{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T07:20:35Z","timestamp":1778224835269,"version":"3.51.4"},"reference-count":30,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2025,1,6]],"date-time":"2025-01-06T00:00:00Z","timestamp":1736121600000},"content-version":"vor","delay-in-days":2715,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["immunohorizons.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2017,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Tissue infiltration by circulating monocytes is a critical step in the initiation and augmentation of type 2 inflammatory responses in the lungs. Our studies demonstrate that IL-33\u2212\/\u2212 mice have a defect in monocyte extravasation from the vasculature to the lung interstitium during induction of type 2 inflammatory responses. This result suggests that monocyte migration to the lungs is IL-33 dependent, and we found that administration of exogenous recombinant IL-33 is sufficient to restore monocyte localization to the lung interstitium. Further investigation of the effect of early administration of recombinant IL-33 on the lungs identified upregulation of multiple chemokines including the monocyte chemoattractants CCL2, CCL7, and CCL22. Importantly, blockade of G-protein coupled receptor\u2013dependent signaling, and thereby chemokine receptor activity, inhibited IL-33\u2013driven monocyte recruitment. CCR2 deficiency prevented recruitment of monocytes to the lung extravascular space during allergic sensitization, and resulted in reduced eosinophilia after allergen challenge. Thus, IL-33 plays a critical role in the initiation of type 2 inflammatory responses by inducing upregulation of chemokines that promote monocyte recruitment to the lung interstitium.<\/jats:p>","DOI":"10.4049\/immunohorizons.1700024","type":"journal-article","created":{"date-parts":[[2017,8,16]],"date-time":"2017-08-16T00:30:21Z","timestamp":1502843421000},"page":"101-108","update-policy":"https:\/\/doi.org\/10.4049\/immunohorizon.f110.005975","source":"Crossref","is-referenced-by-count":12,"title":["IL-33 Drives Monocyte Recruitment to Lung Interstitium through Chemokine Upregulation"],"prefix":"10.1093","volume":"1","author":[{"given":"Melissa Y","family":"Tjota","sequence":"first","affiliation":[{"name":"Committee on Immunology, University of Chicago , Chicago, IL 60637"},{"name":"Medical Scientist Training Program, University of Chicago , Chicago, IL 60637"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1605-878X","authenticated-orcid":false,"given":"Daniel F","family":"Camacho","sequence":"additional","affiliation":[{"name":"Committee on Immunology, University of Chicago , Chicago, IL 60637"},{"name":"Medical Scientist Training Program, University of Chicago , Chicago, IL 60637"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heth R","family":"Turnquist","sequence":"additional","affiliation":[{"name":"Department of Surgery, Thomas E. Starzl Transplantation Institute, University of Pittsburgh School of Medicine , Pittsburgh, PA 15261"},{"name":"Department of Immunology, University of Pittsburgh School of Medicine , Pittsburgh, PA 15213"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne I","family":"Sperling","sequence":"additional","affiliation":[{"name":"Committee on Immunology, University of Chicago , Chicago, IL 60637"},{"name":"Department of Medicine, Section of Pulmonary and Critical Care Medicine, University of Chicago , Chicago, IL 60637"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2017,8,15]]},"reference":[{"key":"2025010608402852800_r1","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1016\/j.cell.2010.03.004","article-title":"Asthma and allergic inflammation","volume":"140","author":"Locksley","year":"2010","journal-title":"Cell"},{"key":"2025010608402852800_r2","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1038\/ni.3049","article-title":"The immunology of asthma","volume":"16","author":"Lambrecht","year":"2015","journal-title":"Nat. Immunol."},{"key":"2025010608402852800_r3","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1016\/j.iac.2016.03.006","article-title":"Eosinophilic endotype of asthma","volume":"36","author":"Aleman","year":"2016","journal-title":"Immunol. Allergy Clin. North Am."},{"key":"2025010608402852800_r4","doi-asserted-by":"crossref","first-page":"4245","DOI":"10.4049\/jimmunol.1400580","article-title":"Resident alveolar macrophages suppress, whereas recruited monocytes promote, allergic lung inflammation in murine models of asthma","volume":"193","author":"Zas\u0142ona","year":"2014","journal-title":"J. Immunol."},{"key":"2025010608402852800_r5","doi-asserted-by":"crossref","first-page":"e0157571","DOI":"10.1371\/journal.pone.0157571","article-title":"Interleukin-33 from monocytes recruited to the lung contributes to house dust mite-induced airway inflammation in a mouse model","volume":"11","author":"Tashiro","year":"2016","journal-title":"PLoS One"},{"key":"2025010608402852800_r6","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1038\/nri.2017.28","article-title":"Monocyte differentiation and antigen-presenting functions","volume":"17","author":"Jakubzick","year":"2017","journal-title":"Nat. Rev. Immunol."},{"key":"2025010608402852800_r7","doi-asserted-by":"crossref","first-page":"669","DOI":"10.1146\/annurev.immunol.021908.132557","article-title":"Blood monocytes: development, heterogeneity, and relationship with dendritic cells","volume":"27","author":"Auffray","year":"2009","journal-title":"Annu. Rev. Immunol."},{"key":"2025010608402852800_r8","doi-asserted-by":"crossref","first-page":"416","DOI":"10.1161\/CIRCRESAHA.111.253377","article-title":"NR4A1 (Nur77) deletion polarizes macrophages toward an inflammatory phenotype and increases atherosclerosis","volume":"110","author":"Hanna","year":"2012","journal-title":"Circ. Res."},{"key":"2025010608402852800_r9","doi-asserted-by":"crossref","first-page":"392","DOI":"10.1038\/nri3671","article-title":"Monocytes and macrophages: developmental pathways and tissue homeostasis","volume":"14","author":"Ginhoux","year":"2014","journal-title":"Nat. Rev. Immunol."},{"key":"2025010608402852800_r10","doi-asserted-by":"crossref","first-page":"4410","DOI":"10.4049\/jimmunol.172.7.4410","article-title":"Subpopulations of mouse blood monocytes differ in maturation stage and inflammatory response","volume":"172","author":"Sunderk\u00f6tter","year":"2004","journal-title":"J. Immunol."},{"key":"2025010608402852800_r11","doi-asserted-by":"crossref","first-page":"762","DOI":"10.1038\/nri3070","article-title":"Monocyte recruitment during infection and inflammation","volume":"11","author":"Shi","year":"2011","journal-title":"Nat. Rev. Immunol."},{"key":"2025010608402852800_r12","doi-asserted-by":"crossref","first-page":"666","DOI":"10.1126\/science.1142883","article-title":"Monitoring of blood vessels and tissues by a population of monocytes with patrolling behavior","volume":"317","author":"Auffray","year":"2007","journal-title":"Science"},{"key":"2025010608402852800_r13","doi-asserted-by":"crossref","first-page":"e0144133","DOI":"10.1371\/journal.pone.0144133","article-title":"CD40-TRAF signaling upregulates CX3CL1 and TNF-\u03b1 in human aortic endothelial cells but not in retinal endothelial cells","volume":"10","author":"Greene","year":"2015","journal-title":"PLoS One"},{"key":"2025010608402852800_r14","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1146\/annurev.immunol.26.021607.090326","article-title":"Monocyte-mediated defense against microbial pathogens","volume":"26","author":"Serbina","year":"2008","journal-title":"Annu. Rev. Immunol."},{"key":"2025010608402852800_r15","doi-asserted-by":"crossref","first-page":"902","DOI":"10.1172\/JCI29919","article-title":"Critical roles for CCR2 and MCP-3 in monocyte mobilization from bone marrow and recruitment to inflammatory sites","volume":"117","author":"Tsou","year":"2007","journal-title":"J. Clin. Invest."},{"key":"2025010608402852800_r16","doi-asserted-by":"crossref","first-page":"2545","DOI":"10.4049\/jimmunol.1200689","article-title":"Chitin elicits CCL2 from airway epithelial cells and induces CCR2-dependent innate allergic inflammation in the lung","volume":"189","author":"Roy","year":"2012","journal-title":"J. Immunol."},{"key":"2025010608402852800_r17","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1111\/j.1365-2222.2009.03384.x","article-title":"IL-33: a tissue derived cytokine pathway involved in allergic inflammation and asthma","volume":"40","author":"Smith","year":"2010","journal-title":"Clin. Exp. Allergy"},{"key":"2025010608402852800_r18","doi-asserted-by":"crossref","first-page":"706","DOI":"10.1016\/j.jaci.2014.06.013","article-title":"Signaling through FcR\u03b3-associated receptors on dendritic cells drives IL-33-dependent TH2-type responses","volume":"134","author":"Tjota","year":"2014","journal-title":"J. Allergy Clin. Immunol."},{"key":"2025010608402852800_r19","doi-asserted-by":"crossref","first-page":"2287","DOI":"10.1172\/JCI63802","article-title":"IL-33-dependent induction of allergic lung inflammation by Fc\u03b3RIII signaling","volume":"123","author":"Tjota","year":"2013","journal-title":"J. Clin. Invest."},{"key":"2025010608402852800_r20","doi-asserted-by":"crossref","first-page":"18581","DOI":"10.1073\/pnas.1003059107","article-title":"IL-33 is a crucial amplifier of innate rather than acquired immunity","volume":"107","author":"Oboki","year":"2010","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2025010608402852800_r21","doi-asserted-by":"crossref","first-page":"470","DOI":"10.1016\/j.it.2011.05.001","article-title":"Monocyte trafficking in acute and chronic inflammation","volume":"32","author":"Ingersoll","year":"2011","journal-title":"Trends Immunol."},{"key":"2025010608402852800_r22","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1038\/nprot.2014.005","article-title":"Intravascular staining for discrimination of vascular and tissue leukocytes","volume":"9","author":"Anderson","year":"2014","journal-title":"Nat. Protoc."},{"key":"2025010608402852800_r23","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1016\/j.immuni.2012.10.016","article-title":"Conventional and monocyte-derived CD11b(+) dendritic cells initiate and maintain T helper 2 cell-mediated immunity to house dust mite allergen","volume":"38","author":"Plantinga","year":"2013","journal-title":"Immunity"},{"key":"2025010608402852800_r24","doi-asserted-by":"crossref","first-page":"5743","DOI":"10.4049\/jimmunol.0903818","article-title":"IL-33 mediates inflammatory responses in human lung tissue cells","volume":"185","author":"Yagami","year":"2010","journal-title":"J. Immunol."},{"key":"2025010608402852800_r25","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.ejphar.2015.03.015","article-title":"Production of interleukin (IL)-33 in the lungs during multiple antigen challenge-induced airway inflammation in mice, and its modulation by a glucocorticoid","volume":"757","author":"Nabe","year":"2015","journal-title":"Eur. J. Pharmacol."},{"key":"2025010608402852800_r26","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1038\/aps.2011.200","article-title":"Role of G protein-coupled receptors in inflammation","volume":"33","author":"Sun","year":"2012","journal-title":"Acta Pharmacol. Sin."},{"key":"2025010608402852800_r27","doi-asserted-by":"crossref","first-page":"11","DOI":"10.4049\/jimmunol.171.1.11","article-title":"Chemokine receptors in asthma: searching for the correct immune targets","volume":"171","author":"Lukacs","year":"2003","journal-title":"J. Immunol."},{"key":"2025010608402852800_r28","doi-asserted-by":"crossref","first-page":"1073","DOI":"10.1016\/S0140-6736(08)61449-X","article-title":"New targets for drug development in asthma","volume":"372","author":"Adcock","year":"2008","journal-title":"Lancet"},{"key":"2025010608402852800_r29","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1016\/j.trsl.2015.08.004","article-title":"Targeted anti-inflammatory therapeutics in asthma and chronic obstructive lung disease","volume":"167","author":"Durham","year":"2016","journal-title":"Transl. Res."},{"key":"2025010608402852800_r30","doi-asserted-by":"crossref","first-page":"726","DOI":"10.1038\/nm.2768","article-title":"Viral infections and atopy in asthma pathogenesis: new rationales for asthma prevention and treatment","volume":"18","author":"Holt","year":"2012","journal-title":"Nat. Med."}],"container-title":["ImmunoHorizons"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/immunohorizons\/article-pdf\/1\/6\/101\/60185344\/ih1700024.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/immunohorizons\/article-pdf\/1\/6\/101\/60185344\/ih1700024.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,6]],"date-time":"2025-01-06T08:41:44Z","timestamp":1736152904000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/immunohorizons\/article\/1\/6\/101\/7823436"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,8,1]]},"references-count":30,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2017,8,1]]}},"URL":"https:\/\/doi.org\/10.4049\/immunohorizons.1700024","relation":{},"ISSN":["2573-7732"],"issn-type":[{"value":"2573-7732","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2017,8]]},"published":{"date-parts":[[2017,8,1]]}}}