{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T05:27:53Z","timestamp":1787030873807,"version":"build-2736575974"},"reference-count":27,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2025,1,6]],"date-time":"2025-01-06T00:00:00Z","timestamp":1736121600000},"content-version":"vor","delay-in-days":1801,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Erythema nodosum leprosum (ENL) is an inflammatory complication in leprosy. Yet, the involvement of ENL neutrophils in the inflammatory response against Mycobacterium leprae remains poorly explored. Our primary aim was to investigate the utility of the surface expression of neutrophil IL-10R1 as an ENL biomarker and, secondarily, to evaluate whether leprosy or healthy M. leprae\u2013stimulated neutrophils produce cytokines and are able to respond to IL-10. We, in this study, describe a subpopulation of circulating neutrophils of ENL patients that exclusively expressed IL-10R1, providing evidence that IL-10R1+ neutrophils are present in ENL lesions. It was also found that ENL neutrophils, but not those of nonreactional leprosy controls, were able to secret detectable levels of TNF ex vivo and the addition of IL-10 blocked TNF release. It was likewise observed that M. leprae\u2013stimulated, healthy neutrophils expressed IL-10R1 in vitro, and ENL-linked cytokines were released by M. leprae\u2013cultured neutrophils in vitro. Moreover, consistent with the presence of a fully functional IL-10R, the addition of IL-10 prevented the release of M. leprae\u2013induced cytokines. Most importantly, dead M. leprae revealed its superior capacity to induce CCL4 and IL-8 in primary neutrophils over live Mycobacterium, suggesting that M. leprae may hamper the inflammatory machinery as an immune escape mechanism.<\/jats:p>","DOI":"10.4049\/immunohorizons.1900088","type":"journal-article","created":{"date-parts":[[2020,2,7]],"date-time":"2020-02-07T16:24:23Z","timestamp":1581092663000},"page":"47-56","source":"Crossref","is-referenced-by-count":13,"title":["Erythema Nodosum Leprosum Neutrophil Subset Expressing IL-10R1 Transmigrates into Skin Lesions and Responds to IL-10"],"prefix":"10.1093","volume":"4","author":[{"given":"Fabiana Santos","family":"Pacheco","sequence":"first","affiliation":[{"name":"Leprosy Laboratory, Oswaldo Cruz Institute, FIOCRUZ , Rio de Janeiro, Rio de Janeiro 21040-900 ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rhana Berto da Silva","family":"Prata","sequence":"additional","affiliation":[{"name":"Leprosy Laboratory, Oswaldo Cruz Institute, FIOCRUZ , Rio de Janeiro, Rio de Janeiro 21040-900 ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheila Santos","family":"Brand\u00e3o","sequence":"additional","affiliation":[{"name":"Leprosy Laboratory, Oswaldo Cruz Institute, FIOCRUZ , Rio de Janeiro, Rio de Janeiro 21040-900 ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3596-5035","authenticated-orcid":false,"given":"Helen","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Leprosy Laboratory, Oswaldo Cruz Institute, FIOCRUZ , Rio de Janeiro, Rio de Janeiro 21040-900 ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tha\u00eds Fernanda","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Cellular Microbiology Laboratory, Oswaldo Cruz Institute, FIOCRUZ , Rio de Janeiro, Rio de Janeiro 21040-900 ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2245-0841","authenticated-orcid":false,"given":"J\u00e9ssica","family":"Brand\u00e3o dos Santos","sequence":"additional","affiliation":[{"name":"Leprosy Laboratory, Oswaldo Cruz Institute, FIOCRUZ , Rio de Janeiro, Rio de Janeiro 21040-900 ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camila Oliveira","family":"da Silva","sequence":"additional","affiliation":[{"name":"Cellular Microbiology Laboratory, Oswaldo Cruz Institute, FIOCRUZ , Rio de Janeiro, Rio de Janeiro 21040-900 ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabella Forasteiro","family":"Tavares","sequence":"additional","affiliation":[{"name":"Leprosy Laboratory, Oswaldo Cruz Institute, FIOCRUZ , Rio de Janeiro, Rio de Janeiro 21040-900 ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mayara Abud","family":"Mendes","sequence":"additional","affiliation":[{"name":"Leprosy Laboratory, Oswaldo Cruz Institute, FIOCRUZ , Rio de Janeiro, Rio de Janeiro 21040-900 ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Carolina Duarte Pereira","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Leprosy Laboratory, Oswaldo Cruz Institute, FIOCRUZ , Rio de Janeiro, Rio de Janeiro 21040-900 ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alice de Miranda","family":"Machado","sequence":"additional","affiliation":[{"name":"Leprosy Laboratory, Oswaldo Cruz Institute, FIOCRUZ , Rio de Janeiro, Rio de Janeiro 21040-900 ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Augusto da Costa","family":"Nery","sequence":"additional","affiliation":[{"name":"Leprosy Laboratory, Oswaldo Cruz Institute, FIOCRUZ , Rio de Janeiro, Rio de Janeiro 21040-900 ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2717-4786","authenticated-orcid":false,"given":"Tha\u00eds Porto","family":"Amadeu","sequence":"additional","affiliation":[{"name":"Laboratory of Immunopathology, Faculty of Medical Sciences, State University of Rio de Janeiro , Rio de Janeiro, Rio de Janeiro 20550-900 ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2653-0037","authenticated-orcid":false,"given":"Milton Oz\u00f3rio","family":"Moraes","sequence":"additional","affiliation":[{"name":"Leprosy Laboratory, Oswaldo Cruz Institute, FIOCRUZ , Rio de Janeiro, Rio de Janeiro 21040-900 ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Euzenir Nunes","family":"Sarno","sequence":"additional","affiliation":[{"name":"Leprosy Laboratory, Oswaldo Cruz Institute, FIOCRUZ , Rio de Janeiro, Rio de Janeiro 21040-900 ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0865-133X","authenticated-orcid":false,"given":"Veronica","family":"Schmitz","sequence":"additional","affiliation":[{"name":"Leprosy Laboratory, Oswaldo Cruz Institute, FIOCRUZ , Rio de Janeiro, Rio de Janeiro 21040-900 ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,2,8]]},"reference":[{"key":"2025010608421110300_r1","first-page":"255","article-title":"Classification of leprosy according to immunity. 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