{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,22]],"date-time":"2024-08-22T02:52:53Z","timestamp":1724295173615},"reference-count":37,"publisher":"SLACK, Inc.","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Pediatr Ophthalmol Strabismus"],"published-print":{"date-parts":[[2020,5]]},"abstract":"<jats:sec>\n            <jats:title>Purpose:<\/jats:title>\n            <jats:p>To measure the concentrations of growth factors in the aqueous humor of patients with congenital cataract and to investigate the biological effects of a selected cytokine (fibroblast growth factor 4 [FGF4]) on cell proliferation, migration, and transformation.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods:<\/jats:title>\n            <jats:p>In the aqueous humor obtained from 55 eyes with congenital cataract and 55 eyes with age-related cataract, 40 growth factors were screened and selected cytokines were confirmed with enzyme-linked immunosorbent assays. After the addition of various concentrations of FGF4 (0, 2.5, 15, or 50 ng\/mL) to the incubation medium, cellular functions were evaluated.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results:<\/jats:title>\n            <jats:p>The concentration of FGF4 was significantly higher in the aqueous humor of patients with congenital cataract than in that of patients with age-related cataract. The human SRA01\/04 lens epithelial cell line was treated with FGF4 and the cell proliferation increased significantly both dose- and time-dependently. The wound healing assay and Transwell migration assay revealed a significant increase in the migration capacity of the SRA01\/04 cell line treated with 15 or 50 ng\/mL of FGF4 compared with that of control cells. The intensity of immunofluorescent staining for \u03b1-smooth muscle actin increased significantly in the SRA01\/04 cell line when treated with FGF4. Cytoskeletal protein (F-actin) staining showed that changes of cell morphology were induced in primary lens epithelial cells by FGF4.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions:<\/jats:title>\n            <jats:p>This study provides a comprehensive profile of growth factors in congenital cataract. FGF4 induced cellular changes, and may have utility as a biomarker to predict the formation of visual axis opacification.<\/jats:p>\n            <jats:p>\n              <jats:bold>\n                [\n                <jats:italic>J Pediatr Ophthalmol Strabismus<\/jats:italic>\n                . 2020;57(3):159\u2013168.]\n              <\/jats:bold>\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.3928\/01913913-20200224-01","type":"journal-article","created":{"date-parts":[[2020,5,26]],"date-time":"2020-05-26T17:47:53Z","timestamp":1590515273000},"page":"159-168","source":"Crossref","is-referenced-by-count":2,"title":["Proliferative Status in the Aqueous Humor of Eyes With Congenital Cataract"],"prefix":"10.3928","volume":"57","author":[{"given":"Yinglei","family":"Zhang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dan","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiang","family":"Lu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Du","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi","family":"Lu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiangjia","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2308","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1001\/archophthalmol.2009.3"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/aos.13626"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0886-3350(01)00864-1"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.31729\/jnma.2807"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.3129\/i09-006"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2015.03.017"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00417-016-3313-2"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1042\/BCJ20160128"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/aos.13759"},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.25374"},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/eye.1995.137"},{"issue":"3","key":"e_1_3_1_13_2","first-page":"227","article-title":"Childhood blindness in the context of VISION 2020\u2014the right to sight","volume":"79","author":"Gilbert C","year":"2001","unstructured":"Gilbert C, Foster A. 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