{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T22:27:59Z","timestamp":1774564079882,"version":"3.50.1"},"reference-count":32,"publisher":"American Institute of Mathematical Sciences (AIMS)","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["AIMSMICRO"],"published-print":{"date-parts":[[2022]]},"abstract":"<abstract>\n<p>In the present work, the microbiological quality of sesame, flaxseed, chia, pumpkin sunflower seeds, a mix of seeds, as well as flaxseed flour, marketed in southern Portugal, were studied through the counting of aerobic microorganisms at 30 \u00b0C (AM), molds and yeast (M&amp;Y), <italic>Escherichia coli<\/italic> (\u03b2-glucuronidase positive) (\u03b2-GP <italic>E. coli<\/italic>), <italic>Staphylococcus<\/italic> coagulase positive, and detection of <italic>Salmonella<\/italic> spp. The persistence of AM and M&amp;Y populations were also counted in organic and non-organic flaxseed at 20 \u00b0C for 11 months. The seeds with the highest average of AM were flaxseed (1.3 x 10<sup>6<\/sup> CFU\/g) followed by flaxseed flour (1.1 x 10<sup>6<\/sup> CFU\/g) while the lowest level was found in chia (2.9 x 10<sup>4<\/sup> CFU\/g). This seed also presented the lowest average values of filamentous fungi (9.8 x 10<sup>2<\/sup> CFU\/g), whereas sunflower seeds had the highest levels (1.7 x 10<sup>5<\/sup> CFU\/g). Flaxseed flour had the highest yeast counts (1.5 x 10<sup>4<\/sup> CFU\/g). Although some samples had high levels of AM and fungi, \u03b2-GP <italic>E. coli<\/italic> and <italic>Salmonella<\/italic> were not detected, therefore, they complied with the microbiological criteria of the European Union. The organic flaxseed contained higher numbers of AM and M&amp;Y than the non-organic ones (p &lt; 0.05). In addition, the storage of flaxseed at 20 \u00b0C resulted in changes of AM and M&amp;Y, showing that these populations were able to remain viable after eleven months (AM Log 5.4\u2013Log 5.6; M&amp;Y Log 2.8\u2013Log 4.1). The results obtained in the present study, namely those high levels of AM and fungi (&gt;10<sup>6<\/sup> and 10<sup>4<\/sup> CFU\/g respectively), alert to the need of improving processing practices, storage\/distribution conditions of edible seeds and derivatives, as well as the requirement of implementing adequate decontamination techniques.<\/p>\n<\/abstract><\/jats:p>","DOI":"10.3934\/microbiol.2022004","type":"journal-article","created":{"date-parts":[[2022,2,22]],"date-time":"2022-02-22T02:49:13Z","timestamp":1645498153000},"page":"42-52","source":"Crossref","is-referenced-by-count":8,"title":["Microbial quality of edible seeds commercially available in southern Portugal"],"prefix":"10.3934","volume":"8","author":[{"given":"Daniela","family":"Silva","sequence":"first","affiliation":[]},{"name":"Universidade do Algarve, Instituto Superior de Engenharia, Campus da Penha,8005-139, Faro Portugal","sequence":"first","affiliation":[]},{"given":"Patr\u00edcia","family":"Nunes","sequence":"additional","affiliation":[]},{"given":"Jessie","family":"Melo","sequence":"additional","affiliation":[]},{"given":"C\u00e9lia","family":"Quintas","sequence":"additional","affiliation":[]},{"name":"MED, Mediterranean Institute for Agriculture, Environment and Development, Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Portugal","sequence":"additional","affiliation":[]}],"member":"2321","reference":[{"key":"key-10.3934\/microbiol.2022004-1"},{"key":"key-10.3934\/microbiol.2022004-2"},{"key":"key-10.3934\/microbiol.2022004-3"},{"key":"key-10.3934\/microbiol.2022004-4"},{"key":"key-10.3934\/microbiol.2022004-5"},{"key":"key-10.3934\/microbiol.2022004-6"},{"key":"key-10.3934\/microbiol.2022004-7"},{"key":"key-10.3934\/microbiol.2022004-8"},{"key":"key-10.3934\/microbiol.2022004-9"},{"key":"key-10.3934\/microbiol.2022004-10"},{"key":"key-10.3934\/microbiol.2022004-11"},{"key":"key-10.3934\/microbiol.2022004-12"},{"key":"key-10.3934\/microbiol.2022004-13"},{"key":"key-10.3934\/microbiol.2022004-14"},{"key":"key-10.3934\/microbiol.2022004-15"},{"key":"key-10.3934\/microbiol.2022004-16"},{"key":"key-10.3934\/microbiol.2022004-17"},{"key":"key-10.3934\/microbiol.2022004-18"},{"key":"key-10.3934\/microbiol.2022004-19"},{"key":"key-10.3934\/microbiol.2022004-20"},{"key":"key-10.3934\/microbiol.2022004-21"},{"key":"key-10.3934\/microbiol.2022004-22"},{"key":"key-10.3934\/microbiol.2022004-23"},{"key":"key-10.3934\/microbiol.2022004-24"},{"key":"key-10.3934\/microbiol.2022004-25"},{"key":"key-10.3934\/microbiol.2022004-26"},{"key":"key-10.3934\/microbiol.2022004-27"},{"key":"key-10.3934\/microbiol.2022004-28"},{"key":"key-10.3934\/microbiol.2022004-29"},{"key":"key-10.3934\/microbiol.2022004-30"},{"key":"key-10.3934\/microbiol.2022004-31"},{"key":"key-10.3934\/microbiol.2022004-32"}],"container-title":["AIMS Microbiology"],"original-title":[],"link":[{"URL":"http:\/\/www.aimspress.com\/article\/doi\/10.3934\/microbiol.2022004?viewType=html","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,11]],"date-time":"2022-03-11T04:00:23Z","timestamp":1646971223000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.aimspress.com\/article\/doi\/10.3934\/microbiol.2022004"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022]]},"references-count":32,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2022]]}},"URL":"https:\/\/doi.org\/10.3934\/microbiol.2022004","relation":{},"ISSN":["2471-1888"],"issn-type":[{"value":"2471-1888","type":"print"}],"subject":[],"published":{"date-parts":[[2022]]}}}