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Estimates of the global burden of <jats:italic>S. pyogenes<\/jats:italic> related diseases revealed 616 million cases of pharyngitis, and at least 517,000 deaths due to severe invasive diseases and sequelae. Here we describe <jats:italic>Lactobacillus crispatus<\/jats:italic> DSM25988 that was identified among hundreds of <jats:italic>Lactobacillus<\/jats:italic> strains (referring to all organisms that were classified as <jats:italic>Lactobacillaceae<\/jats:italic> until 2020) showing ability to prevent adhesion of <jats:italic>S. pyogenes<\/jats:italic> to Detroit 562 cells, and to exhibit a masking and co-aggregating effect on <jats:italic>S. pyogenes in vitro<\/jats:italic>. <jats:italic>L. crispatus<\/jats:italic> DSM25988 also inhibits invasion of cultured human epithelial pharyngeal cells by <jats:italic>S. pyogenes.<\/jats:italic> Competitive binding to fibronectin might be involved in the inhibition process. Antiviral activity of the <jats:italic>L. crispatus<\/jats:italic> DSM25988 cells were identified in an <jats:italic>in vitro<\/jats:italic> cell model demonstrating that <jats:italic>L. crispatus<\/jats:italic> effectively excludes viruses from epithelial cells using SARS-CoV2 proteins as a model. This finding points to the potential of DSM25988 to protect cells from virus infection. Biological activity is retained in heat treated cells. The heat-treated <jats:italic>Lactobacillus<\/jats:italic> strain was further developed into functional throat lozenges, wherein its biological activity is stably maintained in the formulation. Lozenges containing <jats:italic>L. crispatus<\/jats:italic> DSM25988 underwent testing in an uncontrolled, prospective user study in 44 subjects with symptoms of sore throat for a period of up to 14 days. The study data shows promising safety and efficacy of the medical device when used against symptoms of sore throat like scratchy feeling, hoarse voice and swallowing pain.<\/jats:p>","DOI":"10.3920\/bm2021.0103","type":"journal-article","created":{"date-parts":[[2022,2,11]],"date-time":"2022-02-11T08:24:23Z","timestamp":1644567863000},"page":"83-94","source":"Crossref","is-referenced-by-count":3,"title":["Lactobacillus crispatus DSM25988 as novel bioactive agent to co-aggregate Streptococcus pyogenes and to exclude it by binding to human cells"],"prefix":"10.1163","volume":"13","author":[{"given":"A.","family":"Reiprich","sequence":"first","affiliation":[{"name":"Belano medical AG, Neuendorfstra\u00dfe 19, 16761 Hennigsdorf, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Skalden","sequence":"additional","affiliation":[{"name":"Belano medical AG, Neuendorfstra\u00dfe 19, 16761 Hennigsdorf, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Raab","sequence":"additional","affiliation":[{"name":"Belano medical AG, Neuendorfstra\u00dfe 19, 16761 Hennigsdorf, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Bolotina","sequence":"additional","affiliation":[{"name":"Belano medical AG, Neuendorfstra\u00dfe 19, 16761 Hennigsdorf, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Lang","sequence":"additional","affiliation":[{"name":"Belano medical AG, Neuendorfstra\u00dfe 19, 16761 Hennigsdorf, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","reference":[{"key":"atypb1","doi-asserted-by":"publisher","DOI":"10.1007\/s12602-014-9164-4"},{"key":"atypb2","doi-asserted-by":"publisher","DOI":"10.2903\/j.efsa.2008.923"},{"key":"atypb3","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2002.03180.x"},{"key":"atypb4","doi-asserted-by":"publisher","DOI":"10.1016\/j.micres.2013.09.015"},{"key":"atypb5","doi-asserted-by":"publisher","DOI":"10.1186\/s40795-018-0257-4"},{"key":"atypb6","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(05)70267-X"},{"key":"atypb7","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.01722"},{"key":"atypb8","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.6.1771-1776.1999"},{"key":"atypb9","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2006.11.007"},{"key":"atypb10","doi-asserted-by":"publisher","DOI":"10.1128\/iai.53.3.454-459.1986"},{"key":"atypb11","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-13-98"},{"key":"atypb12","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.1960.tb00188.x"},{"key":"atypb13","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/civ085"},{"key":"atypb14","doi-asserted-by":"publisher","DOI":"10.1021\/acsinfecdis.0c00073"},{"key":"atypb15","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/45.4_ts.502"},{"key":"atypb16","unstructured":"European Commission (EC), 2016. 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