{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T16:41:19Z","timestamp":1790613679221,"version":"4.1.0"},"reference-count":33,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2009,3,1]],"date-time":"2009-03-01T00:00:00Z","timestamp":1235865600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2009,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The Foxp3-expressing subset of regulatory CD4+ T cells have defined Ag specificity and play essential roles in maintaining peripheral tolerance by suppressing the activation of self-reactive T cells. Similarly, during chronic infection, pathogen-specific Foxp3-expressing CD4+ T cells expand and actively suppress pathogen-specific effector T cells. Herein, we used MHC class II tetramers and Foxp3gfp knockin mice to track the kinetics and magnitude whereby pathogen-specific Foxp3+CD4+ and Foxp3\u2212CD4+ cells are primed and expand after acute infection with recombinant Listeria monocytogenes (Lm) expressing the non-\u201cself\u201d-Ag 2W1S52\u201368. We demonstrate that Lm infection selectively primes proliferation, expansion, and subsequent contraction of Lm-specific Foxp3\u2212 effector CD4+ cells, while the numbers of Lm-specific Foxp3+CD4+ regulatory cells remain essentially unchanged. In sharp contrast, purified 2W1S52\u201368 peptide primes coordinated expansion of both Foxp3+ regulatory and Foxp3\u2212 effector T cells with the same Ag specificity. Taken together, these results indicate selective priming and expansion of Foxp3\u2212 CD4 T cells is a distinguishing feature for acute bacterial infection.<\/jats:p>","DOI":"10.4049\/jimmunol.0803402","type":"journal-article","created":{"date-parts":[[2009,2,20]],"date-time":"2009-02-20T17:41:32Z","timestamp":1235151692000},"page":"3032-3038","source":"Crossref","is-referenced-by-count":64,"title":["Selective Priming and Expansion of Antigen-Specific Foxp3\u2212CD4+ T Cells during\n                    <i>Listeria monocytogenes<\/i>\n                    Infection"],"prefix":"10.1093","volume":"182","author":[{"given":"James M","family":"Ertelt","sequence":"first","affiliation":[{"name":"Pediatrics, Microbiology, Center for Infectious Disease and Microbiology Translational Research, University of Minnesota School of Medicine , Minneapolis, MN 55455"},{"name":"Microbiology, Center for Infectious Disease and Microbiology Translational Research, University of Minnesota School of Medicine , Minneapolis, MN 55455"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jared H","family":"Rowe","sequence":"additional","affiliation":[{"name":"Pediatrics, Microbiology, Center for Infectious Disease and Microbiology Translational Research, University of Minnesota School of Medicine , Minneapolis, MN 55455"},{"name":"Microbiology, Center for Infectious Disease and Microbiology Translational Research, University of Minnesota School of Medicine , Minneapolis, MN 55455"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tanner M","family":"Johanns","sequence":"additional","affiliation":[{"name":"Pediatrics, Microbiology, Center for Infectious Disease and Microbiology Translational Research, University of Minnesota School of Medicine , Minneapolis, MN 55455"},{"name":"Microbiology, Center for Infectious Disease and Microbiology Translational Research, University of Minnesota School of Medicine , Minneapolis, MN 55455"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph C","family":"Lai","sequence":"additional","affiliation":[{"name":"Pediatrics, Microbiology, Center for Infectious Disease and Microbiology Translational Research, University of Minnesota School of Medicine , Minneapolis, MN 55455"},{"name":"Microbiology, Center for Infectious Disease and Microbiology Translational Research, University of Minnesota School of Medicine , Minneapolis, MN 55455"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James B","family":"McLachlan","sequence":"additional","affiliation":[{"name":"Pediatrics, Microbiology, Center for Infectious Disease and Microbiology Translational Research, University of Minnesota School of Medicine , Minneapolis, MN 55455"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sing Sing","family":"Way","sequence":"additional","affiliation":[{"name":"Pediatrics, Microbiology, Center for Infectious Disease and Microbiology Translational Research, University of Minnesota School of Medicine , Minneapolis, MN 55455"},{"name":"Microbiology, Center for Infectious Disease and Microbiology Translational Research, University of Minnesota School of Medicine , Minneapolis, MN 55455"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2009,3,1]]},"reference":[{"key":"2026012807032885100_R1","first-page":"337","article-title":"An essential role for Scurfin in CD4+CD25+ T regulatory cells.","volume-title":"Nat. Immunol.","year":"2003"},{"key":"2026012807032885100_R2","first-page":"68","article-title":"Disruption of a new forkhead\/winged-helix protein, scurfin, results in the fatal lymphoproliferative disorder of the scurfy mouse.","volume-title":"Nat. Genet.","year":"2001"},{"key":"2026012807032885100_R3","first-page":"183","article-title":"Suppressor effector function of CD4+CD25+ immunoregulatory T cells is antigen nonspecific.","volume-title":"J. Immunol.","year":"2000"},{"key":"2026012807032885100_R4","first-page":"287","article-title":"CD4+CD25+ immunoregulatory T cells suppress polyclonal T cell activation in vitro by inhibiting interleukin 2 production.","volume-title":"J. Exp. Med.","year":"1998"},{"key":"2026012807032885100_R5","first-page":"401","article-title":"An intersection between the self-reactive regulatory and nonregulatory T cell receptor repertoires.","volume-title":"Nat. Immunol.","year":"2006"},{"key":"2026012807032885100_R6","first-page":"2149","article-title":"CD4+CD25+ regulatory T cell repertoire formation shaped by differential presentation of peptides from a self-antigen.","volume-title":"J. Immunol.","year":"2008"},{"key":"2026012807032885100_R7","first-page":"6123","article-title":"Human CD4+CD25+ regulatory T cells share equally complex and comparable repertoires with CD4+CD25\u2212 counterparts.","volume-title":"J. Immunol.","year":"2004"},{"key":"2026012807032885100_R8","first-page":"3412","article-title":"Cutting edge: size and diversity of CD4+CD25highFoxp3+ regulatory T cell repertoire in humans: evidence for similarities and partial overlapping with CD4+CD25\u2212 T cells.","volume-title":"J. Immunol.","year":"2007"},{"key":"2026012807032885100_R9","first-page":"5240","article-title":"Antigen-specific T cell repertoire modification of CD4+CD25+ regulatory T cells.","volume-title":"J. Immunol.","year":"2004"},{"key":"2026012807032885100_R10","first-page":"188","article-title":"Generation of anergic and potentially immunoregulatory CD25+CD4 T cells in vivo after induction of peripheral tolerance with intravenous or oral antigen.","volume-title":"J. Immunol.","year":"2001"},{"key":"2026012807032885100_R11","first-page":"3479","article-title":"Induction of antigen-specific regulatory T cells in wild-type mice: visualization and targets of suppression.","volume-title":"Proc. Natl. Acad. Sci. USA","year":"2008"},{"key":"2026012807032885100_R12","first-page":"353","article-title":"Natural regulatory T cells in infectious disease.","volume-title":"Nat. Immunol.","year":"2005"},{"key":"2026012807032885100_R13","first-page":"2159","article-title":"Expansion and function of Foxp3-expressing T regulatory cells during tuberculosis.","volume-title":"J. Exp. Med.","year":"2007"},{"key":"2026012807032885100_R14","first-page":"2661","article-title":"Cutting Edge: regulatory T cells prevent efficient clearance of Mycobacterium tuberculosis.","volume-title":"J. Immunol.","year":"2007"},{"key":"2026012807032885100_R15","first-page":"4123","article-title":"CD4+CD25+ regulatory T cells control the severity of viral immunoinflammatory lesions.","volume-title":"J. Immunol.","year":"2004"},{"key":"2026012807032885100_R16","first-page":"5839","article-title":"Regulatory T cell responses develop in parallel to Th responses and control the magnitude and phenotype of the Th effector population.","volume-title":"J. Immunol.","year":"2006"},{"key":"2026012807032885100_R17","first-page":"777","article-title":"Infected site-restricted Foxp3+ natural regulatory T cells are specific for microbial antigens.","volume-title":"J. Exp. Med.","year":"2006"},{"key":"2026012807032885100_R18","first-page":"3157","article-title":"The pathogenesis of schistosomiasis is controlled by cooperating IL-10-producing innate effector and regulatory T cells.","volume-title":"J. Immunol.","year":"2004"},{"key":"2026012807032885100_R19","first-page":"1224","article-title":"CD25+CD4+ cells contribute to Th2 polarization during helminth infection by suppressing Th1 response development.","volume-title":"J. Immunol.","year":"2004"},{"key":"2026012807032885100_R20","first-page":"502","article-title":"CD4+CD25+ regulatory T cells control Leishmania major persistence and immunity.","volume-title":"Nature","year":"2002"},{"key":"2026012807032885100_R21","first-page":"201","article-title":"Role for CD4+CD25+ regulatory T cells in reactivation of persistent leishmaniasis and control of concomitant immunity.","volume-title":"J. Exp. Med.","year":"2004"},{"key":"2026012807032885100_R22","first-page":"203","article-title":"Naive CD4+ T cell frequency varies for different epitopes and predicts repertoire diversity and response magnitude.","volume-title":"Immunity.","year":"2007"},{"key":"2026012807032885100_R23","first-page":"11890","article-title":"Expression and phosphorylation of the Listeria monocytogenes ActA protein in mammalian cells.","volume-title":"Proc. Natl. Acad. Sci. USA.","year":"1993"},{"key":"2026012807032885100_R24","first-page":"4731","article-title":"Cutting edge: recombinant Listeria monocytogenes expressing a single immune-dominant peptide confers protective immunity to herpes simplex virus-1 infection.","volume-title":"J. Immunol.","year":"2007"},{"key":"2026012807032885100_R25","first-page":"1528","article-title":"Cutting edge: CD4 and CD8 T cells are intrinsically different in their proliferative responses.","volume-title":"J. Immunol.","year":"2002"},{"key":"2026012807032885100_R26","first-page":"329","article-title":"Regulatory T cell lineage specification by the forkhead transcription factor foxp3.","volume-title":"Immunity","year":"2005"},{"key":"2026012807032885100_R27","first-page":"533","article-title":"Cutting edge: protective cell-mediated immunity to Listeria monocytogenes in the absence of myeloid differentiation factor 88.","volume-title":"J. Immunol.","year":"2003"},{"key":"2026012807032885100_R28","first-page":"109","article-title":"Specific immunity to Listeria monocytogenes in the absence of IFN-\u03b3.","volume-title":"Immunity","year":"1995"},{"key":"2026012807032885100_R29","first-page":"3695","article-title":"Induction of protective immunity to Listeria monocytogenes in neonates.","volume-title":"J. Immunol.","year":"2007"},{"key":"2026012807032885100_R30","first-page":"4109","article-title":"Deviation from a strong Th1-dominated to a modest Th17-dominated CD4 T cell response in the absence of IL-12p40 and type I IFNs sustains protective CD8 T cells.","volume-title":"J. Immunol.","year":"2008"},{"key":"2026012807032885100_R31","first-page":"4110","article-title":"Down-regulation of homing receptors after T cell activation.","volume-title":"J. Immunol.","year":"1988"},{"key":"2026012807032885100_R32","first-page":"324","article-title":"Long-term CD4+ memory T cells from the spleen lack MEL-14, the lymph node homing receptor.","volume-title":"J. Immunol.","year":"1992"},{"key":"2026012807032885100_R33","first-page":"1585","article-title":"Regulatory CD4+CD25+ T cells restrict memory CD8+ T cell responses.","volume-title":"J. Exp. Med.","year":"2002"}],"container-title":["The Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/182\/5\/3032\/62683966\/8608.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/182\/5\/3032\/62683966\/8608.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T12:03:44Z","timestamp":1769601824000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jimmunol\/article\/182\/5\/3032\/8007630"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,3,1]]},"references-count":33,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2009,3,1]]},"published-print":{"date-parts":[[2009,3,1]]}},"URL":"https:\/\/doi.org\/10.4049\/jimmunol.0803402","relation":{},"ISSN":["1550-6606","0022-1767"],"issn-type":[{"value":"1550-6606","type":"electronic"},{"value":"0022-1767","type":"print"}],"subject":[],"published-other":{"date-parts":[[2009,3]]},"published":{"date-parts":[[2009,3,1]]}}}