{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T11:43:47Z","timestamp":1792669427138,"version":"4.5.0"},"reference-count":55,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2025,1,6]],"date-time":"2025-01-06T00:00:00Z","timestamp":1736121600000},"content-version":"vor","delay-in-days":1558,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Dengue virus (DENV) is a significant cause of morbidity in many regions of the world, with children at the greatest risk of developing severe dengue. NK cells, characterized by their ability to rapidly recognize and kill virally infected cells, are activated during acute DENV infection. However, their role in viral clearance versus pathogenesis has not been fully elucidated. Our goal was to profile the NK cell receptor\u2013ligand repertoire to provide further insight into the function of NK cells during pediatric and adult DENV infection. We used mass cytometry to phenotype isolate NK cells and PBMCs from a cohort of DENV-infected children and adults. Using unsupervised clustering, we found that pediatric DENV infection leads to a decrease in total NK cell frequency with a reduction in the percentage of CD56dimCD38bright NK cells and an increase in the percentage of CD56dimperforinbright NK cells. No such changes were observed in adults. Next, we identified markers predictive of DENV infection using a differential state test. In adults, NK cell expression of activation markers, including CD69, perforin, and Fas-L, and myeloid cell expression of activating NK cell ligands, namely Fas, were predictive of infection. In contrast, increased NK cell expression of the maturation marker CD57 and myeloid cell expression of inhibitory ligands, such as HLA class I molecules, were predictive of pediatric DENV infection. These findings suggest that acute pediatric DENV infection may result in diminished NK cell activation, which could contribute to enhanced pathogenesis and disease severity.<\/jats:p>","DOI":"10.4049\/immunohorizons.2000074","type":"journal-article","created":{"date-parts":[[2020,10,16]],"date-time":"2020-10-16T17:45:17Z","timestamp":1602870317000},"page":"634-647","source":"Crossref","is-referenced-by-count":10,"title":["Mass Cytometry Analysis of the NK Cell Receptor\u2013Ligand Repertoire Reveals Unique Differences between Dengue-Infected Children and Adults"],"prefix":"10.1093","volume":"4","author":[{"given":"Julia L","family":"McKechnie","sequence":"first","affiliation":[{"name":"Program in Immunology, Stanford University School of Medicine , Stanford, CA 94305"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Davis","family":"Beltr\u00e1n","sequence":"additional","affiliation":[{"name":"Department of Research in Virology and Biotechnology, Gorgas Memorial Institute for Health Studies , Panama City ,","place":["Panama"]},{"name":"Institute for Scientific Research and Technology Services , Panama City ,","place":["Panama"]},{"name":"Department of Biotechnology, Acharya Nagarjuna University , Guntur 522002 ,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne-Maud M","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Department of Statistics, Stanford University , Stanford, CA 94305"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosemary","family":"Vergara","sequence":"additional","affiliation":[{"name":"Department of Medicine, Stanford University School of Medicine , Stanford, CA 94305"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisseth","family":"Saenz","sequence":"additional","affiliation":[{"name":"Department of Research in Virology and Biotechnology, Gorgas Memorial Institute for Health Studies , Panama City ,","place":["Panama"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ofelina","family":"Vergara","sequence":"additional","affiliation":[{"name":"Hospital del Ni\u00f1o Doctor Jos\u00e9 Ren\u00e1n Esquivel , Panama City ,","place":["Panama"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2042-0817","authenticated-orcid":false,"given":"Dora","family":"Estripeaut","sequence":"additional","affiliation":[{"name":"Hospital del Ni\u00f1o Doctor Jos\u00e9 Ren\u00e1n Esquivel , Panama City ,","place":["Panama"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5880-8908","authenticated-orcid":false,"given":"Ana B","family":"Ara\u00faz","sequence":"additional","affiliation":[{"name":"Hospital Santo Tomas , Panama City ,","place":["Panama"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura J","family":"Simpson","sequence":"additional","affiliation":[{"name":"Department of Medicine, Stanford University School of Medicine , Stanford, CA 94305"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2208-8168","authenticated-orcid":false,"given":"Susan","family":"Holmes","sequence":"additional","affiliation":[{"name":"Department of Statistics, Stanford University , Stanford, CA 94305"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1106-8479","authenticated-orcid":false,"given":"Sandra","family":"L\u00f3pez-Verg\u00e8s","sequence":"additional","affiliation":[{"name":"Department of Research in Virology and Biotechnology, Gorgas Memorial Institute for Health Studies , Panama City ,","place":["Panama"]},{"name":"Institute for Scientific Research and Technology Services , Panama City ,","place":["Panama"]},{"name":"Universidad de Panama , Panama City ,","place":["Panama"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine A","family":"Blish","sequence":"additional","affiliation":[{"name":"Program in Immunology, Stanford University School of Medicine , Stanford, CA 94305"},{"name":"Department of Medicine, Stanford University School of Medicine , Stanford, CA 94305"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,10,19]]},"reference":[{"key":"2025010608424249300_r1","volume-title":"EPIDEMIOLOGY, BURDEN OF DISEASE AND TRANSMISSION","author":"World Health Organization","year":"2009"},{"key":"2025010608424249300_r2","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.4269\/ajtmh.2005.73.1063","article-title":"Differences in dengue severity in infants, children, and adults in a 3-year hospital-based study in Nicaragua","volume":"73","author":"Hammond","year":"2005","journal-title":"Am. 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