{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T05:16:46Z","timestamp":1781155006805,"version":"3.54.1"},"reference-count":44,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2025,1,7]],"date-time":"2025-01-07T00:00:00Z","timestamp":1736208000000},"content-version":"vor","delay-in-days":1102,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Our previous studies have shown that ethanol intoxication combined with burn injury increases intestinal bacterial growth, disrupts the intestinal barrier, and enhances bacterial translocation. Additionally, studies show that Th17 effector cytokines IL-17 and IL-22, which are dependent on IL-23, play important roles in maintaining intestine mucosal barrier integrity. Recent findings suggest neutrophils are a significant source of IL-17 and IL-22. We determined the effect of ethanol and burn injury on neutrophil IL-17 and IL-22 production, as well as their ability to phagocytose and in bacterial clearance, and whether these effects are modulated by IL-23. Mice were given ethanol 4 h prior to receiving \u223c12.5% total body surface area burn and were euthanized day 1 after injury. We observed that intoxication combined with burn injury significantly decreases blood neutrophil phagocytosis and bacteria killing, as well as their ability to produce IL-17 and IL-22, compared with sham vehicle mice. The treatment of neutrophils with rIL-23 significantly increases IL-22 and IL-17 release and promotes expression of IL-23R, retinoic acid\u2013related orphan receptor \u03b3t, Lipocalin2, and Nod-like receptor 2 following ethanol and burn injury. Furthermore, IL-22\u2013 and IL-17\u2013producing neutrophils have enhanced neutrophil extracellular trap formation and bacterial killing ability, which are dependent on IL-23. Finally, although we observed that peritoneal neutrophils harvested after casein treatment are functionally different from blood neutrophils, both blood and peritoneal neutrophils exhibited the same response to rIL-23 treatment. Together these findings suggest that IL-23 promotes neutrophil IL-22 and IL-17 production and their ability to kill bacteria following ethanol and burn injury.<\/jats:p>","DOI":"10.4049\/immunohorizons.2100109","type":"journal-article","created":{"date-parts":[[2022,1,20]],"date-time":"2022-01-20T15:20:16Z","timestamp":1642692016000},"page":"64-75","source":"Crossref","is-referenced-by-count":13,"title":["IL-23 Promotes Neutrophil Extracellular Trap Formation and Bacterial Clearance in a Mouse Model of Alcohol and Burn Injury"],"prefix":"10.1093","volume":"6","author":[{"given":"Xiaoling","family":"Li","sequence":"first","affiliation":[{"name":"Alcohol Research Program, Stritch School of Medicine, Loyola University Chicago Health Sciences Division , Maywood, IL"},{"name":"Burn and Shock Trauma Research Institute, Stritch School of Medicine, Loyola University Chicago Health Sciences Division , Maywood, IL"},{"name":"Department of Surgery, Stritch School of Medicine, Loyola University Chicago Health Sciences Division , Maywood, IL"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marisa E","family":"Luck","sequence":"additional","affiliation":[{"name":"Alcohol Research Program, Stritch School of Medicine, Loyola University Chicago Health Sciences Division , Maywood, IL"},{"name":"Burn and Shock Trauma Research Institute, Stritch School of Medicine, Loyola University Chicago Health Sciences Division , Maywood, IL"},{"name":"Integrative Cell Biology Program, Stritch School of Medicine, Loyola University Chicago Health Sciences Division , Maywood, IL"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6345-3758","authenticated-orcid":false,"given":"Caroline J","family":"Herrnreiter","sequence":"additional","affiliation":[{"name":"Alcohol Research Program, Stritch School of Medicine, Loyola University Chicago Health Sciences Division , Maywood, IL"},{"name":"Burn and Shock Trauma Research Institute, Stritch School of Medicine, Loyola University Chicago Health Sciences Division , Maywood, IL"},{"name":"Biochemistry and Molecular Biology Program, Stritch School of Medicine, Loyola University Chicago Health Sciences Division , Maywood, IL"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6590-3759","authenticated-orcid":false,"given":"Abigail R","family":"Cannon","sequence":"additional","affiliation":[{"name":"Alcohol Research Program, Stritch School of Medicine, Loyola University Chicago Health Sciences Division , Maywood, IL"},{"name":"Burn and Shock Trauma Research Institute, Stritch School of Medicine, Loyola University Chicago Health Sciences Division , Maywood, IL"},{"name":"Department of Surgery, Stritch School of Medicine, Loyola University Chicago Health Sciences Division , Maywood, IL"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mashkoor A","family":"Choudhry","sequence":"additional","affiliation":[{"name":"Alcohol Research Program, Stritch School of Medicine, Loyola University Chicago Health Sciences Division , Maywood, IL"},{"name":"Burn and Shock Trauma Research Institute, Stritch School of Medicine, Loyola University Chicago Health Sciences Division , Maywood, IL"},{"name":"Department of Surgery, Stritch School of Medicine, Loyola University Chicago Health Sciences Division , Maywood, IL"},{"name":"Integrative Cell Biology Program, Stritch School of Medicine, Loyola University Chicago Health Sciences Division , Maywood, IL"},{"name":"Biochemistry and Molecular Biology Program, Stritch School of Medicine, Loyola University Chicago Health Sciences Division , Maywood, IL"},{"name":"Department of Microbiology and Immunology, Stritch School of Medicine, Loyola University Chicago Health Sciences Division , Maywood, IL"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,1,1]]},"reference":[{"key":"2025010703505274100_r1","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1097\/BCR.0b013e3182644c58","article-title":"Implications of alcohol intoxication at the time of burn and smoke inhalation injury: an epidemiologic and clinical analysis","volume":"34","author":"Davis","year":"2013","journal-title":"J. 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