{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,3]],"date-time":"2026-03-03T23:19:53Z","timestamp":1772579993024,"version":"3.50.1"},"reference-count":55,"publisher":"Japanese Society for Dental Materials and Devices","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Dent. Mater. J.","Dental Materials Journal"],"published-print":{"date-parts":[[2021,9,25]]},"DOI":"10.4012\/dmj.2020-404","type":"journal-article","created":{"date-parts":[[2021,5,20]],"date-time":"2021-05-20T22:33:37Z","timestamp":1621550017000},"page":"1160-1168","source":"Crossref","is-referenced-by-count":12,"title":["Dentin adhesives application deviations: Effects on permeability and nanoleakage"],"prefix":"10.4012","volume":"40","author":[{"given":"Alexandre","family":"CAVALHEIRO","sequence":"first","affiliation":[{"name":"Department of Operative Dentistry, Faculty of Dental Medicine, Universidade de Lisboa, Rua Professora Teresa Ambr\u00f3sio, Cidade Universit\u00e1ria"}]},{"given":"Joana","family":"CRUZ","sequence":"additional","affiliation":[{"name":"Department of Operative Dentistry, Faculty of Dental Medicine, Universidade de Lisboa, Rua Professora Teresa Ambr\u00f3sio, Cidade Universit\u00e1ria"}]},{"given":"Bernardo","family":"SOUSA","sequence":"additional","affiliation":[{"name":"Department of Operative Dentistry, Faculty of Dental Medicine, Universidade de Lisboa, Rua Professora Teresa Ambr\u00f3sio, Cidade Universit\u00e1ria"}]},{"given":"Ana","family":"SILVA","sequence":"additional","affiliation":[{"name":"Department of Operative Dentistry, Faculty of Dental Medicine, Universidade de Lisboa, Rua Professora Teresa Ambr\u00f3sio, Cidade Universit\u00e1ria"}]},{"given":"Catarina","family":"COITO","sequence":"additional","affiliation":[{"name":"Department of Operative Dentistry, Faculty of Dental Medicine, Universidade de Lisboa, Rua Professora Teresa Ambr\u00f3sio, Cidade Universit\u00e1ria"}]},{"given":"Manuela","family":"LOPES","sequence":"additional","affiliation":[{"name":"Department of Cellular and Molecular Biology, Faculty of Dental Medicine, Universidade de Lisboa, Rua Professora Teresa Ambr\u00f3sio, Cidade Universit\u00e1ria"}]},{"given":"Marcos","family":"VARGAS","sequence":"additional","affiliation":[{"name":"Department of Family Dentistry, College of Dentistry, University of Iowa"}]}],"member":"2427","reference":[{"key":"1","doi-asserted-by":"crossref","unstructured":"1) Cadenaro M, Maravic T, Comba A, Mazzoni A, Fanfoni L, Hilton T, <i>et al.<\/i> The role of polymerization in adhesive dentistry. Dent Mater 2019; 35: e1-e22.","DOI":"10.1016\/j.dental.2018.11.012"},{"key":"2","doi-asserted-by":"crossref","unstructured":"2) Besnault C, Attal JP. Influence of a simulated oral environment on microleakage of two adhesive systems in Class II composite restorations. J Dent 2002; 30: 1-6.","DOI":"10.1016\/S0300-5712(01)00050-1"},{"key":"3","doi-asserted-by":"crossref","unstructured":"3) Reis AF, Giannini M, Pereira PN. Long-term TEM analysis of the nanoleakage patterns in resin-dentin interfaces produced by different bonding strategies. Dent Mater 2007; 23: 1164-1172.","DOI":"10.1016\/j.dental.2006.10.006"},{"key":"4","doi-asserted-by":"crossref","unstructured":"4) Hashimoto M, Tay FR, Svizero NR, de Gee AJ, Feilzer AJ, Sano H, <i>et al.<\/i> The effects of common errors on sealing ability of total-etch adhesives. Dent Mater 2006; 22: 560-568.","DOI":"10.1016\/j.dental.2005.06.004"},{"key":"5","unstructured":"5) Tay FR, Pashley DH, Yiu C, Cheong C, Hashimoto M, Itou K, <i>et al.<\/i> Nanoleakage types and potential implications: evidence from unfilled and filled adhesives with the same resin composition. Am J Dent 2004; 17: 182-190."},{"key":"6","unstructured":"6) Cavalheiro A, Vargas MA, Armstrong SR, Dawson DV, Gratton DG. Effect of incorrect primer application on dentin permeability. J Adhes Dent 2006; 8: 393-400."},{"key":"7","unstructured":"7) Scotti N, Cavalli G, Gagliani M, Breschi L. New adhesives and bonding techniques. Why and when? Int J Esthet Dent 2017; 12: 524-535."},{"key":"8","unstructured":"8) Cruz J, Sousa B, Coito C, Lopes M, Vargas M, Cavalheiro A. Microtensile bond strength to dentin and enamel of self-etch vs. etch-and-rinse modes of universal adhesives. Am J Dent 2019; 32: 174-182."},{"key":"9","unstructured":"9) Frankenberger R, Kramer N, Petschelt A. Technique sensitivity of dentin bonding: effect of application mistakes on bond strength and marginal adaptation. Oper Dent 2000; 25: 324-330."},{"key":"10","unstructured":"10) Peschke A, Blunck U, Roulet JF. Influence of incorrect application of a water-based adhesive system on the marginal adaptation of Class V restorations. Am J Dent 2000; 13: 239-244."},{"key":"11","unstructured":"11) Peutzfeldt A, Asmussen E. Adhesive systems: effect on bond strength of incorrect use. J Adhes Dent 2002; 4: 233-242."},{"key":"12","doi-asserted-by":"crossref","unstructured":"12) Armstrong SR, Keller JC, Boyer DB. The influence of water storage and C-factor on the dentin-resin composite microtensile bond strength and debond pathway utilizing a filled and unfilled adhesive resin. Dent Mater 2001; 17: 268-276.","DOI":"10.1016\/S0109-5641(00)00081-6"},{"key":"13","doi-asserted-by":"crossref","unstructured":"13) Sahin C, Cehreli ZC, Yenigul M, Dayangac B. In vitro permeability of etch-and-rinse and self-etch adhesives used for immediate dentin sealing. Dent Mater J 2012; 31: 401-408.","DOI":"10.4012\/dmj.2011-217"},{"key":"14","unstructured":"14) Sano H, Yoshiyama M, Ebisu S, Burrow MF, Takatsu T, Ciucchi B, <i>et al.<\/i> Comparative SEM and TEM observations of nanoleakage within the hybrid layer. Oper Dent 1995; 20: 160-167."},{"key":"15","unstructured":"15) Li H, Burrow MF, Tyas MJ. Nanoleakage of cervical restorations of four dentin bonding systems. J Adhes Dent 2000; 2: 57-65."},{"key":"16","doi-asserted-by":"crossref","unstructured":"16) Tao L, Pashley DH. The relationship between dentin bond strengths and dentin permeability. Dent Mater 1989; 5: 133-139.","DOI":"10.1016\/0109-5641(89)90144-9"},{"key":"17","doi-asserted-by":"crossref","unstructured":"17) Br\u00e4nnstr\u00f6m M. Sensitivity of dentine. Oral Surg Oral Med Oral Pathol 1966; 21: 517-526.","DOI":"10.1016\/0030-4220(66)90411-7"},{"key":"18","unstructured":"18) Br\u00e4nnstr\u00f6m M. Etiology of dentin hypersensitivity. Proc Finn Dent Soc 1992; 88: 7-13."},{"key":"19","unstructured":"19) Pashley DH, Carvalho RM, Pereira JC, Villanueva R, Tay FR. The use of oxalate to reduce dentin permeability under adhesive restorations. Am J Dent 2001; 14: 89-94."},{"key":"20","unstructured":"20) Tay FR, Pashley DH. Have dentin adhesives become too hydrophilic? J Can Dent Assoc 2003; 69: 726-731."},{"key":"21","doi-asserted-by":"crossref","unstructured":"21) Tay FR, Pashley DH, Mak YF, Carvalho RM, Lai SC, Suh BI. Integrating oxalate desensitizers with total-etch two-step adhesive. J Dent Res 2003; 82: 703-707.","DOI":"10.1177\/154405910308200909"},{"key":"22","unstructured":"22) Dental materials \u2014Testing of adhesion to tooth structure. International Organization for Standardization. Technical report TR 11405, 1-14. 1994, Switzerland."},{"key":"23","unstructured":"23) Kolker JL, Vargas MA, Armstrong SR, Dawson DV. Effect of desensitizing agents on dentin permeability and dentin tubule occlusion. J Adhes Dent 2002; 4: 211-221."},{"key":"24","doi-asserted-by":"crossref","unstructured":"24) Yiu CK, Hiraishi N, Chersoni S, Breschi L, Ferrari M, Prati C, <i>et al.<\/i> Single-bottle adhesives behave as permeable membranes after polymerisation. II. Differential permeability reduction with an oxalate desensitiser. J Dent 2006; 34: 106-116.","DOI":"10.1016\/j.jdent.2005.05.001"},{"key":"25","doi-asserted-by":"crossref","unstructured":"25) Pashley DH, Tao L, Boyd L, King GE, Horner JA. Scanning electron microscopy of the substructure of smear layers in human dentine. Arch Oral Biol 1988; 33: 265-270.","DOI":"10.1016\/0003-9969(88)90188-4"},{"key":"26","doi-asserted-by":"crossref","unstructured":"26) Outhwaite WC, McKenzie DM, Pashley DH. A versatile split-chamber device for studying dentin permeability. J Dent Res 1974; 53: 1503.","DOI":"10.1177\/00220345740530064101"},{"key":"27","unstructured":"27) Pashley D. Dentin Permeability: Theory and Practice. In: Spangberg LSW, editor. Experimental Endodontics. Boca Raton, FL: CRC Press; 1990. p. 20-46."},{"key":"28","doi-asserted-by":"crossref","unstructured":"28) Ozok AR, Wu MK, Wesselink PR. The effects of post-extraction time on the hydraulic conductance of human dentine in vitro. Arch Oral Biol 2002; 47: 41-46.","DOI":"10.1016\/S0003-9969(01)00090-5"},{"key":"29","doi-asserted-by":"crossref","unstructured":"29) Goodis HE, Marshall GW Jr, White JM, Gee L, Hornberger B, Marshall SJ. Storage effects on dentin permeability and shear bond strengths. Dent Mater 1993; 9: 79-84.","DOI":"10.1016\/0109-5641(93)90079-6"},{"key":"30","doi-asserted-by":"crossref","unstructured":"30) Faul F, Erdfelder E, Buchner A, Lang AG. Statistical power analyses using G*Power 3.1: tests for correlation and regression analyses. Behav Res Methods 2009; 41: 1149-1160.","DOI":"10.3758\/BRM.41.4.1149"},{"key":"31","doi-asserted-by":"crossref","unstructured":"31) Faul F, Erdfelder E, Lang AG, Buchner A. G*Power 3: a flexible statistical power analysis program for the social, behavioral, and biomedical sciences. Behav Res Methods 2007; 39: 175-191.","DOI":"10.3758\/BF03193146"},{"key":"32","unstructured":"32) Conover WJ. Practical Nonparametric Statistics. 3rd ed. New York: Wiley; 1999."},{"key":"33","unstructured":"33) Levene H. Essays in Honor of Harold Hoteling, I. In: Olkin, editor. Contributions to Probability and Statistics: Stanford University Press; 1960. p. 278-292."},{"key":"34","doi-asserted-by":"crossref","unstructured":"34) Tay FR, Pashley DH, Yoshiyama M. Two modes of nanoleakage expression in single-step adhesives. J Dent Res 2002; 81: 472-476.","DOI":"10.1177\/154405910208100708"},{"key":"35","doi-asserted-by":"crossref","unstructured":"35) Prati C. What is the clinical relevance of in vitro dentine permeability tests? J Dent 1994; 22: 83-88.","DOI":"10.1016\/0300-5712(94)90005-1"},{"key":"36","doi-asserted-by":"crossref","unstructured":"36) Ciucchi B, Bouillaguet S, Holz J, Pashley D. Dentinal fluid dynamics in human teeth, in vivo. J Endod 1995; 21: 191-194.","DOI":"10.1016\/S0099-2399(06)80564-9"},{"key":"37","doi-asserted-by":"crossref","unstructured":"37) Luque-Martinez IV, Perdigao J, Munoz MA, Sezinando A, Reis A, Loguercio AD. Effects of solvent evaporation time on immediate adhesive properties of universal adhesives to dentin. Dent Mater 2014; 30: 1126-1135.","DOI":"10.1016\/j.dental.2014.07.002"},{"key":"38","doi-asserted-by":"crossref","unstructured":"38) Irmak O, Baltacioglu IH, Ulusoy N, Bagis YH. Solvent type influences bond strength to air or blot-dried dentin. BMC Oral Health 2016; 16: 77.","DOI":"10.1186\/s12903-016-0247-3"},{"key":"39","unstructured":"39) Itthagarun A, Tay FR, Pashley DH, Wefel JS, Garcia-Godoy F, Wei SH. Single-step, self-etch adhesives behave as permeable membranes after polymerization. Part III. Evidence from fluid conductance and artificial caries inhibition. Am J Dent 2004; 17: 394-400."},{"key":"40","doi-asserted-by":"crossref","unstructured":"40) Bouillaguet S, Bertossa B, Krejci I, Wataha JC, Tay FR, Pashley DH. Alternative adhesive strategies to optimize bonding to radicular dentin. J Endod 2007; 33: 1227-1230.","DOI":"10.1016\/j.joen.2007.05.016"},{"key":"41","unstructured":"41) King NM, Tay FR, Pashley DH, Hashimoto M, Ito S, Brackett WW, <i>et al.<\/i> Conversion of one-step to two-step self-etch adhesives for improved efficacy and extended application. Am J Dent 2005; 18: 126-134."},{"key":"42","unstructured":"42) Barkmeier WW, Erickson RL. Shear bond strength of composite to enamel and dentin using Scotchbond Multi-Purpose. Am J Dent 1994; 7: 175-179."},{"key":"43","doi-asserted-by":"crossref","unstructured":"43) Hiraishi N, Breschi L, Prati C, Ferrari M, Tagami J, King NM. Technique sensitivity associated with air-drying of HEMA-free, single-bottle, one-step self-etch adhesives. Dent Mater 2007; 23: 498-505.","DOI":"10.1016\/j.dental.2006.03.007"},{"key":"44","doi-asserted-by":"crossref","unstructured":"44) Giannini M, Makishi P, Ayres AP, Vermelho PM, Fronza BM, Nikaido T, <i>et al.<\/i> Self-etch adhesive systems: a literature review. Braz Dent J 2015; 26: 3-10.","DOI":"10.1590\/0103-6440201302442"},{"key":"45","doi-asserted-by":"crossref","unstructured":"45) Saikaew P, Matsumoto M, Chowdhury A, Carvalho RM, Sano H. Does shortened application time affect long-term bond strength of universal adhesives to dentin? Oper Dent 2018; 43: 549-558.","DOI":"10.2341\/17-205-L"},{"key":"46","doi-asserted-by":"crossref","unstructured":"46) Mena-Serrano AP, Garcia EJ, Perez MM, Martins GC, Grande RH, Loguercio AD, <i>et al.<\/i> Effect of the application time of phosphoric acid and self-etch adhesive systems to sclerotic dentin. J Appl Oral Sci 2013; 21: 196-202.","DOI":"10.1590\/1678-7757201302136"},{"key":"47","doi-asserted-by":"crossref","unstructured":"47) Sadek FT, Pashley DH, Ferrari M, Tay FR. Tubular occlusion optimizes bonding of hydrophobic resins to dentin. J Dent Res 2007; 86: 524-528.","DOI":"10.1177\/154405910708600607"},{"key":"48","doi-asserted-by":"crossref","unstructured":"48) Yiu CK, King NM, Suh BI, Sharp LJ, Carvalho RM, Pashley DH, <i>et al.<\/i> Incompatibility of oxalate desensitizers with acidic, fluoride-containing total-etch adhesives. J Dent Res 2005; 84: 730-735.","DOI":"10.1177\/154405910508400809"},{"key":"49","doi-asserted-by":"crossref","unstructured":"49) Gillam DG, Mordan NJ, Sinodinou AD, Tang JY, Knowles JC, Gibson IR. The effects of oxalate-containing products on the exposed dentine surface: an SEM investigation. J Oral Rehabil 2001; 28: 1037-1044.","DOI":"10.1046\/j.1365-2842.2001.00775.x"},{"key":"50","unstructured":"50) Hashimoto M, Sano H, Yoshida E, Hori M, Kaga M, Oguchi H, <i>et al.<\/i> Effects of multiple adhesive coatings on dentin bonding. Oper Dent 2004; 29: 416-423."},{"key":"51","doi-asserted-by":"crossref","unstructured":"51) Fujiwara S, Takamizawa T, Barkmeier WW, Tsujimoto A, Imai A, Watanabe H, <i>et al.<\/i> Effect of double-layer application on bond quality of adhesive systems. J Mech Behav Biomed Mater 2018; 77: 501-509.","DOI":"10.1016\/j.jmbbm.2017.10.008"},{"key":"52","unstructured":"52) Momoi Y, Akimoto N, Kida K, Yip KH, Kohno A. Sealing ability of dentin coating using adhesive resin systems. Am J Dent 2003; 16: 105-111."},{"key":"53","doi-asserted-by":"crossref","unstructured":"53) Gregoire G, Joniot S, Guignes P, Millas A. Dentin permeability: self-etching and one-bottle dentin bonding systems. J Prosthet Dent 2003; 90: 42-49.","DOI":"10.1016\/S0022-3913(03)00258-0"},{"key":"54","doi-asserted-by":"crossref","unstructured":"54) Tay FR, Hashimoto M, Pashley DH, Peters MC, Lai SC, Yiu CK, <i>et al.<\/i> Aging affects two modes of nanoleakage expression in bonded dentin. J Dent Res 2003; 82: 537-541.","DOI":"10.1177\/154405910308200710"},{"key":"55","doi-asserted-by":"crossref","unstructured":"55) Hashimoto M, Ohno H, Sano H, Tay FR, Kaga M, Kudou Y, <i>et al.<\/i> Micromorphological changes in resin-dentin bonds after 1 year of water storage. J Biomed Mater Res 2002; 63: 306-311.","DOI":"10.1002\/jbm.10208"}],"container-title":["Dental Materials Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jstage.jst.go.jp\/article\/dmj\/40\/5\/40_2020-404\/_pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,10,2]],"date-time":"2021-10-02T06:38:17Z","timestamp":1633156697000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jstage.jst.go.jp\/article\/dmj\/40\/5\/40_2020-404\/_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,9,25]]},"references-count":55,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2021]]}},"URL":"https:\/\/doi.org\/10.4012\/dmj.2020-404","relation":{},"ISSN":["0287-4547","1881-1361"],"issn-type":[{"value":"0287-4547","type":"print"},{"value":"1881-1361","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,9,25]]},"article-number":"2020-404"}}