{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T10:03:00Z","timestamp":1789466580987,"version":"build-2803163510"},"reference-count":63,"publisher":"American Institute of Mathematical Sciences (AIMS)","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["AIMSMICRO"],"published-print":{"date-parts":[[2024]]},"abstract":"<abstract><sec>\n<title>Background<\/title>\n<p>Extended-spectrum beta-lactamase (ESBL)-producing <italic>Enterobacteriaceae<\/italic> including <italic>Escherichia coli<\/italic> (<italic>E. coli<\/italic>), are recognized as a global public health threat due to their multidrug-resistant (MDR) phenotypes and their rapid dissemination in aquatic environments. Nevertheless, studies investigating the prevalence and antimicrobial resistance (AMR) profile of ESBL-producing <italic>E. coli<\/italic> in Lebanese surface water are limited.<\/p>\n<\/sec><sec>\n<title>Objective<\/title>\n<p>This study aimed to assess the physicochemical properties and microbial contamination load and to determine the distribution of AMR patterns of ESBL-producing <italic>E. coli<\/italic> in surface water samples from different sites in the North Governorate of Lebanon.<\/p>\n<\/sec><sec>\n<title>Methods<\/title>\n<p>Water samples were collected from 25 major sites in North Lebanon. These samples were analyzed for the presence of total coliforms, <italic>E. coli<\/italic>, and fecal enterococci. Phenotypic and genetic characterizations were then performed for <italic>E. coli<\/italic> isolates to determine their resistance patterns and phylogenetic groups.<\/p>\n<\/sec><sec>\n<title>Results<\/title>\n<p>Fifty-six samples out of 100 samples were positive for ESBL-producing <italic>E. coli<\/italic>, mostly harboring bla<sub>CTX-M<\/sub> (40\/56, 71%) including bla<sub>CTX-M-15<\/sub> (33\/40, 82%), bla<sub>TEM<\/sub> gene (36\/56, 64%), bla<sub>SHV<\/sub> (20\/56, 36%), and bla<sub>OXA<\/sub> (16\/56, 29%) including bla<sub>OXA-48<\/sub> gene (11\/16, 69%). Most ESBL-producing <italic>E. coli<\/italic> isolates belonged to the extra-intestinal pathogenic phylogroup B2 (40\/56, 71.4%) while 10\/56 (17.9%) belonged to the commensal phylogroup A.<\/p>\n<\/sec><sec>\n<title>Conclusion<\/title>\n<p>Our results highlight the need to implement effective water monitoring strategies to control transmission of ESBL-producing <italic>E. coli<\/italic> in surface water and thus reduce the burden on human and animal health.<\/p>\n<\/sec><\/abstract><\/jats:p>","DOI":"10.3934\/microbiol.2024018","type":"journal-article","created":{"date-parts":[[2024,5,10]],"date-time":"2024-05-10T02:35:56Z","timestamp":1715308556000},"page":"340-362","source":"Crossref","is-referenced-by-count":8,"title":["Phylogenetic group distribution and antibiotic resistance of <i>Escherichia coli<\/i> isolates in aquatic environments of a highly populated area"],"prefix":"10.3934","volume":"10","author":[{"given":"Rosette","family":"Mansour","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, Faculty of Sciences, Beirut Arab University, P.O. Box 11-5020, Riad El Solh 11072809, Beirut, Lebanon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad H.","family":"El-Dakdouki","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Sciences, Beirut Arab University, P.O. Box 11-5020, Riad El Solh 11072809, Beirut, Lebanon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Mina","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Sciences, Faculty of Health Sciences, Beirut Arab University, P.O. Box 11-5020, Riad El Solh 11072809, Beirut, Lebanon"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2321","reference":[{"key":"key-10.3934\/microbiol.2024018-1"},{"key":"key-10.3934\/microbiol.2024018-2"},{"key":"key-10.3934\/microbiol.2024018-3"},{"key":"key-10.3934\/microbiol.2024018-4"},{"key":"key-10.3934\/microbiol.2024018-5"},{"key":"key-10.3934\/microbiol.2024018-6"},{"key":"key-10.3934\/microbiol.2024018-7"},{"key":"key-10.3934\/microbiol.2024018-8"},{"key":"key-10.3934\/microbiol.2024018-9"},{"key":"key-10.3934\/microbiol.2024018-10"},{"key":"key-10.3934\/microbiol.2024018-11"},{"key":"key-10.3934\/microbiol.2024018-12"},{"key":"key-10.3934\/microbiol.2024018-13"},{"key":"key-10.3934\/microbiol.2024018-14"},{"key":"key-10.3934\/microbiol.2024018-15"},{"key":"key-10.3934\/microbiol.2024018-16"},{"key":"key-10.3934\/microbiol.2024018-17"},{"key":"key-10.3934\/microbiol.2024018-18"},{"key":"key-10.3934\/microbiol.2024018-19"},{"key":"key-10.3934\/microbiol.2024018-20"},{"key":"key-10.3934\/microbiol.2024018-21"},{"key":"key-10.3934\/microbiol.2024018-22"},{"key":"key-10.3934\/microbiol.2024018-23"},{"key":"key-10.3934\/microbiol.2024018-24"},{"key":"key-10.3934\/microbiol.2024018-25"},{"key":"key-10.3934\/microbiol.2024018-26"},{"key":"key-10.3934\/microbiol.2024018-27"},{"key":"key-10.3934\/microbiol.2024018-28"},{"key":"key-10.3934\/microbiol.2024018-29"},{"key":"key-10.3934\/microbiol.2024018-30"},{"key":"key-10.3934\/microbiol.2024018-31"},{"key":"key-10.3934\/microbiol.2024018-32"},{"key":"key-10.3934\/microbiol.2024018-33"},{"key":"key-10.3934\/microbiol.2024018-34"},{"key":"key-10.3934\/microbiol.2024018-35"},{"key":"key-10.3934\/microbiol.2024018-36"},{"key":"key-10.3934\/microbiol.2024018-37"},{"key":"key-10.3934\/microbiol.2024018-38"},{"key":"key-10.3934\/microbiol.2024018-39"},{"key":"key-10.3934\/microbiol.2024018-40"},{"key":"key-10.3934\/microbiol.2024018-41"},{"key":"key-10.3934\/microbiol.2024018-42"},{"key":"key-10.3934\/microbiol.2024018-43"},{"key":"key-10.3934\/microbiol.2024018-44"},{"key":"key-10.3934\/microbiol.2024018-45"},{"key":"key-10.3934\/microbiol.2024018-46"},{"key":"key-10.3934\/microbiol.2024018-47"},{"key":"key-10.3934\/microbiol.2024018-48"},{"key":"key-10.3934\/microbiol.2024018-49"},{"key":"key-10.3934\/microbiol.2024018-50"},{"key":"key-10.3934\/microbiol.2024018-51"},{"key":"key-10.3934\/microbiol.2024018-52"},{"key":"key-10.3934\/microbiol.2024018-53"},{"key":"key-10.3934\/microbiol.2024018-54"},{"key":"key-10.3934\/microbiol.2024018-55"},{"key":"key-10.3934\/microbiol.2024018-56"},{"key":"key-10.3934\/microbiol.2024018-57"},{"key":"key-10.3934\/microbiol.2024018-58"},{"key":"key-10.3934\/microbiol.2024018-59"},{"key":"key-10.3934\/microbiol.2024018-60"},{"key":"key-10.3934\/microbiol.2024018-61"},{"key":"key-10.3934\/microbiol.2024018-62"},{"key":"key-10.3934\/microbiol.2024018-63"}],"container-title":["AIMS Microbiology"],"original-title":[],"link":[{"URL":"http:\/\/www.aimspress.com\/article\/doi\/10.3934\/microbiol.2024018?viewType=html","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,10]],"date-time":"2024-05-10T02:38:41Z","timestamp":1715308721000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.aimspress.com\/article\/doi\/10.3934\/microbiol.2024018"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024]]},"references-count":63,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2024]]}},"URL":"https:\/\/doi.org\/10.3934\/microbiol.2024018","relation":{},"ISSN":["2471-1888"],"issn-type":[{"value":"2471-1888","type":"print"}],"subject":[],"published":{"date-parts":[[2024]]}}}