{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T05:24:30Z","timestamp":1791523470971,"version":"4.3.4"},"reference-count":86,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2025,1,7]],"date-time":"2025-01-07T00:00:00Z","timestamp":1736208000000},"content-version":"vor","delay-in-days":768,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Hematopoiesis integrates cytokine signaling, metabolism, and epigenetic modifications to regulate blood cell generation. These processes are linked, as metabolites provide essential substrates for epigenetic marks. In this study, we demonstrate that ATP citrate lyase (Acly), which metabolizes citrate to generate cytosolic acetyl-CoA and is of clinical interest, can regulate chromatin accessibility to limit myeloid differentiation. Acly was tested for a role in murine hematopoiesis by small-molecule inhibition or genetic deletion in lineage-depleted, c-Kit\u2013enriched hematopoietic stem and progenitor cells from Mus musculus. Treatments increased the abundance of cell populations that expressed the myeloid integrin CD11b and other markers of myeloid differentiation. When single-cell RNA sequencing was performed, we found that Acly inhibitor\u2013treated hematopoietic stem and progenitor cells exhibited greater gene expression signatures for macrophages and enrichment of these populations. Similarly, the single-cell assay for transposase-accessible chromatin sequencing showed increased chromatin accessibility at genes associated with myeloid differentiation, including CD11b, CD11c, and IRF8. Mechanistically, Acly deficiency altered chromatin accessibility and expression of multiple C\/EBP family transcription factors known to regulate myeloid differentiation and cell metabolism, with increased Cebpe and decreased Cebpa and Cebpb. This effect of Acly deficiency was accompanied by altered mitochondrial metabolism with decreased mitochondrial polarization but increased mitochondrial content and production of reactive oxygen species. The bias to myeloid differentiation appeared due to insufficient generation of acetyl-CoA, as exogenous acetate to support alternate compensatory pathways to produce acetyl-CoA reversed this phenotype. Acly inhibition thus can promote myelopoiesis through deprivation of acetyl-CoA and altered histone acetylome to regulate C\/EBP transcription factor family activity for myeloid differentiation.<\/jats:p>","DOI":"10.4049\/immunohorizons.2200086","type":"journal-article","created":{"date-parts":[[2023,1,3]],"date-time":"2023-01-03T05:05:15Z","timestamp":1672722315000},"page":"837-850","source":"Crossref","is-referenced-by-count":9,"title":["Acly Deficiency Enhances Myelopoiesis through Acetyl Coenzyme A and Metabolic\u2013Epigenetic Cross-Talk"],"prefix":"10.1093","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0905-5211","authenticated-orcid":false,"given":"Dalton L","family":"Greenwood","sequence":"first","affiliation":[{"name":"Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center , Nashville, TN"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haley 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(Amst.)"},{"key":"2025010703535828300_r46","doi-asserted-by":"crossref","first-page":"159018","DOI":"10.1016\/j.bbalip.2021.159018","article-title":"The effects of neuronostatin on proliferation and differentiation of rat primary preadipocytes and 3T3-L1 cells","volume":"1866","author":"Jasaszwili","year":"2021","journal-title":"Biochim. Biophys. Acta Mol. Cell Biol. Lipids"},{"key":"2025010703535828300_r47","doi-asserted-by":"crossref","first-page":"116897","DOI":"10.1016\/j.lfs.2019.116897","article-title":"Regulation of adipocyte differentiation and metabolism by lansoprazole","volume":"239","author":"Benchamana","year":"2019","journal-title":"Life Sci."},{"key":"2025010703535828300_r48","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1182\/blood.V85.2.319.319","article-title":"The CD11b promoter directs high-level expression of reporter genes in macrophages in transgenic mice. 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Hypolipidaemic effects of SB-204990, a lactone prodrug of the potent ATP citrate-lyase inhibitor SB-201076","volume":"334","author":"Pearce","year":"1998","journal-title":"Biochem. J."},{"key":"2025010703535828300_r52","doi-asserted-by":"crossref","first-page":"642229","DOI":"10.3389\/fonc.2021.642229","article-title":"Targeting ACLY attenuates tumor growth and acquired cisplatin resistance in ovarian cancer by inhibiting the PI3K-AKT pathway and activating the AMPK-ROS pathway. [Published erratum appears in 2021 Front. Oncol. 11: 742374.]","volume":"11","author":"Wei","year":"2021","journal-title":"Front. Oncol."},{"key":"2025010703535828300_r53","doi-asserted-by":"crossref","first-page":"8262","DOI":"10.1038\/s41598-019-44720-3","article-title":"Hematopoietic stem and progenitor cell proliferation and differentiation requires the trithorax protein Ash2l","volume":"9","author":"L\u00fcscher-Firzlaff","year":"2019","journal-title":"Sci. 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Med."},{"key":"2025010703535828300_r80","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1038\/icb.1966.28","article-title":"The growth of mouse bone marrow cells in vitro","volume":"44","author":"Bradley","year":"1966","journal-title":"Aust. J. Exp. Biol. Med. Sci."},{"key":"2025010703535828300_r81","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1182\/blood.V60.2.352.352","article-title":"Clonal assay of mouse mast cell colonies in methylcellulose culture","volume":"60","author":"Nakahata","year":"1982","journal-title":"Blood"},{"key":"2025010703535828300_r82","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1182\/blood.V48.3.407.407","article-title":"Human marrow erythropoiesis in culture. I. 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