{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T23:54:13Z","timestamp":1769644453142,"version":"3.49.0"},"reference-count":33,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2009,2,1]],"date-time":"2009-02-01T00:00:00Z","timestamp":1233446400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2009,2,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Salmonella organisms are Gram negative and facultative anaerobic bacteria that cause typhoid fever in humans. In this study, we evaluated LPS-specific adaptive immunity in innate immune-deficient mice after oral administration of attenuated Salmonella enterica serovar Typhimurium (S. Typhimurium) strains. Of interest, identical levels of LPS-specific IgG and IgA Abs were elicited in the systemic (i.e., serum and spleen) and mucosal (i.e., fecal extract and small intestine) compartments of wild-type, TLR4\u2212\/\u2212, and MyD88\u2212\/\u2212 mice following oral vaccination with recombinant attenuated S. Typhimurium (RASV). Depletion of CD4+ T cells during RASV vaccination completely abrogated the generation of LPS-specific Abs in MyD88\u2212\/\u2212 mice. In addition, mRNA expression levels of a B cell-activating factor of the TNF family were significantly increased in the spleens of MyD88\u2212\/\u2212 mice after oral administration, implying that T cell-independent B cell switching might be also enhanced in the MyD88 signal-deficient condition. Of most interest, orally vaccinated MyD88\u2212\/\u2212 mice that possessed high levels of LPS-specific IgG and IgA, which had a neutralizing effect against Salmonella, died earlier than nonvaccinated wild-type mice following lethal oral challenge with virulent Salmonella species. These results suggest that innate immunity mediated by MyD88 signal is dispensable for induction of LPS-specific Ab responses following oral administration of attenuated Salmonella strains but indispensable for efficient protection.<\/jats:p>","DOI":"10.4049\/jimmunol.0801980","type":"journal-article","created":{"date-parts":[[2009,2,9]],"date-time":"2009-02-09T12:02:33Z","timestamp":1234180953000},"page":"2305-2312","source":"Crossref","is-referenced-by-count":25,"title":["Innate Immunity Mediated by MyD88 Signal Is Not Essential for Induction of Lipopolysaccharide-Specific B Cell Responses but Is Indispensable for Protection against\n                    <i>Salmonella enterica<\/i>\n                    serovar\n                    <i>Typhimurium<\/i>\n                    infection"],"prefix":"10.1093","volume":"182","author":[{"given":"Hyun-Jeong","family":"Ko","sequence":"first","affiliation":[{"name":"Mucosal Immunology Section, Laboratory Science Division, International Vaccine Institute , Seoul,","place":["Korea"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jin-Young","family":"Yang","sequence":"additional","affiliation":[{"name":"Mucosal Immunology Section, Laboratory Science Division, International Vaccine Institute , Seoul,","place":["Korea"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Doo-Hee","family":"Shim","sequence":"additional","affiliation":[{"name":"Mucosal Immunology Section, Laboratory Science Division, International Vaccine Institute , Seoul,","place":["Korea"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hyungjun","family":"Yang","sequence":"additional","affiliation":[{"name":"Mucosal Immunology Section, Laboratory Science Division, International Vaccine Institute , Seoul,","place":["Korea"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sung-Moo","family":"Park","sequence":"additional","affiliation":[{"name":"Mucosal Immunology Section, Laboratory Science Division, International Vaccine Institute , Seoul,","place":["Korea"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"III","given":"Roy","family":"Curtiss","sequence":"additional","affiliation":[{"name":"Center for Infectious Diseases and Vaccinology, The Biodesign Institute, Arizona State University , Tempe, AZ 85287"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mi-Na","family":"Kweon","sequence":"additional","affiliation":[{"name":"Mucosal Immunology Section, Laboratory Science Division, International Vaccine Institute , Seoul,","place":["Korea"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2009,2,15]]},"reference":[{"key":"2026012806244539000_R1","first-page":"253","article-title":"Typhoid fever and other salmonellosis: a continuing challenge.","volume-title":"Trends Microbiol.","year":"1995"},{"key":"2026012806244539000_R2","first-page":"393","article-title":"Immunity to systemic Salmonella infections.","volume-title":"Curr. 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