{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T06:41:59Z","timestamp":1793256119839,"version":"4.5.0"},"reference-count":11,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2025,1,7]],"date-time":"2025-01-07T00:00:00Z","timestamp":1736208000000},"content-version":"vor","delay-in-days":433,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>CD45.1\/CD45.2 congenic markers have been used to track hematopoietic lineage differentiation following hematopoietic stem and progenitor cell (HSPC) transplantation. However, several studies suggest that a bias exists in CD45.1 versus CD45.2 hematopoietic cell reconstitution from HSPCs. Meanwhile, no definitive comparison has been reported for mature immune cells as to whether the CD45.1\/CD45.2 disparity can skew the immune cell response. In this study, using lymphocytopenia Rag1\u2212\/\u2212 CD45.2 mice as hosts, we assessed the reconstitution potential of CD45.1 versus CD45.2 lymphocytes following adoptive transfer of mature T and B cells. We have found a selective bias for CD8+ T cells in that CD45.1 cells showed significantly higher reconstitution compared with CD45.2 cells, whereas CD4+ T cells and CD19+ B cells showed equivalent reconstitution. These results suggest that CD45.1\/CD45.2 markers may induce an alloreactive response or a survival bias specific to CD8+ T cells, and they therefore call for caution for using them as congenic markers in immunologic models.<\/jats:p>","DOI":"10.4049\/immunohorizons.2300014","type":"journal-article","created":{"date-parts":[[2023,11,8]],"date-time":"2023-11-08T14:57:22Z","timestamp":1699455442000},"page":"755-759","source":"Crossref","is-referenced-by-count":1,"title":["CD45.1\/CD45.2 Congenic Markers Induce a Selective Bias for CD8+ T Cells during Adoptive Lymphocyte Reconstitution in Lymphocytopenia Mice"],"prefix":"10.1093","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-1102-7929","authenticated-orcid":false,"given":"Rakhee Rathnam","family":"Kalari Kandy","sequence":"first","affiliation":[{"name":"Marlene and Stewart Greenebaum Comprehensive Cancer Center, University of Maryland Baltimore School of Medicine , Baltimore, MD"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0501-771X","authenticated-orcid":false,"given":"Xiaoxuan","family":"Fan","sequence":"additional","affiliation":[{"name":"Marlene and Stewart Greenebaum Comprehensive Cancer Center, University of Maryland Baltimore School of Medicine , Baltimore, MD"},{"name":"Department of Microbiology and Immunology, University of Maryland Baltimore School of Medicine , Baltimore, MD"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuefang","family":"Cao","sequence":"additional","affiliation":[{"name":"Marlene and Stewart Greenebaum Comprehensive Cancer Center, University of Maryland Baltimore School of Medicine , Baltimore, MD"},{"name":"Department of Microbiology and Immunology, University of Maryland Baltimore School of Medicine , Baltimore, MD"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2023,11,8]]},"reference":[{"key":"2025010703571043200_r1","doi-asserted-by":"crossref","first-page":"3495","DOI":"10.1038\/s41598-017-03784-9","article-title":"A sex-specific reconstitution bias in the competitive CD45.1\/CD45.2 congenic bone marrow transplant model","volume":"7","author":"Jafri","year":"2017","journal-title":"Sci. Rep."},{"key":"2025010703571043200_r2","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/j.jim.2013.07.008","article-title":"Differential representation of B cell subsets in mixed bone marrow chimera mice due to expression of allelic variants of CD45 (CD45.1\/CD45.2)","volume":"396","author":"Basu","year":"2013","journal-title":"J. Immunol. Methods"},{"key":"2025010703571043200_r3","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1634\/stemcells.19-1-80","article-title":"Immunogenicity of Ly5 (CD45)-antigens hampers long-term engraftment following minimal conditioning in a murine bone marrow transplantation model","volume":"19","author":"van Os","year":"2001","journal-title":"Stem Cells"},{"key":"2025010703571043200_r4","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1016\/j.bbmt.2005.11.011","article-title":"Induction of mixed chimerism through transplantation of CD45-congenic mobilized peripheral blood stem cells after nonmyeloablative irradiation","volume":"12","author":"Koporc","year":"2006","journal-title":"Biol. Blood Marrow Transplant."},{"key":"2025010703571043200_r5","doi-asserted-by":"crossref","first-page":"1039","DOI":"10.1634\/stemcells.22-6-1039","article-title":"CD45 congenic bone marrow transplantation: evidence for T cell-mediated immunity","volume":"22","author":"Xu","year":"2004","journal-title":"Stem Cells"},{"key":"2025010703571043200_r6","doi-asserted-by":"crossref","first-page":"3700","DOI":"10.4049\/jimmunol.1700380","article-title":"T cell-derived CD70 delivers an immune checkpoint function in inflammatory T cell responses","volume":"199","author":"O\u2019Neill","year":"2017","journal-title":"J. Immunol."},{"key":"2025010703571043200_r7","doi-asserted-by":"crossref","first-page":"1982","DOI":"10.4049\/jimmunol.1701676","article-title":"Cutting edge: check your mice\u2014a point mutation in the Ncr1 locus identified in CD45.1 congenic mice with consequences in mouse susceptibility to infection","volume":"200","author":"Jang","year":"2018","journal-title":"J. Immunol."},{"key":"2025010703571043200_r8","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1038\/icb.1997.68","article-title":"The role of CD45 and CD45-associated molecules in T cell activation","volume":"75","author":"Altin","year":"1997","journal-title":"Immunol. Cell Biol."},{"key":"2025010703571043200_r9","doi-asserted-by":"crossref","first-page":"1138","DOI":"10.4049\/jimmunol.149.4.1138","article-title":"Restoration of T cell receptor-mediated signal transduction by transfection of CD45 cDNA into a CD45-deficient variant of the Jurkat T cell line","volume":"149","author":"Koretzky","year":"1992","journal-title":"J. Immunol."},{"key":"2025010703571043200_r10","doi-asserted-by":"crossref","first-page":"408","DOI":"10.1182\/blood-2008-03-143370","article-title":"Congenic interval of CD45\/Ly-5 congenic mice contains multiple genes that may influence hematopoietic stem cell engraftment","volume":"115","author":"Waterstrat","year":"2010","journal-title":"Blood"},{"key":"2025010703571043200_r11","doi-asserted-by":"crossref","first-page":"985","DOI":"10.1016\/j.stemcr.2016.04.010","article-title":"Single targeted exon mutation creates a true congenic mouse for competitive hematopoietic stem cell transplantation: the C57BL\/6-CD45.1STEM mouse","volume":"6","author":"Mercier","year":"2016","journal-title":"Stem Cell Reports"}],"container-title":["ImmunoHorizons"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/immunohorizons\/article-pdf\/7\/11\/755\/60188142\/ih2300014.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/immunohorizons\/article-pdf\/7\/11\/755\/60188142\/ih2300014.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,7]],"date-time":"2025-01-07T04:07:03Z","timestamp":1736222823000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/immunohorizons\/article\/7\/11\/755\/7816907"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,11,1]]},"references-count":11,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2023,11,1]]}},"URL":"https:\/\/doi.org\/10.4049\/immunohorizons.2300014","relation":{},"ISSN":["2573-7732"],"issn-type":[{"value":"2573-7732","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2023,11]]},"published":{"date-parts":[[2023,11,1]]}}}