{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T15:04:46Z","timestamp":1770044686988,"version":"3.49.0"},"reference-count":68,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2009,10,1]],"date-time":"2009-10-01T00:00:00Z","timestamp":1254355200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2009,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The PI3K pathway plays a pivotal role in the stimulation of mast cells. PI3K-dependent kinases include the serum- and glucocorticoid-inducible kinase 1 (SGK1). The present study explored the role of SGK1 in mast cell function. Mast cells were isolated from bone marrow (BMMC) of SGK1 knockout mice (sgk1\u2212\/\u2212) and their wild-type littermates (sgk1+\/+). The BMMC number as well as CD117, CD34, and Fc\u03b5RI expression in BMCCs were similar in both genotypes. Upon Ag stimulation of the Fc\u03b5RI receptor, Ca2+ entry but not Ca2+ release from intracellular stores was markedly impaired in sgk1\u2212\/\u2212 BMMCs. The currents through Ca2+-activated K+ channels induced by Ag were significantly higher in sgk1+\/+ BMMCs than in sgk1\u2212\/\u2212 BMMCs. Treatment with the Ca2+ ionophore ionomycin (1 \u03bcM) led to activation of the K+ channels in both genotypes, indicating that the Ca2+-activated K+ channels are similarly expressed and sensitive to activation by Ca2+ in sgk1+\/+ and sgk1\u2212\/\u2212 BMMCs, and that blunted stimulation of Ca2+-activated K+ channels was secondary to decreased Ca2+ entry. Ag-IgE-induced degranulation and early IL-6 secretion were also significantly blunted in sgk1\u2212\/\u2212 BMMCs. The decrease in body temperature following Ag treatment, which reflects an anaphylactic reaction, was substantially reduced in sgk1\u2212\/\u2212 mice, pointing to impaired mast cell function in vivo. Serum histamine levels measured 30 min after induction of an anaphylactic reaction were significantly lower in sgk1\u2212\/\u2212 than in sgk1+\/+mice. The observations reveal a critical role for SGK1 in ion channel regulation and the function of mast cells, and thus disclose a completely novel player in the regulation of allergic reaction.<\/jats:p>","DOI":"10.4049\/jimmunol.0803017","type":"journal-article","created":{"date-parts":[[2009,9,12]],"date-time":"2009-09-12T00:46:53Z","timestamp":1252716413000},"page":"4395-4402","source":"Crossref","is-referenced-by-count":24,"title":["Impaired Mast Cell Activation in Gene-Targeted Mice Lacking the Serum- and Glucocorticoid-Inducible Kinase SGK1"],"prefix":"10.1093","volume":"183","author":[{"given":"Malgorzata","family":"Sobiesiak","sequence":"first","affiliation":[{"name":"Department of Physiology, University of T\u00fcbingen , T\u00fcbingen,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ekaterina","family":"Shumilina","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of T\u00fcbingen , T\u00fcbingen,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rebecca S","family":"Lam","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of T\u00fcbingen , T\u00fcbingen,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Florian","family":"W\u00f6lbing","sequence":"additional","affiliation":[{"name":"Department of Dermatology, University of T\u00fcbingen , T\u00fcbingen,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicole","family":"Matzner","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of T\u00fcbingen , T\u00fcbingen,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susanne","family":"Kaesler","sequence":"additional","affiliation":[{"name":"Department of Dermatology, University of T\u00fcbingen , T\u00fcbingen,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irina M","family":"Zemtsova","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of T\u00fcbingen , T\u00fcbingen,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adrian","family":"Lupescu","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of T\u00fcbingen , T\u00fcbingen,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naima","family":"Zahir","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of T\u00fcbingen , T\u00fcbingen,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dietmar","family":"Kuhl","sequence":"additional","affiliation":[{"name":"Center for Molecular Neurobiology, Institute for Molecular and Cellular Cognition, University Medical Center Hamburg-Eppendorf , Hamburg,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin","family":"Schaller","sequence":"additional","affiliation":[{"name":"Department of Dermatology, University of T\u00fcbingen , T\u00fcbingen,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tilo","family":"Biedermann","sequence":"additional","affiliation":[{"name":"Department of Dermatology, University of T\u00fcbingen , T\u00fcbingen,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Florian","family":"Lang","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of T\u00fcbingen , T\u00fcbingen,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2009,10,1]]},"reference":[{"key":"2025032603061338500_R1","first-page":"773","article-title":"Regulation of mast-cell and basophil function and survival by IgE.","volume-title":"Nat. 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