher","DOI":"10.1093\/femspd\/ftw051"},{"key":"atypb14","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-016-0835-z"},{"volume":"2","journal-title":"Medizinische Klinik","first-page":"29","key":"atypb15"},{"key":"atypb16","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.0050177"},{"key":"atypb17","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v22.i24.5505"},{"key":"atypb18","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003730"},{"key":"atypb19","doi-asserted-by":"publisher","DOI":"10.1017\/S0022172400021653"},{"key":"atypb20","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2011.09.012"}],"container-title":["Beneficial Microbes"],"original-title":[],"link":[{"URL":"https:\/\/brill.com\/view\/journals\/bm\/8\/5\/article-p779_11.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/brill.com\/downloadpdf\/journals\/bm\/8\/5\/article-p779_11.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/brill.com\/downloadpdf\/journals\/bm\/8\/5\/article-p779_11.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,4,18]],"date-time":"2024-04-18T08:03:48Z","timestamp":1713427428000},"score":1,"resource":{"primary":{"URL":"https:\/\/brill.com\/view\/journals\/bm\/8\/5\/article-p779_11.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,10,13]]},"references-count":19,"journal-issue":{"issue":"5"},"URL":"https:\/\/doi.org\/10.3920\/bm2017.0040","relation":{},"ISSN":["1876-2883","1876-2891"],"issn-type":[{"type":"print","value":"1876-2883"},{"type":"electronic","value":"1876-2891"}],"subject":[],"published":{"date-parts":[[2017,10,13]]}}}