{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T03:15:57Z","timestamp":1780456557230,"version":"3.54.1"},"reference-count":30,"publisher":"Walter de Gruyter GmbH","issue":"4","license":[{"start":{"date-parts":[[2019,4,19]],"date-time":"2019-04-19T00:00:00Z","timestamp":1555632000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BM"],"published-print":{"date-parts":[[2019,4,19]]},"abstract":"<jats:p>Probiotics should bring \u2018balance\u2019 to the intestinal microbiota by stimulating beneficial bacteria, whilst mitigating adverse ones. Balance can also be interpreted as high alpha-diversity. Contrary, <jats:italic>Escherichia coli<\/jats:italic> is often regarded as an adverse component of the resident intestinal microbiota. The aim of the present study was to implement a mouse model for <jats:italic>in vivo<\/jats:italic> screening of <jats:italic>Lactobacillus<\/jats:italic>-strains for ability to increase gut-microbiota diversity and to mitigate <jats:italic>E. coli<\/jats:italic>. Mice were divided into six groups, two dietary control-groups and four groups administered strains of <jats:italic>Lactobacillus fermentum<\/jats:italic> and\/or <jats:italic>Lactobacillus plantarum<\/jats:italic>. All animals were pre-treated with antibiotics, and <jats:italic>E. coli<\/jats:italic> in order to equalise the microbiota from the start. After 7 weeks of <jats:italic>Lactobacillus<\/jats:italic> administration, the animals were sacrificed: DNA was extracted from caecum tissue, and the microbiota composition was analysed with terminal restriction fragment length polymorphism (T-RFLP) and 16S rRNA gene sequencing. The diversity of the caecal microbiota decreased when the dietary carbohydrate source was limited to corn starch. Conversely, the diversity was restored by <jats:italic>Lactobacillus<\/jats:italic>-supplements. The tested combinations of two <jats:italic>Lactobacillus<\/jats:italic> strains exerted different influences, not only on the taxonomic level, but also on the inferred microbiome functions. The mixture of <jats:italic>L. fermentum<\/jats:italic> GOS47 and <jats:italic>L. fermentum<\/jats:italic> GOS1 showed potential for anti-inflammatory activity and short chain fatty acid production. On the other hand, co-administration of <jats:italic>L. fermentum<\/jats:italic> GOS57 and <jats:italic>L. plantarum<\/jats:italic> GOS42 significantly decreased the viable count of <jats:italic>Enterobacteriaceae<\/jats:italic>. These results warrant further investigation of the tested strains as candidates for probiotics. Furthermore, the findings demonstrated that the current experimental animal model is suitable for <jats:italic>in vivo<\/jats:italic> studies of the effect of bacterial supplements on the gut-microbiota.<\/jats:p>","DOI":"10.3920\/bm2018.0074","type":"journal-article","created":{"date-parts":[[2019,4,8]],"date-time":"2019-04-08T11:22:46Z","timestamp":1554722566000},"page":"413-424","source":"Crossref","is-referenced-by-count":37,"title":["Lactobacillus fermentum and Lactobacillus plantarum increased gut microbiota diversity and functionality, and mitigated Enterobacteriaceae, in a mouse model"],"prefix":"10.1163","volume":"10","author":[{"given":"C.","family":"Linninge","sequence":"first","affiliation":[{"name":"Department of Food Technology, Engineering and Nutrition, Lund University, 221 00 Lund, Sweden."},{"name":"BioGaia AB, Mobilv\u00e4gen 10, 22362 Lund, Sweden."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Clinical and Experimental Medicine, Link\u00f6ping University, 582 25 Link\u00f6ping, Sweden."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.I.","family":"Bahl","sequence":"additional","affiliation":[{"name":"National Food Institute, Technical University of Denmark, Kgs. Lyngby, Denmark."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Ahrn\u00e9","sequence":"additional","affiliation":[{"name":"Department of Food Technology, Engineering and Nutrition, Lund University, 221 00 Lund, Sweden."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Molin","sequence":"additional","affiliation":[{"name":"Department of Food Technology, Engineering and Nutrition, Lund University, 221 00 Lund, Sweden."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","reference":[{"key":"atypb1","doi-asserted-by":"crossref","first-page":"434","DOI":"10.1016\/j.jaci.2011.10.025","volume":"129","journal-title":"Journal of Allergy and Clinical Immunology"},{"key":"atypb2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1002358"},{"key":"atypb3","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3869"},{"key":"atypb4","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.f.303"},{"key":"atypb5","unstructured":"Dietary heme induces gut dysbiosis, aggravates colitis, and potentiates the development of adenomas in miceFrontiers in Microbiology8"},{"key":"atypb6","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.03006-05"},{"key":"atypb7","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v18.i30.4012"},{"key":"atypb8","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.1989.tb05105.x"},{"key":"atypb10","doi-asserted-by":"publisher","DOI":"10.1016\/S0891-5520(05)70373-1"},{"key":"atypb11","doi-asserted-by":"crossref","first-page":"674","DOI":"10.1111\/j.1399-3038.2012.01328.x","volume":"23","journal-title":"Pediatric Allergy and Immunology"},{"key":"atypb12","doi-asserted-by":"publisher","DOI":"10.1016\/j.atherosclerosis.2009.06.019"},{"key":"atypb13","doi-asserted-by":"publisher","DOI":"10.1203\/PDR.0b013e318225f765"},{"key":"atypb14","doi-asserted-by":"publisher","DOI":"10.1017\/S0007114511001036"},{"key":"atypb15","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.2676"},{"key":"atypb16","first-page":"616","volume":"19","journal-title":"Journal of Microbiology and Biotechnology"},{"key":"atypb17","doi-asserted-by":"publisher","DOI":"10.1007\/s10482-014-0312-3"},{"key":"atypb18","doi-asserted-by":"publisher","DOI":"10.4291\/wjgp.v9.i1.18"},{"key":"atypb19","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/08910600902815561","volume":"21","journal-title":"Microbial Ecology in Health and Disease"},{"key":"atypb20","unstructured":"Lactobacillus plantarum: the role in foods and in human health353393"},{"key":"atypb21","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.1993.tb03031.x"},{"key":"atypb22","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(93)90485-Y"},{"key":"atypb23","doi-asserted-by":"publisher","DOI":"10.1111\/j.1572-0241.2000.02015.x"},{"key":"atypb24","first-page":"4","volume":"29","journal-title":"Animal Nutrition Health"},{"key":"atypb25","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu494"},{"key":"atypb26","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2006.100164"},{"key":"atypb27","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/20.Supplement_2.S149"},{"key":"atypb29","doi-asserted-by":"publisher","DOI":"10.1111\/jam.13339"},{"key":"atypb30","doi-asserted-by":"publisher","DOI":"10.1016\/S0891-5520(05)70371-8"},{"key":"atypb32","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2007.09.011"},{"key":"atypb33","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(01)65154-1"}],"container-title":["Beneficial Microbes"],"original-title":[],"link":[{"URL":"https:\/\/brill.com\/view\/journals\/bm\/10\/4\/article-p413_5.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/brill.com\/downloadpdf\/journals\/bm\/10\/4\/article-p413_5.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/brill.com\/downloadpdf\/journals\/bm\/10\/4\/article-p413_5.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,4,16]],"date-time":"2024-04-16T09:30:35Z","timestamp":1713259835000},"score":1,"resource":{"primary":{"URL":"https:\/\/brill.com\/view\/journals\/bm\/10\/4\/article-p413_5.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,4,19]]},"references-count":30,"journal-issue":{"issue":"4"},"URL":"https:\/\/doi.org\/10.3920\/bm2018.0074","relation":{},"ISSN":["1876-2883","1876-2891"],"issn-type":[{"value":"1876-2883","type":"print"},{"value":"1876-2891","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,4,19]]}}}