{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T23:08:37Z","timestamp":1779318517422,"version":"3.51.4"},"reference-count":11,"publisher":"American Institute of Mathematical Sciences (AIMS)","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["AIMSMICRO"],"published-print":{"date-parts":[[2021]]},"abstract":"<abstract>\n<p>Last cholera epidemic has been recorded in Bangladesh between 1992\u20131993, while few sporadic localized outbreaks have been reported as recent as 2005. Serotype O1 of <italic>Vibrio cholera<\/italic> is considered as the principal causative agent which transmits through contaminated drinking water resulting that epidemic. Therefore, the objective of this research was to isolate <italic>V. cholera<\/italic> in 3 different water sources; River, pond and tube-well, in 5 different locations of Gazipur, Bangladesh, and to analyze their antibiogram study. A total of 45 water samples were randomly collected for the isolation and identification of <italic>Vibrio<\/italic> spp. Samples are then serially diluted in alkaline peptone water and streak on Thiosulfate Citrate Bile Salt Sucrose-TCBS agar for quantification of <italic>V<\/italic>. spp. For <italic>V. cholera<\/italic> isolation water samples were first enriched in nutrient broth at 37 \u00b0C for 16 hours followed by cultivation in selective media; TCBS agar at 37 \u00b0C for 24 hours. Yellow colonies on TCBS agar were screed as <italic>V. cholera<\/italic> and was confirmed by analyzing their biochemical characteristics like Catalase, Oxidase, MR, VP, Indole, Sugar fermentation. Following isolation antibiotic sensitivity test was performed on each <italic>V. cholera<\/italic> isolates to determine their antibiotic sensitivity profile. The results showed, out of 45 samples 12 contained <italic>V. cholera<\/italic>. Tube-well water has significantly lower concentration (log CFU\/mL) of <italic>V<\/italic>. spp. than river and pond water (P &lt; 0.05). Bacterial concentration doesn't deviate (P &gt; 0.05) significantly in 5 different location the sample was collected from. All the 12 isolates were sensitive to Gentamicin and ciprofloxacin (100%), while Chloramphenicol (91.67%), Sulfamethoxazole (91.67%), Azithromycin (66.67%) showed high sensitivity. Isolates showed marginal sensitivity towards Tetracycline (33.33%), and Cephalexin (16.67%) and 100% resistance against antibiotics like Vancomycin, Penicillin, Erythromycin, and Nalidixic Acid. Based on these data we recommend using tube-well water instead of river and pond water for drinking purposes. Furthermore, we suggest selective use of sensitive antimicrobials listed here for therapeutics of cholera outbreak.<\/p>\n<\/abstract><\/jats:p>","DOI":"10.3934\/microbiol.2021028","type":"journal-article","created":{"date-parts":[[2021,11,22]],"date-time":"2021-11-22T03:42:20Z","timestamp":1637552540000},"page":"471-480","source":"Crossref","is-referenced-by-count":5,"title":["Characterization of <i>Vibrio<\/i> spp. in environmental water samples collected from flood prone areas of Bangladesh and their antibiotic resistance profile"],"prefix":"10.3934","volume":"7","author":[{"given":"Md. Aoulad","family":"Hosen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Department of Microbiology, Gono Bishwabidyalay, Dhaka, Bangladesh","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fozol Korim","family":"Ovi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harunur","family":"Rashid","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MD. Hasibul","family":"Hasan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Md. Abdul","family":"Khalek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mahmudul","family":"Hasan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Farhana","family":"Easmin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nazmi Ara","family":"Rumi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad Shariful","family":"Islam","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Sylhet Agricultural University, Sylhet, Bangladesh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Department of Microbiology, Primeasia, Dhaka, Bangladesh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Department of Microbiology, Hajee Mohammad Danesh Science & Technology University Dinajpur, Bangladesh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Department of Microbiology, Jagannath University, Dhaka, Bangladesh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2321","reference":[{"key":"key-10.3934\/microbiol.2021028-1","doi-asserted-by":"crossref","unstructured":"Harris JB, LaRocque RC, Qadri F, et al. 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