{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T09:48:13Z","timestamp":1780480093313,"version":"3.54.1"},"reference-count":0,"publisher":"AVES YAYINCILIK A.\u015e.","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["AOTT"],"abstract":"<jats:p>Abstract\n\nObjectives: We investigated intra-articular levels of collagenase, which presumably promotes bone-tendon healing, and collagenase mediators involved in its production (tumor necrosis factor-alpha, TNF-?) and inhibition (TIMP-1 enzyme) in patients following anterior cruciate ligament (ACL) reconstruction. \\r\\nMethods: The study included 16 patients (15 males, 1 female; mean age 27 years; range 17 to 40 years) who underwent arthrocentesis due to effusion that developed following reconstruction of isolated ACL injuries. Intra-articular levels of collagenase, TNF-?, and TIMP-1 were measured using appropriate activity assay and immunoassay kits in synovial fluid samples obtained intraoperatively and during arthrocentesis. The mean time from ACL injury to surgical repair was 21\u00b110 months (range 1 to 72 months). Arthrocentesis was performed in a mean of 18 hours (range 12 to 36 hours) following ACL repair.\\r\\nResults: Measurements in synovial fluid samples obtained intra- and postoperatively yielded 1.49\u00b10.06 ng\/ml and 1.45\u00b10.05 ng\/ml for collagenase, 12\u00b15 ng\/ml and 22\u00b19.5 ng\/ml for TIMP-1, and 10.4\u00b17.1 pr\/ml and 14.11\u00b16.1 pr\/ml for TNF-?, respectively. Postoperative changes in the levels of collagenase (p=0.098) and TNF-? (p=0.069) were not significant, whereas increase in the TIMP-1 level was significant (p=0.026).\\r\\nConclusion: This study showed elevated levels of TNF-?, collagenase, and TIMP-1 due to the presence of ruptured ACL. Our findings showed how these levels changed in the acute postoperative period.\n\n\u00d6zet\n\nAma\u00e7: Bu \u00e7al\u0131\u015fmada, \u00f6n \u00e7apraz ba\u011f (\u00d6\u00c7B) cerrahisinden sonra kemik-tendon iyile\u015fmesinde etkili oldu\u011funu d\u00fc\u015f\u00fcnd\u00fc\u011f\u00fcm\u00fcz kollajenazlar\u0131n, bu kollajenazlar\u0131n sal\u0131n\u0131m\u0131n\u0131 d\u00fczenleyen sitokinlerden olan t\u00fcm\u00f6r nekroz fakt\u00f6r-alfa (TNF\u2013?) ve kollajenaz enzimlerinin do\u011fal inhibit\u00f6r\u00fc olan TIMP-1 enzimi d\u00fczeyleri sinovyal s\u0131v\u0131da ara\u015ft\u0131r\u0131ld\u0131.\\r\\n\u00c7al\u0131\u015fma plan\u0131: \u00c7al\u0131\u015fmaya, izole \u00d6\u00c7B cerrahisi uygulanan hastalar aras\u0131ndan, ameliyat sonras\u0131 takiplerde diz ekleminde ef\u00fczyon geli\u015fen ve bu nedenle ameliyat sonras\u0131 artrosentez yap\u0131lan 16 hasta (15 erkek, 1 kad\u0131n; ort. ya\u015f 27; da\u011f\u0131l\u0131m 17-40) al\u0131nd\u0131. Bu hastalardan ameliyat s\u0131ras\u0131nda ve sonras\u0131nda al\u0131nan sinovyal s\u0131v\u0131 \u00f6rneklerinde kollajenaz, TNF\u2013? ve TIMP-1 d\u00fczeyleri uygun kitler kullan\u0131larak \u00f6l\u00e7\u00fcld\u00fc. \u00d6n \u00e7apraz ba\u011f\u0131n kopmas\u0131 ile ameliyat aras\u0131nda ge\u00e7en s\u00fcre ortalama 21\u00b110 ay (da\u011f\u0131l\u0131m 1-72 ay), ameliyattan sonra artrosentez s\u0131ras\u0131nda eklemi\u00e7i s\u0131v\u0131 alma i\u00e7in ge\u00e7en s\u00fcre ortalama 18 saat (da\u011f\u0131l\u0131m 12-36 saat) idi.\\r\\nSonu\u00e7lar: Ameliyat s\u0131ras\u0131nda ve sonras\u0131nda al\u0131nan eklemi\u00e7i sinovyal s\u0131v\u0131 \u00f6rneklerinde \u00f6l\u00e7\u00fclen de\u011ferler kollajenaz i\u00e7in s\u0131ras\u0131yla 1.49\u00b10.06 ng\/ml ve 1.45\u00b10.05 ng\/ml, TIMP-1 i\u00e7in 12\u00b15 ng\/ml ve 22\u00b19.5 ng\/ml, TNF-? i\u00e7in 10.4\u00b17.1 pr\/ml ve 14.11\u00b16.1 pr\/ml bulundu. Kollajenaz ve TNF-? a\u00e7\u0131s\u0131ndan ameliyat sonras\u0131ndaki de\u011fi\u015fimlerde anlaml\u0131 farkl\u0131l\u0131k g\u00f6r\u00fclmedi (s\u0131ras\u0131yla, p=0.098 ve p=0.069); TIMP-1 d\u00fczeyindeki art\u0131\u015f ise anlaml\u0131yd\u0131 (p=0.026).\\r\\n\u00c7\u0131kar\u0131mlar: \u00c7al\u0131\u015fmam\u0131zdaki dizlerde \u00d6\u00c7B kopuk oldu\u011fundan, TNF-?, kollajenaz, TIMP-1 d\u00fczeyleri y\u00fcksek bulunmu\u015ftur. Verilerimiz bu de\u011ferlerin ameliyat sonras\u0131 yak\u0131n d\u00f6nemde nas\u0131l de\u011fi\u015fim ge\u00e7irdi\u011fini g\u00f6stermi\u015ftir.<\/jats:p>","DOI":"10.3944\/aott.2009.214","type":"journal-article","created":{"date-parts":[[2009,8,16]],"date-time":"2009-08-16T08:10:56Z","timestamp":1250410256000},"page":"214-218","source":"Crossref","is-referenced-by-count":14,"title":["Evaluation of intraarticular collagenase TIMP1 and TNF? levels before and after anterior cruciate ligament reconstruction"],"prefix":"10.5152","volume":"43","author":[{"given":"Burak","family":"Akesen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Burak","family":"Demirag","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ferah","family":"Budak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"3111","published-online":{"date-parts":[[2020,2,8]]},"container-title":["Acta Orthopaedica et Traumatologica Turcica"],"original-title":[],"link":[{"URL":"https:\/\/aott.org.tr\/index.php\/pub\/article\/download\/2268\/2225","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T11:00:17Z","timestamp":1763463617000},"score":1,"resource":{"primary":{"URL":"https:\/\/aott.org.tr\/index.php\/pub\/article\/view\/2268"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,2,8]]},"references-count":0,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2009,1,3]]}},"URL":"https:\/\/doi.org\/10.3944\/aott.2009.214","relation":{},"ISSN":["2589-1294","1017-995X"],"issn-type":[{"value":"2589-1294","type":"electronic"},{"value":"1017-995X","type":"print"}],"subject":[],"published":{"date-parts":[[2020,2,8]]}}}