{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T10:07:55Z","timestamp":1790158075175,"version":"4.1.0"},"reference-count":47,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2009,3,1]],"date-time":"2009-03-01T00:00:00Z","timestamp":1235865600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2009,3,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Neutrophil chemotaxis depends on actin dynamics, but the roles for specific cytoskeleton regulators in this response remain unclear. By analysis of mammalian diaphanous-related formin 1 (mDia1)-deficient mice, we have identified an essential role for this actin nucleator in neutrophil chemotaxis. Lack of mDia1 was associated with defects in chemoattractant-induced neutrophil actin polymerization, polarization, and directional migration, and also with impaired activation of RhoA, its downstream target p160-Rho-associated coil-containing protein kinase (ROCK), and the leukemia-associated RhoA guanine nucleotide exchange factor (LARG). Our data also revealed mDia1 to be associated with another cytoskeletal regulator, Wiskott-Aldrich syndrome protein (WASp), at the leading edge of chemotaxing neutrophils and revealed polarized morphology and chemotaxis to be more mildly impaired in WAS\u2212\/\u2212 than in mDia1\u2212\/\u2212 neutrophils, but essentially abrogated by combined mDia1\/WASp deficiency. Thus, mDia1 roles in neutrophil chemotaxis appear to be subserved in concert with WASp and are realized at least in part by activation of the LARG\/RhoA\/ROCK signaling pathway.<\/jats:p>","DOI":"10.4049\/jimmunol.0803838","type":"journal-article","created":{"date-parts":[[2009,3,5]],"date-time":"2009-03-05T20:01:20Z","timestamp":1236283280000},"page":"3837-3845","source":"Crossref","is-referenced-by-count":77,"title":["The mDial Formin Is Required for Neutrophil Polarization, Migration, and Activation of the LARG\/RhoA\/ROCK Signaling Axis during Chemotaxis"],"prefix":"10.1093","volume":"182","author":[{"given":"Yongquan","family":"Shi","sequence":"first","affiliation":[{"name":"Department of Medicine, University of Toronto, Samuel Lunenfeld and Toronto Hospital Research Institutes , Toronto,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinyi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Toronto, Samuel Lunenfeld and Toronto Hospital Research Institutes , Toronto,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Mullin","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Toronto, Samuel Lunenfeld and Toronto Hospital Research Institutes , Toronto,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baoxia","family":"Dong","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Toronto, Samuel Lunenfeld and Toronto Hospital Research Institutes , Toronto,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arthur S","family":"Alberts","sequence":"additional","affiliation":[{"name":"Cell Structure and Signal Integration Laboratory, Van Andel Institute , Grand Rapids, MI 49503"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katherine A","family":"Siminovitch","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Toronto, Samuel Lunenfeld and Toronto Hospital Research Institutes , Toronto,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2009,3,15]]},"reference":[{"key":"2026012807055861200_R1","first-page":"173","article-title":"Neutrophils and immunity: challenges and opportunities.","volume-title":"Nat. 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