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This protein showed sequence similarity to a well-known T cell adaptor protein, adhesion and degranulation-promoting adaptor protein (ADAP); therefore, the novel protein was designated activation-dependent, raft-recruited ADAP-like phosphoprotein (ARAP). Suppression of ARAP impaired the major signaling pathways downstream of the TCR. ARAP associated with the Src homology 2 domain of Src homology 2\u2013containing leukocyte protein of 76 kDa via the phosphorylation of two YDDV motifs in response to TCR stimulation. ARAP also mediated integrin activation but was not involved in actin polymerization. The results of this study indicate that a novel T cell adaptor protein, ARAP, plays a unique role in T cells as a part of both the proximal activation signaling and inside\u2013out signaling pathways that result in integrin activation and T cell adhesion.<\/jats:p>","DOI":"10.4049\/jimmunol.1501913","type":"journal-article","created":{"date-parts":[[2016,6,23]],"date-time":"2016-06-23T14:16:16Z","timestamp":1466691376000},"page":"942-952","source":"Crossref","is-referenced-by-count":16,"title":["ARAP, a Novel Adaptor Protein, Is Required for TCR Signaling and Integrin-Mediated Adhesion"],"prefix":"10.1093","volume":"197","author":[{"given":"Seung Hee","family":"Jung","sequence":"first","affiliation":[{"name":"Department of Life Science, College of Natural Sciences, Ewha Womans University , Seoul 03760,","place":["Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eun Hye","family":"Yoo","sequence":"additional","affiliation":[{"name":"Department of Life Science, College of Natural Sciences, Ewha Womans University , Seoul 03760,","place":["Korea"]},{"name":"Research Center for Cellular Homeostasis, Ewha Womans University , Seoul 03760,","place":["Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mi Jin","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Life Science, College of Natural Sciences, Ewha Womans University , Seoul 03760,","place":["Korea"]},{"name":"Research Center for Cellular Homeostasis, Ewha Womans University , Seoul 03760,","place":["Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyeon Myeong","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Life Science, College of Natural Sciences, Ewha Womans University , Seoul 03760,","place":["Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hee Yoon","family":"Kang","sequence":"additional","affiliation":[{"name":"Department of Life Science, College of Natural Sciences, Ewha Womans University , Seoul 03760,","place":["Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Je-Yoel","family":"Cho","sequence":"additional","affiliation":[{"name":"Department of Veterinary Biochemistry, College of Veterinary Medicine, Seoul National University , Seoul 08826,","place":["Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jong Ran","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Life Science, College of Natural Sciences, Ewha Womans University , Seoul 03760,","place":["Korea"]},{"name":"Research Center for Cellular Homeostasis, Ewha Womans University , Seoul 03760,","place":["Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2016,8,1]]},"reference":[{"key":"2025030617465409700_r1","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1126\/science.285.5425.221","article-title":"The immunological synapse: a molecular machine controlling T cell activation","volume":"285","author":"Grakoui","year":"1999","journal-title":"Science"},{"key":"2025030617465409700_r2","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1146\/annurev.immunol.19.1.375","article-title":"The immunological synapse","volume":"19","author":"Bromley","year":"2001","journal-title":"Annu. 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