{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T14:18:51Z","timestamp":1761488331719,"version":"3.40.3"},"reference-count":40,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2009,11,1]],"date-time":"2009-11-01T00:00:00Z","timestamp":1257033600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2009,11,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Because Mycobacterium bovis bacillus Calmette-Gu\u00e9rin (BCG) unconvincingly activates human naive CD8+ T cells, a rBCG (BCG-70M) that secretes a fusion protein comprising BCG-derived heat shock protein (HSP)70 and Mycobacterium leprae-derived major membrane protein (MMP)-II, one of the immunodominant Ags of M. leprae, was newly constructed to potentiate the ability of activating naive CD8+ T cells through dendritic cells (DC). BCG-70M secreted HSP70-MMP-II fusion protein in vitro, which stimulated DC to produce IL-12p70 through TLR2. BCG-70M-infected DC activated not only memory and naive CD8+ T cells, but also CD4+ T cells of both types to produce IFN-\u03b3. The activation of these naive T cells by BCG-70M was dependent on the MHC and CD86 molecules on BCG-70M-infected DC, and was significantly inhibited by pretreatment of DC with chloroquine. Both brefeldin A and lactacystin significantly inhibited the activation of naive CD8+ T cells by BCG-70M through DC. Thus, the CD8+ T cell activation may be induced by cross-presentation of Ags through a TAP- and proteosome-dependent cytosolic pathway. When naive CD8+ T cells were stimulated by BCG-70M-infected DC in the presence of naive CD4+ T cells, CD62LlowCD8+ T cells and perforin-producing CD8+ T cells were efficiently produced. MMP-II-reactive CD4+ and CD8+ memory T cells were efficiently produced in C57BL\/6 mice by infection with BCG-70M. These results indicate that BCG-70M activated DC, CD4+ T cells, and CD8+ T cells, and the combination of HSP70 and MMP-II may be useful for inducing better T cell activation.<\/jats:p>","DOI":"10.4049\/jimmunol.0803857","type":"journal-article","created":{"date-parts":[[2009,10,22]],"date-time":"2009-10-22T02:25:49Z","timestamp":1256178349000},"page":"6561-6568","source":"Crossref","is-referenced-by-count":21,"title":["Induction of Cross-Priming of Naive CD8+ T Lymphocytes by Recombinant Bacillus Calmette-Gu\u00e9rin That Secretes Heat Shock Protein 70-Major Membrane Protein-II Fusion Protein"],"prefix":"10.1093","volume":"183","author":[{"given":"Tetsu","family":"Mukai","sequence":"first","affiliation":[{"name":"Department of Mycobacteriology, Leprosy Research Center, National Institute of Infectious Diseases , Tokyo,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yumi","family":"Maeda","sequence":"additional","affiliation":[{"name":"Department of Mycobacteriology, Leprosy Research Center, National Institute of Infectious Diseases , Tokyo,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshiki","family":"Tamura","sequence":"additional","affiliation":[{"name":"Department of Mycobacteriology, Leprosy Research Center, National Institute of Infectious Diseases , Tokyo,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masanori","family":"Matsuoka","sequence":"additional","affiliation":[{"name":"Department of Mycobacteriology, Leprosy Research Center, National Institute of Infectious Diseases , Tokyo,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yumiko","family":"Tsukamoto","sequence":"additional","affiliation":[{"name":"Department of Mycobacteriology, Leprosy Research Center, National Institute of Infectious Diseases , Tokyo,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masahiko","family":"Makino","sequence":"additional","affiliation":[{"name":"Department of Mycobacteriology, Leprosy Research Center, National Institute of Infectious Diseases , Tokyo,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2009,11,15]]},"reference":[{"key":"2025032709515187000_R1","first-page":"7771","article-title":"An in vitro model for the lepromatous leprosy granuloma: fate of Mycobacterium leprae from target macrophages after interaction with normal and activated effector macrophages.","volume-title":"J. 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