{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,11]],"date-time":"2025-12-11T20:48:52Z","timestamp":1765486132772,"version":"3.41.0"},"reference-count":34,"publisher":"Walter de Gruyter GmbH","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BM"],"published-print":{"date-parts":[[2018,1,29]]},"abstract":"<jats:p><jats:italic>Streptococcus thermophilus<\/jats:italic>YIT 2001 (ST-1), a lactic acid bacterial strain, was shown to have inhibitory effects on the oxidation of low-density lipoprotein (LDL) and the development of aortic fatty lesions in an animal model, and lower the serum levels of malondialdehyde-modified LDL, an oxidative modification product of LDL, in a clinical trial. This study aimed to identify the intracellular active component of ST-1 associated with anti-oxidative activity against LDL oxidation. High-performance liquid chromatography-electrospray ionisation mass spectrometry analysis after fractionation of the cellular extract by reversed-phase chromatography demonstrated that the active fraction contained reduced glutathione (GSH). GSH showed anti-oxidative activity in a dose-dependent manner, while this activity disappeared following thiol derivatisation. ST-1 had the strongest anti-oxidative activity against LDL oxidation and the highest level of intracellular GSH among five strains of<jats:italic>S. thermophilus<\/jats:italic>. In addition, the anti-oxidative activity of ST-1 after thiol derivatisation decreased by about half, which was similar to that of three other strains containing poor or no intracellular GSH or thiol components. Moreover, anti-oxidative activity against LDL oxidation was observed in hyperlipidaemic hamsters fed with high GSH ST-1 cells but not in those given low GSH cells. These findings suggest that intracellular GSH in ST-1 may provide beneficial effects via anti-oxidative activity against LDL oxidation and excess oxidative stress in the blood.<\/jats:p>","DOI":"10.3920\/bm2017.0065","type":"journal-article","created":{"date-parts":[[2017,10,25]],"date-time":"2017-10-25T07:54:08Z","timestamp":1508918048000},"page":"143-152","source":"Crossref","is-referenced-by-count":10,"title":["Intracellular GSH of Streptococcus thermophilus shows anti-oxidative activity against low-density lipoprotein oxidation in vitro and in a hyperlipidaemic hamster model"],"prefix":"10.1163","volume":"9","author":[{"given":"S.","family":"Kusuhara","sequence":"first","affiliation":[{"name":"Yakult Central Institute, 5-11 Izumi, Kunitachi-shi, Tokyo 186-8650, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Ito","sequence":"additional","affiliation":[{"name":"Yakult Central Institute, 5-11 Izumi, Kunitachi-shi, Tokyo 186-8650, 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