{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T23:26:34Z","timestamp":1780529194715,"version":"3.54.1"},"reference-count":4,"publisher":"Walter de Gruyter GmbH","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BM"],"published-print":{"date-parts":[[2020,12,2]]},"abstract":"<jats:p>Lipoteichoic acid (LTA) is a key component of the cell wall of most Gram-positive bacteria and plays many structural and functional roles. In probiotic lactobacilli, the function of LTA in mediating bacteria\/host cross-talk has been evidenced and it has been postulated that, owing to its anionic nature, LTA may play a role in toxic metal sequestration by these bacteria. However, studies on this last aspect employing strains unable to synthesise LTA are lacking. We have inactivated the LTA polymerase encoding gene <jats:italic>ltaS<\/jats:italic> in two different <jats:italic>Lactobacillus plantarum<\/jats:italic> strains. Analysis of LTA contents in wild-type and <jats:italic>ltaS<\/jats:italic> mutant strains corroborated the role of this gene as a major contributor to LTA synthesis in <jats:italic>L. plantarum<\/jats:italic>. The mutant strains displayed strain-dependent anomalous cell morphologies that resulted in elongated or irregular cells with aberrant septum formation. They also exhibited higher sensitivity to several stresses (osmotic and heat) and to antimicrobials that target the cell wall. The toxicity of inorganic [(Hg(II)] and organic mercury (methyl-Hg) was also increased upon <jats:italic>ltaS<\/jats:italic> mutation in a strain-dependent manner. However, the mutant strains showed 0 to 50% decrease in their capacity of Hg binding compared to their corresponding parental strains. This result suggests a partial contribution of LTA to Hg binding onto the cell surface that was dependent on the strain and the Hg form.<\/jats:p>","DOI":"10.3920\/bm2019.0184","type":"journal-article","created":{"date-parts":[[2020,11,16]],"date-time":"2020-11-16T11:47:41Z","timestamp":1605527261000},"page":"791-802","source":"Crossref","is-referenced-by-count":18,"title":["Lipoteichoic acid depletion in Lactobacillus impacts cell morphology and stress response but does not abolish mercury surface binding"],"prefix":"10.1163","volume":"11","author":[{"given":"C.","family":"Alc\u00e1ntara","sequence":"first","affiliation":[{"name":"Laboratorio de Bacterias L\u00e1cticas y Probi\u00f3ticos, Instituto de Agroqu\u00edmica y Tecnolog\u00eda de Alimentos (IATA-CSIC), Av. Agust\u00edn Escardino 7, 46980 Paterna, Valencia, Spain."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Crespo","sequence":"additional","affiliation":[{"name":"Laboratorio de Bacterias L\u00e1cticas y Probi\u00f3ticos, Instituto de Agroqu\u00edmica y Tecnolog\u00eda de Alimentos (IATA-CSIC), Av. Agust\u00edn Escardino 7, 46980 Paterna, Valencia, Spain."},{"name":"Laboratorio de Elementos Traza, Instituto de Agroqu\u00edmica y Tecnolog\u00eda de Alimentos (IATA-CSIC), Av. Agust\u00edn Escardino 7, 46980 Paterna, Valencia, Spain."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.L.S.","family":"Sol\u00eds","sequence":"additional","affiliation":[{"name":"Laboratorio de Bacterias L\u00e1cticas y Probi\u00f3ticos, Instituto de Agroqu\u00edmica y Tecnolog\u00eda de Alimentos (IATA-CSIC), Av. Agust\u00edn Escardino 7, 46980 Paterna, Valencia, Spain."},{"name":"Laboratorio de Elementos Traza, Instituto de Agroqu\u00edmica y Tecnolog\u00eda de Alimentos (IATA-CSIC), Av. Agust\u00edn Escardino 7, 46980 Paterna, Valencia, Spain."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"Devesa","sequence":"additional","affiliation":[{"name":"Laboratorio de Elementos Traza, Instituto de Agroqu\u00edmica y Tecnolog\u00eda de Alimentos (IATA-CSIC), Av. Agust\u00edn Escardino 7, 46980 Paterna, Valencia, Spain."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"V\u00e9lez","sequence":"additional","affiliation":[{"name":"Laboratorio de Elementos Traza, Instituto de Agroqu\u00edmica y Tecnolog\u00eda de Alimentos (IATA-CSIC), Av. Agust\u00edn Escardino 7, 46980 Paterna, Valencia, Spain."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"Monedero","sequence":"additional","affiliation":[{"name":"Laboratorio de Bacterias L\u00e1cticas y Probi\u00f3ticos, Instituto de Agroqu\u00edmica y Tecnolog\u00eda de Alimentos (IATA-CSIC), Av. Agust\u00edn Escardino 7, 46980 Paterna, Valencia, Spain."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Z\u00fa\u00f1iga","sequence":"additional","affiliation":[{"name":"Laboratorio de Bacterias L\u00e1cticas y Probi\u00f3ticos, Instituto de Agroqu\u00edmica y Tecnolog\u00eda de Alimentos (IATA-CSIC), Av. Agust\u00edn Escardino 7, 46980 Paterna, Valencia, Spain."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","reference":[{"key":"atypb18","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.63.6.2117-2123.1997"},{"key":"atypb24","first-page":"1","volume":"359","journal-title":"Hoppe-Seyler\u2019s Zeitschrift fur Physiologische Chemie"},{"key":"atypb25","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.67.4.686-723.2003"},{"key":"atypb48","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1861"}],"container-title":["Beneficial Microbes"],"original-title":[],"link":[{"URL":"https:\/\/brill.com\/view\/journals\/bm\/11\/8\/article-p791_6.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/brill.com\/downloadpdf\/journals\/bm\/11\/8\/article-p791_6.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/brill.com\/downloadpdf\/journals\/bm\/11\/8\/article-p791_6.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,4,17]],"date-time":"2024-04-17T08:41:52Z","timestamp":1713343312000},"score":1,"resource":{"primary":{"URL":"https:\/\/brill.com\/view\/journals\/bm\/11\/8\/article-p791_6.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,12,2]]},"references-count":4,"journal-issue":{"issue":"8"},"URL":"https:\/\/doi.org\/10.3920\/bm2019.0184","relation":{},"ISSN":["1876-2883","1876-2891"],"issn-type":[{"value":"1876-2883","type":"print"},{"value":"1876-2891","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,12,2]]}}}