{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T23:52:04Z","timestamp":1788306724091,"version":"build-2803163510"},"reference-count":38,"publisher":"Walter de Gruyter GmbH","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BM"],"published-print":{"date-parts":[[2023,4,14]]},"abstract":"<jats:p>Dysbiosis has been implicated in childhood obesity. Oral intake of fermented milk containing <jats:italic>Lacticaseibacillus casei<\/jats:italic> strain Shirota preserves gut microbiota (GM) diversity in children and adults. This study was a double-blind trial involving 37 overweight or obese children aged 6-10 years. Children were followed over a 6-week intervention period in which they received different fermented milk products containing <jats:italic>L. casei<\/jats:italic> Shirota: 10 in the first group received just <jats:italic>L. casei<\/jats:italic> Shirota; 13 received <jats:italic>L. casei<\/jats:italic> Shirota with 3 g\/day of inulin (<jats:italic>L. casei<\/jats:italic>+inulin); and 14 received <jats:italic>L. casei<\/jats:italic> Shirota with 3 g\/day of fructans from <jats:italic>Agave salmiana<\/jats:italic> (<jats:italic>L. casei<\/jats:italic>+fructans). Principal component analysis showed the relationship between microbial abundance, GM metabolites, and other obesity-related markers. Supplementation with probiotics and synbiotics improved the HDL-cholesterol levels of overweight and obese children, although no changes in body composition were detected. We observed an increase in butyrate or propionate concentrations in the <jats:italic>L. casei<\/jats:italic>+fructans group compared to the end of the intervention (<jats:italic>P<\/jats:italic>&lt;0.03). A diminished level of ANGPTL4 within the <jats:italic>L. casei<\/jats:italic>+fructans group (<jats:italic>P<\/jats:italic>=0.04) was also found, but no differences when lipopolysaccharide-binding protein was evaluated. The FFAR2+ cell frequency decreased between baseline and at the end of 6-week intervention in <jats:italic>L. casei<\/jats:italic>+inulin (<jats:italic>P<\/jats:italic>=0.02) and <jats:italic>L. casei<\/jats:italic>+fructans groups (<jats:italic>P<\/jats:italic>=0.04). In contrast, the percentage of CD14+FFAR3+ frequency increased in the same groups (<jats:italic>P<\/jats:italic>=0.04). The <jats:italic>L. casei<\/jats:italic> Shirota with inulin or fructans modulates GM, which improves the lipid profile and changes at a molecular level, such as expression of FFAR3 and FFAR2, ANGPTL4, propionate, and butyrate. It, therefore, could be considered an interesting therapeutic possibility for treating childhood overweight and obesity.<\/jats:p>\n<jats:p>The study was registered at <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/ClinicalTrials.gov\">ClinicalTrials.gov<\/jats:ext-link> (ID: NCT05423015).<\/jats:p>","DOI":"10.3920\/bm2022.0078","type":"journal-article","created":{"date-parts":[[2023,3,1]],"date-time":"2023-03-01T11:03:05Z","timestamp":1677668585000},"page":"153-164","source":"Crossref","is-referenced-by-count":6,"title":["Agave fructans enhance the effects of fermented milk products on obesity biomarkers: a randomised trial"],"prefix":"10.1163","volume":"14","author":[{"given":"M.","family":"Vega-C\u00e1rdenas","sequence":"first","affiliation":[{"name":"Research Centre for Health Sciences and Biomedicine, Autonomous University of San Luis Potos\u00ed (UASLP), Av. Sierra Leona 550, Lomas de San Luis 78210, San Luis Potos\u00ed, SLP, Mexico."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Mart\u00ednez-Gutierrez","sequence":"additional","affiliation":[{"name":"Research Centre for Health Sciences and Biomedicine, Autonomous University of San Luis Potos\u00ed (UASLP), Av. Sierra Leona 550, Lomas de San Luis 78210, San Luis Potos\u00ed, SLP, Mexico."},{"name":"Faculty of Chemical Sciences, UASLP, Av. Dr. Manuel Nava 6, Zona Universitaria 78210, San Luis Potos\u00ed, SLP, Mexico."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.E.","family":"Lara-Ram\u00edrez","sequence":"additional","affiliation":[{"name":"Zacatecas Biomedical Research Unit, Mexican Social Security Institute (IMSS), Alameda Trinidad Garc\u00eda de La Cadena 438, Zacatecas Centro 98000 Zacatecas, Zac, Mexico."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Reynaga-Hernandez","sequence":"additional","affiliation":[{"name":"Faculty of Chemical Sciences, UASLP, Av. Dr. Manuel Nava 6, Zona Universitaria 78210, San Luis Potos\u00ed, SLP, Mexico."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Ya\u00f1ez-Estrada","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, UASLP, Av. Dr. Manuel Nava 6, Zona Universitaria 78210, San Luis Potos\u00ed, SLP, Mexico."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Ratering","sequence":"additional","affiliation":[{"name":"Institute of Applied Microbiology, Justus Liebig University, Schubertstr. 81 35392, Giessen, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Schnell","sequence":"additional","affiliation":[{"name":"Institute of Applied Microbiology, Justus Liebig University, Schubertstr. 81 35392, Giessen, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.I.","family":"God\u00ednez-Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Desert Zones Research Institute, UASLP, De Altair 200, Col del Llano, 78377, San Luis Potos\u00ed, SLP, Mexico."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.M.","family":"Vargas-Morales","sequence":"additional","affiliation":[{"name":"Faculty of Chemical Sciences, UASLP, Av. 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