{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T17:02:55Z","timestamp":1779382975546,"version":"3.53.1"},"reference-count":70,"publisher":"Walter de Gruyter GmbH","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BM"],"published-print":{"date-parts":[[2020,9,1]]},"abstract":"<jats:p>Neonatal calf diarrhoea is one of the challenges faced by intensive farming, and probiotics are considered a promising approach to improve calves\u2019 health. The objective of this study was to evaluate the effect of potential probiotic lactobacilli on new-born dairy calves\u2019 growth, diarrhoea incidence, faecal score, cytokine expression in blood cells, immunoglobulin A (IgA) levels in plasma and faeces, and pathogen abundance in faeces. Two <jats:italic>in vivo<\/jats:italic> assays were conducted at the same farm in two annual calving seasons. Treated calves received one daily dose of the selected lactobacilli (<jats:italic>Lactobacillus reuteri<\/jats:italic> TP1.3B or <jats:italic>Lactobacillus johnsonii<\/jats:italic> TP1.6) for 10 consecutive days. A faecal score was recorded daily, average daily gain (ADG) was calculated, and blood and faeces samples were collected. Pathogen abundance was analysed by absolute qPCR in faeces using primers directed at <jats:italic>Salmonella enterica<\/jats:italic>, rotavirus, coronavirus, <jats:italic>Cryptosporidium parvum<\/jats:italic> and three <jats:italic>Escherichia coli<\/jats:italic> virulence genes (<jats:italic>eae, clpG<\/jats:italic> and <jats:italic>Stx1<\/jats:italic>). The faecal score was positively affected by the administration of both lactobacilli strains, and diarrhoea incidence was significantly lower in treated calves. No differences were found regarding ADG, cytokine expression, IgA levels and pathogen abundance. Our findings showed that oral administration of these strains could improve gastrointestinal health, but results could vary depending on the calving season, which may be related to pathogen seasonality and other environmental effects.<\/jats:p>","DOI":"10.3920\/bm2019.0167","type":"journal-article","created":{"date-parts":[[2020,9,2]],"date-time":"2020-09-02T16:22:55Z","timestamp":1599063775000},"page":"477-488","source":"Crossref","is-referenced-by-count":26,"title":["Effect of the administration of Lactobacillus spp. strains on neonatal diarrhoea, immune parameters and pathogen abundance in pre-weaned calves"],"prefix":"10.1163","volume":"11","author":[{"given":"S.","family":"Fern\u00e1ndez","sequence":"first","affiliation":[{"name":"Department of Microbiology, Instituto de Investigaciones Biol\u00f3gicas Clemente Estable, Av Italia 3318, Montevideo, 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