{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,10]],"date-time":"2026-01-10T01:56:07Z","timestamp":1768010167025,"version":"3.49.0"},"reference-count":24,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2025,1,6]],"date-time":"2025-01-06T00:00:00Z","timestamp":1736121600000},"content-version":"vor","delay-in-days":2472,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2018,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>PYNOD (also called NLRP10) is a member of the nucleotide-binding domain and leucine-rich repeat containing family. Many members of this family play important roles in the activation and\/or regulation of immune and inflammatory responses. We previously showed that PYNOD inhibits the IL-1\u03b2 secretion in response to microbial infection in PYNOD-transgenic mice. In this study, we generated PYNOD-knockout (KO) mice and further investigated PYNOD\u2019s role in the innate and adaptive immune responses. Similar to wild-type macrophages, PYNOD-KO macrophages produced IL-1\u03b2 and induced pyroptosis, a caspase-1\u2013dependent programmed cell death, in response to various inflammasome activators and microbial infection. In addition, the PYNOD deficiency did not significantly affect the proliferation or cytokine production of T cells, the delayed-type hypersensitivity responses, the anti-tumor immunity, the Ag-specific Ab production, the cytotoxicity of NK cells, or the maturation, Ag-presenting capacity, or elicited migration of dendritic cells. Furthermore, the steady-state skin self-antigen transport to regional lymph nodes was not impaired in PYNOD-KO mice, suggesting that PYNOD is dispensable for steady-state dendritic cell migration. These results suggested that PYNOD is dispensable for the regulation of innate and adaptive immune responses in mice, unless PYNOD\u2019s expression is highly induced under certain conditions.<\/jats:p>","DOI":"10.4049\/immunohorizons.1700074","type":"journal-article","created":{"date-parts":[[2018,4,30]],"date-time":"2018-04-30T13:05:14Z","timestamp":1525093514000},"page":"129-141","source":"Crossref","is-referenced-by-count":4,"title":["Characterization of Innate and Adaptive Immune Responses in PYNOD-Deficient Mice"],"prefix":"10.1093","volume":"2","author":[{"given":"Shinsuke","family":"Nakajima","sequence":"first","affiliation":[{"name":"Division of Immunology and Molecular Biology, Cancer Research Institute, Kanazawa University , Kanazawa, Ishikawa 920-1192 ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryu","family":"Imamura","sequence":"additional","affiliation":[{"name":"Division of Immunology and Molecular Biology, Cancer Research Institute, Kanazawa University , Kanazawa, Ishikawa 920-1192 ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miya","family":"Yoshino","sequence":"additional","affiliation":[{"name":"Division of Immunology, Department of Molecular and Cellular Biology, School of Life Science, Faculty of Medicine, Tottori University , Yonago, Tottori 683-8503 ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mayumi","family":"Sakurai","sequence":"additional","affiliation":[{"name":"Division of Immunology and Molecular Biology, Cancer Research Institute, Kanazawa University , Kanazawa, Ishikawa 920-1192 ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kohsuke","family":"Tsuchiya","sequence":"additional","affiliation":[{"name":"Division of Immunology and Molecular Biology, Cancer Research Institute, Kanazawa University , Kanazawa, Ishikawa 920-1192 ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazushi","family":"Sugihara","sequence":"additional","affiliation":[{"name":"Division of Transgenic Animal Science, Advanced Science Research Center, Kanazawa University , Kanazawa, Ishikawa 920-8640 ,","place":["Japan"]},{"name":"Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University , Kyoto 606-8501 ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masahide","family":"Asano","sequence":"additional","affiliation":[{"name":"Division of Transgenic Animal Science, Advanced Science Research Center, Kanazawa University , Kanazawa, Ishikawa 920-8640 ,","place":["Japan"]},{"name":"Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University , Kyoto 606-8501 ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9344-5524","authenticated-orcid":false,"given":"Takashi","family":"Suda","sequence":"additional","affiliation":[{"name":"Division of Immunology and Molecular Biology, Cancer Research Institute, Kanazawa University , Kanazawa, Ishikawa 920-1192 ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2018,5,1]]},"reference":[{"key":"2025010608283835300_r1","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1093\/intimm\/dxh081","article-title":"PYNOD, a novel Apaf-1\/CED4-like protein is an inhibitor of ASC and caspase-1","volume":"16","author":"Wang","year":"2004","journal-title":"Int. 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