{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T13:46:03Z","timestamp":1791985563777,"version":"4.4.0"},"reference-count":24,"publisher":"SLACK, Inc.","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Orthopedics"],"published-print":{"date-parts":[[2026,9,1]]},"abstract":"\n Background:<\/jats:title>\n This exploratory laboratory study compared infrapatellar fat pad (IPFP)-derived cell concentrates obtained by non-enzymatic and collagenase-based processing with respect to cell yield, an adherent\/proliferative fraction, selected mesenchymal stromal cell-associated markers, clonogenicity, and qualitative differentiation.<\/jats:p>\n <\/jats:sec>\n \n Materials and Methods:<\/jats:title>\n \n Sixty independent patients were randomized to non-enzymatic or enzymatic processing (30 per group). Fresh concentrates were assessed for nucleated cell yield, attachment\/proliferation, colony formation, and selected markers. Archived group-level summaries were reassessed with two-sided Welch\n t<\/jats:italic>\n tests; marker comparisons were adjusted by the Benjamini-Hochberg procedure. Non-enzymatically isolated cells were culture-expanded for descriptive marker assessment and qualitative adipogenic, osteogenic, and chondrogenic staining.\n <\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n \n Enzymatic processing produced a higher nucleated cell yield than non-enzymatic processing (8.86 \u00b1 2.50 \u00d7 10\n 5<\/jats:sup>\n vs 1.19 \u00b1 1.17 \u00d7 105 cells\/g;\n P<\/jats:italic>\n < .001) and a higher adherent\/proliferative fraction (9.85% \u00b1 2.36% vs 3.25% \u00b1 1.23%;\n P<\/jats:italic>\n < .001). This fraction was not a live\/dead viability measurement. Seven of 8 marker-positive populations were higher after enzymatic processing following false-discovery-rate adjustment; CD105\n +<\/jats:sup>\n did not differ. Culture-stage marker findings and trilineage staining were descriptive and qualitative, respectively.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion:<\/jats:title>\n Non-enzymatic processing yielded heterogeneous IPFP-derived cell concentrates that could be culture expanded, but enzymatic processing produced higher cell yield and higher proportions of most measured marker-positive populations. The findings do not establish equivalence, clinical efficacy, complete International Society for Cell and Gene Therapy characterization, or quantitative viability.<\/jats:p>\n <\/jats:sec>","DOI":"10.3928\/01477447-20260910-01","type":"journal-article","created":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T19:40:10Z","timestamp":1790883610000},"source":"Crossref","is-referenced-by-count":0,"title":["Characterization of Infrapatellar Fat Pad-derived Cell Concentrates Obtained During Knee Arthroscopy: Nonenzymatic Versus Enzymatic Processing"],"prefix":"10.3928","volume":"49","author":[{"given":"Haobo","family":"Li","sequence":"first","affiliation":[{"name":"Department of Orthopedics, Shanghai Changzheng Hospital, Naval Medical University, Shanghai, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Orthopedics, Shanghai Changzheng Hospital, Naval Medical University, Shanghai, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Orthopedics, Shanghai Changzheng Hospital, Naval Medical University, Shanghai, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Orthopedics, Shanghai Changzheng Hospital, Naval Medical University, Shanghai, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Orthopedics, Shanghai Changzheng Hospital, Naval Medical University, Shanghai, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiwei","family":"Fu","sequence":"additional","affiliation":[{"name":"Department of Orthopedics, Shanghai Changzheng Hospital, Naval Medical University, Shanghai, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shu","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Orthopedics, Shanghai Changzheng Hospital, Naval Medical University, Shanghai, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiahua","family":"Shao","sequence":"additional","affiliation":[{"name":"Department of Orthopedics, Shanghai Changzheng Hospital, Naval Medical University, Shanghai, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiqin","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Orthopedics, Shanghai Changzheng Hospital, Naval Medical University, Shanghai, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qirong","family":"Qian","sequence":"additional","affiliation":[{"name":"Department of Orthopedics, Shanghai Changzheng Hospital, Naval Medical University, Shanghai, 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